Gene Transfer to Plants

Yıl 1994, Cilt: 7 Sayı: 1, 101 - 107, 01.12.1994

Kenan Turgut

Öz

In this review, gene transfer methods for plant transformation were discussed. Agrobacterium-mediated gene transfer and direct gene transfer techniques namely microprojectile bombardment, gene transfer to plant protoplasts and DNA enjection were presented comparatively. Furthermore, advantages and disadvantages of each technique were discussed.

Anahtar Kelimeler

Gene Transfer, Agrobacterium, DNA enjection

Bitkilerde Gen Transferi

Öz

Bu derlemede bitkilerin transformasyonunda kullanılan gen transfer yöntemleri tartışılmıştır. Agrobacterium yoluyla gen transferi ve mikroprojekti1 bombardımanı, bitki protoplastlarına gen transferi ve DNA enjeksiyonu gibi doğrudan gen transfer teknikleri karşılaştırmalı olarak sunulmuştur. Bunlara ilaveten her bir tekniğin avantaj ve dezavantajları tartışılmıştır.

Anahtar Kelimeler

Gen Transferi, Agrobacterium, DNA enjeksiyonu

Kaynakça

• 1. De Cleene, M.,De Ley,J., The host range of crown gall. Bot. Rev. 42:389-466, 1976.
• 2. Draper, J., Scott, R., Armitage, P., Walden, R., Plant Genetic Transformation and Gene Expression: A Laboratory Manual. Blackwell Scientific Publ. Oxford, 1988.
• 3. Zambryski, P., Joos, H., Genetello, C., Leemans, J., Van Montagu, M.,Schell, J., Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 2:2143- 2150, 1983.
• 4. Hooykaas, P.J.J., Schilperoot, R.A., Agrobacterium and plant genetic engineering. Plant Mol.Biol. 19:25-38, 1992.
• 5. Stachel, S.E., Messens, E., Van Montagu, M., Zambryski, P., Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in A grobacterium tumefaciens. Nature 318:624-629, 1985.
• 6. Yanofsky, M.F., Porter, S.C., Young, C., Albright, L.M., Gordon, M.P., Nester, E.W., The virD operon of A grobacterium tumefaciens encodes a site-specific endonuclease. Cell 47:471-477, 1986.
• 7. Bevan, M.W., Flavell, R.B., Chilton, M-D., Chimaeric antibiotic resistance gene as a selectable marker for plant cell tranformation. Nature 304:184-186, 1983.
• 8. Jefferson, E.A., Kavanagh, T.A.,Bevan, M.W., GUS fusions: B-glucuronidase is a sensitive and versatile fusion marker in higher plants. EMBO J. 6:3901-3907, 1987.
• 9. Turgut, K., A study of anther gene function in Brassica napus using an antisense approach. Doktora Tezi, University of Leicester, Leicester, 1993.
• 10. Klein, T.M., Wolf, E.D., Wu, R.,Sanford, J.C., High- velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70-73, 1987.
• 11. Gordon-Kamm, W.J., Spencer, T.M., Mangano, M.L., Adams, T.R., Lemaux, P.G., Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2:603-618, 1990.
• 12. McCabe, D.E., Swain, W.F., Martinell, B.J., Christou, P., Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6:923-926, 1988.
• 13. Christou, P., Ford, T.L., Kofron, M., Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and Japonica varieties via electric dischardge acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9:957-962, 1991.
• 14. Paszkowski, J., Shillito, R.D., Saul, M., Mandak, V., Hohn, T., Hohn, B., Potrykus, I., Direct gene transfer to plants. EMBO J. 3:2717-2722, 1984.
• 15. Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J., Klein, T.M., Inheritance and expression of chimeric genes in progeny of transgenic maize plants. Bio/Technology 8:833-844, 1990.
• 16. Lorz, H., Baaker, B., Schell, J., Gene transfer to cereal cells mediates by protoplast transformation. Mol. Gen Genet. 199:183-188, 1985.
• 17. Potrykus, I., Saul, M.W., Paszkowski, J., Shillito, R.D., Direct gene transfer to cells of a graminaceous monocot. Mol. Gen. Genet. 199:183-188, 1985.
• 18. Zhang, H.M., Yang, H., Rech, E.L., Golds, T.J., Davis, S., Mulligan, B.J., Cocking, E.J., Davey, M.R., Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Rep. 7:379-384, 1988.
• 19. de La Pena, A., Lorz, H., Schell, J., Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature. 325:274-276, 1987.