Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii)

Sema Coşkun; Hatice Dumanoğlu; Gölge Sarıkamış; Rabia Albayrak Delialioğlu; Zahide Kocabas

doi:10.15832/ankutbd.1661934

Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii)

Abstract

In this study, the objective was to establish an easier and more effective rooting and acclimatization protocol for the micropropagation of aronia compared to common in vitro and ex vitro methods. The effects of indole3-butyric acid (IBA) applied at concentrations of 0, 25, 50, 100, 200, 500, 1000 and 1500 mg/L on ex vitro rooting and acclimatization of 'Viking' and 'Nero' aronia cultivars (Aronia mitschurinii) were investigated. The microcuttings were quick-dipped in IBA solution and then placed in floating perlite. They were incubated for 4 weeks at 25±2 °C under a 16 hour light (35 μmol∙m-2∙s-1) and 8-hour dark photoperiod. The survival percentage of microcuttings decreased significantly (43.0-70.2%) at high concentrations of IBA including 500 mg/L and above, particularly in the ‘Viking’ cultivar. A second experiment was performed at lower concentrations including 25, 50, 100 and 200 mg/L IBA treatments and the control. The survival percentage of microcuttings was 97.7% and 94.6% and the rooting and acclimatization percentages of surviving microcuttings were 99.7% and 99.3% for ‘Viking’ and ‘Nero’ cultivars respectively without any significant difference among IBA treatments. To summarise, the experiment yielded successful results in the ex vitro rooting and simultaneous acclimatization of aronia microcuttings. These processes were achieved through the utilisation of a floating perlite bed, with and without low concentration of indole-3-butyric acid (IBA).

Keywords

Project Number

This research received no funding

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Details

Primary Language

English

Subjects

Horticultural Production (Other)

Journal Section

Research Article

Authors

Sema Coşkun This is me
0009-0001-3574-8217
Türkiye

Hatice Dumanoğlu ^*
0000-0002-7099-7630
Türkiye

Gölge Sarıkamış
0000-0003-0645-9464
Türkiye

Rabia Albayrak Delialioğlu
0000-0002-1969-4319
Türkiye

Zahide Kocabas
0000-0002-0310-4062
Türkiye

Publication Date

January 20, 2026

Submission Date

March 20, 2025

Acceptance Date

June 27, 2025

Published in Issue

Year 2026 Volume: 32 Number: 1

DOI

https://doi.org/10.15832/ankutbd.1661934

IZ

https://izlik.org/JA63ZR23RA

Cite

RIS / Bibtex

APA

Coşkun, S., Dumanoğlu, H., Sarıkamış, G., Albayrak Delialioğlu, R., & Kocabas, Z. (2026). Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii). Journal of Agricultural Sciences, 32(1), 81-92. https://doi.org/10.15832/ankutbd.1661934

AMA

1.Coşkun S, Dumanoğlu H, Sarıkamış G, Albayrak Delialioğlu R, Kocabas Z. Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii). J Agr Sci-Tarim Bili. 2026;32(1):81-92. doi:10.15832/ankutbd.1661934

Chicago

Coşkun, Sema, Hatice Dumanoğlu, Gölge Sarıkamış, Rabia Albayrak Delialioğlu, and Zahide Kocabas. 2026. “Improvement of Ex Vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia Mitschurinii)”. Journal of Agricultural Sciences 32 (1): 81-92. https://doi.org/10.15832/ankutbd.1661934.

EndNote

Coşkun S, Dumanoğlu H, Sarıkamış G, Albayrak Delialioğlu R, Kocabas Z (January 1, 2026) Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii). Journal of Agricultural Sciences 32 1 81–92.

IEEE

[1]S. Coşkun, H. Dumanoğlu, G. Sarıkamış, R. Albayrak Delialioğlu, and Z. Kocabas, “Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii)”, J Agr Sci-Tarim Bili, vol. 32, no. 1, pp. 81–92, Jan. 2026, doi: 10.15832/ankutbd.1661934.

ISNAD

Coşkun, Sema - Dumanoğlu, Hatice - Sarıkamış, Gölge - Albayrak Delialioğlu, Rabia - Kocabas, Zahide. “Improvement of Ex Vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia Mitschurinii)”. Journal of Agricultural Sciences 32/1 (January 1, 2026): 81-92. https://doi.org/10.15832/ankutbd.1661934.

JAMA

1.Coşkun S, Dumanoğlu H, Sarıkamış G, Albayrak Delialioğlu R, Kocabas Z. Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii). J Agr Sci-Tarim Bili. 2026;32:81–92.

MLA

Coşkun, Sema, et al. “Improvement of Ex Vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia Mitschurinii)”. Journal of Agricultural Sciences, vol. 32, no. 1, Jan. 2026, pp. 81-92, doi:10.15832/ankutbd.1661934.

Vancouver

1.Sema Coşkun, Hatice Dumanoğlu, Gölge Sarıkamış, Rabia Albayrak Delialioğlu, Zahide Kocabas. Improvement of Ex vitro Rooting and Acclimatization to Increase Micropropagation Efficiency of Aronia (Aronia mitschurinii). J Agr Sci-Tarim Bili. 2026 Jan. 1;32(1):81-92. doi:10.15832/ankutbd.1661934