Investigation of In Vitro Synergistic Effect of Niclosamide with Mupirocin in Staphylococcus aureus Strains by Screening Test

Öz

Increasing resistance to antibiotics used in Staphylococcus aureus carriers has brought up the search for alternative treatment. With this research it was aimed to examine whether niclosamide has an in vitro synergistic effect with mupirocin in S. aureus isolates. 25 methicillin-susceptible S. aureus (MSSA) and 25 methicillin-resistant S. aureus (MRSA) isolates were collected from the samples sent to our laboratory in 2021. In vitro synergistic effect between niclosamide and mupirocin was investigated by applying double disc synergy method in isolates. No synergy was detected between the two antimicrobial drugs in the isolates tested in the study. Mupirocin resistance was observed in only two of the isolates in the MRSA group, and all other isolates were found to be susceptible to mupirocin. No in vitro synergistic effect of niclosamide with mupirocin was observed in S. aureus isolates.

Anahtar Kelimeler

• mupirocin
• niclosamide
• Staphylococcus aureus

STAPHYLOCOCCUS AUREUS SUŞLARINDA NİKLOZAMİDİN MUPİROSİN İLE IN VİTRO SİNERJİSTİK ETKİSİNİN TARAMA TESTİ İLE ARAŞTIRILMASI

Öz

Staphylococcus aureus taşıyıcılarında kullanılan antibiyotiklere direncin artması alternatif tedavi arayışını gündeme getirmiştir. Bu araştırma ile S. aureus izolatlarında niklozamidin mupirosin ile in vitro sinerjistik etkisinin olup olmadığının incelenmesi hedeflenmiştir. 2021 yılında laboratuvarımıza gönderilen örneklerden 25 metisiline duyarlı S. aureus (MSSA) ve 25 metisiline dirençli S. aureus (MRSA) izolatı toplanmıştır. İzolatlarda çift disk sinerji yöntemi uygulanarak niklozamid ile mupirosin arasında in vitro sinerjistik etki olup olmadığı incelenmiştir. Çalışmada test edilen izolatlarda iki antimikrobiyal ilaç arasında herhangi bir sinerji saptanmamıştır. İzolatların sadece MRSA grubundaki iki tanesinde mupirosine direnç gözlenmiş olup, diğer izolatların tümünün mupirosine duyarlı olduğu bulunmuştur. S. aureus izolatlarında niklozamidin mupirosin ile in vitro sinerjistik etkisinin olmadığı gözlenmiştir.

Anahtar Kelimeler

• mupirosin
• niklozamid
• Staphylococcus aureus

Kaynakça

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