DESELÜLERİZASYON YÖNTEMLERİ VE DOKULARDA KULLANIMI

Yıl 2017, Cilt: 2 Sayı: 2, 19 - 34, 29.12.2017

İbrahim Üçgül , Sultan Aras

Öz

Deselülerize
edilmiş doku ve organlar çeşitli doku mühendisliği ve rejeneratif tıp
uygulamalarında başarıyla kullanılmaktadır. Ekstraselüler matristen elde edilen
biyolojik bir iskele, işlem görecek dokudan hücreleri etkin bir şekilde ortadan
kaldıran çeşitli deselülerizasyon yöntemleri ile üretilebilir. Kullanılan deselülerizasyon
yöntemleri dokuların ve organların yapısına göre değişir. Hücrelerin bir
dokudan çıkarılması, dokunun kaynağına ve kullanılan spesifik fiziksel,
kimyasal ve enzimatik yöntemlere bağlıdır. Bu yöntemlerin her biri geri kalan
hücre dışı matrisin (ECM) yapısındaki biyokimyasal bileşimini, dokunun ince
yapısını  (ultrastrüktürünü) ve mekanik
davranışını etkiler. Bu makalede en sık kullanılan deselülerizasyon yöntemleri tanıtılarak
ve bu yöntemlerin biyolojik doku iskelesi materyali üzerindeki etkileri ele
alınmaktadır.

Anahtar Kelimeler

Deselülerizasyon, Ekstraselüler Matris, Doku mühendisliği (TE)

Kaynakça

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