Phenolic Composition and Antioxidant Activities of Wines and Extracts of Some Grape Varieties Grown in Turkey
Year 2011,
Volume: 17 Issue: 1, 67 - 76, 06.03.2011
Nilgün Baydar
Zehra Babalık
,
Filiz Türk
Emine Çetin
Abstract
Grape seed and skin extracts and wines from Cabernet Sauvignon, Kalecik Karası and Narince grape cultivars were
assayed for their antioxidant properties and phenolic compositions. Total phenolic contents of the samples were
determined by the Folin Ciocalteu method and compositions of the phenolics were separated by HPLC. Antioxidant
activities of the samples were evaluated using the 1, 1-diphenyl-2- picrylhydrazyl (DPPH.
) radical scavenging and
reducing power methods. Total phenolic contents varied from 522.49 to 546.50 mg GAE g-1 in seed extracts; from
22.73 to 43.75 mg GAE g-1 in skin extracts and from 217.06 to 1336.21 GAE mg l
-1 in wines. Radical scavenging
activities and reducing powers of the samples changed depending on the grape cultivars and the different parts of
grape and wine types.
References
- Bakkalbaşı E, Yemis O¸ Aslanova D & Artık N (2005).
Major flavan-3-ol composition and antioxidant
activity of seeds from different grape cultivars grown
in Turkey. European Food Research Technology
221: 792-797
- Baumann J, Wurm G & Bruchhausen F (1979).
Prostaglandin synthetase inhibiting O2-radical
scavenging properties of some flavonoids and related
phenolic compounds. Naunyn-Schmiedebergs
Archives of Pharmacology 30: 27-32
- Baydar N G, Özkan G & Yaşar S (2007). Evaluation of
the antiradical and antioxidant potential of grape
extracts. Food Control 18: 1131-1136
- Baydar N G (2006). Organic acids, tocopherols and
phenolic compositions of some Turkish grape
cultivars. Chemistry of Natural Compounds 42(2):
156-159
- Baydar N G, Sağdıç O, Özkan G & Çetin E S (2006).
Determination of antibacterial effects and total
phenolic contents of grape (Vitis vinifera L.) seed
extracts. International Journal of Food Science and
Technology 41: 799-804
- Caponio F, Alloggio V & Gomes T (1999). Phenolic
compounds of virgin olive oil:Influence of paste
preparation techniques. Food Chemistry 64: 203-209
Careri M, Corradini C, Elviri L, Nicoletti L & Zagnoni I
(2003). Direct HPLC analysis of quercetin and transresveratrol in red wine, grape, and winemaking
byproducts. Journal of Agricultural Food Chemistry
51: 5226-5231
- Crozier A. Jaganath I B & Clifford M N (2007). Phenols,
Polyphenols and Tannins: An Overview. In Plant
Secondary Metabolites, edited by M. N. C. H. A.
Alan Crozier.
- Gadow A, Joubert E & Hansmann C F (1997).
Comparasion of the antioxidant activity of aspalathin
with that of other plant phenols of rooibos tea
(Aspalathus linearis), α-tocopherol, BHT, and BHA.
Journal of Agricultural Food Chemistry 45: 632-638
- Gomez-Alonso S, Garcia Romero E & Hermosin
Gutierrez I (2007). HPLC analysis of diverse grape
and wine phenolics using direct injection and
multidetection by DAD and fluorescence. Journal of
Food Composition and Analysis 20: 618-626
- Iacopini P, Baldi M, Storchi P & Sebastiani L (2008).
Catechin, epicatechin, quercetin, rutin and resveratrol
in red grape: Content, in vitro antioxidant activity and
interactions. Journal of Food Composition and
Analysis 21:589-598
- Ito N, Hiroze M, Fukushima G, Tauda H, Shira T &
Tatematsu M (1986). Studies on antioxidant; their
carcinogenic and modifying effects on chemical
carcinogenesis. Food Chemistry Toxicology 24:
1071-1081
- Jayaprakasha G K, Selvi T & Sakariah K K (2003).
Antibacterial and antioxidant activities of grape (Vitis
vinifera) seed extracts. Food Research International
36: 117-122
- Jayaprakasha G K, Signh R P & Sakariah K K (2001).
Antioxidant activity of grape seed (Vitis vinifera)
extracts on peroxidation models in vitro. Food
Chemistry 73: 285-290
- Katalinic V, Milos M, Modun D, Music I & Boban M
(2004). Antioxidant effectiveness of selected wines in
comparison with (+)-catechin. Food Chemistry 86:
593-600
- Kelebek H, Canbaş A, Jourdes, M & Teissedre P L
(2010a). Characterization of colored and colorless
phenolic compounds in Öküzgözü wines from
Denizli and Elazig regions using HPLC-DAD–MS.
Industrial Crops and Products 31: 499-508
- Kelebek H, Selli S, & Canbaş A (2010b). Öküzgözü
üzümlerinden kırmızı şarap üretiminde soğuk
maserasyon uygulamasının antosiyaninler üzerine
etkisi. Tarım Bilimleri Dergisi 16(4): 287-294
- Kinsella J E, Frankel E, German B & Kanner J (1993).
Possible mechanisms for the protective role of
antioxidants in wine and plant foods. Food
Technology 47: 85-89
- Lante A, Crapisi A, Lomolino G & Spettoli P (2004).
Chemical parameters, biologically active polyphenols
and sensory characteristics of some Italian organic
wines. Journal of Wine Research 15(3): 203-209
- Li H, Wang X, Li Y, Li P & Wang H (2009).
Polyphenolic compounds and antioxidant properties
of selected China wines. Food Chemistry 112: 454-
460
- Lopez M, Martinez F, Del Valle C, Orte C & Miro M
(2001). Analysis of phenolics constituents of
biological interest in red wines by HPLC. Journal of
Chromatography 922: 359-363
- Makris D P, Kallithraka S, Kefalas P (2006). Flavonols
in grapes, grape products and wines: Burden, profile
and influential parameters. Journal of Food
Composition and Analysis 19: 396-404
- Murthy K N C, Singh R P & Jayaprakasha G K (2002).
Antioxidant activity of grape (Vitis vinifera) pomace
extracts. Journal of Agricultural and Food Chemistry
50: 5909-5914
- Oyaizu M (1986). Studies on products of browning
reaction: antioxidative activities of products of
browning reaction prepared from glucosamine.
Japanese Journal of Nutrition 44: 307-315
- Özkan G & Baydar N G (2006). A direct RP-HPLC
determination of phenolic compounds in Turkish red
wines. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi
19(2): 229-234
- Proestos C, Bakogiannis A, Psarianos C, Koutinas A A,
Kanellaki M & Komaitis M (2005). High
performance liquid chromatography analyses of
phenolic substances in Greek Wines. Food Control
16: 319-323
- Revilla E & Ryan J M (2000). Analysis of several
phenolic compounds with potential antioxidant
properties in grape extracts and wines by HPLCphotodiode array detection without sample
preparation. Journal of Chromatography A 881: 461-
469
- Ricardo da Silva J M, Rosec J P, Bourzeix M & Heredia
N (1990). Separation and quantitative determination
of grape and wine procyanidins by HPLC. Journal of
the Science of Food and Agriculture 53: 85–92
- Rice Evans C A, Miller N J & Paganga G (1996).
Structure antioxidant activity relationship of
flavonoids and phenolic acids. Free Radical Biology
and Medicine 20: 933-956
- Rockenbach I V, Gonzaga L V, Rizelio V M, Schmidt
Gonçalves A E S, Genovese M I & Fett R (2011)
Phenolic compounds and antioxidant activity of seed
and skin extracts of red grape (Vitis vinifera and Vitis
labrusca) pomace from Brazilian winemaking. Food
Research International 44(4): 897-901
- Shadidi F & Naczk M (1995). Food Phenols: Source,
Chemistry, Effects and Applications. Technomic
Publishing Company (USA). pp. 331
- Sherwin E R (1978). Oxidation and antioxidants in fat
and oil processing. Journal of American Oil
Chemical Society 55: 809-814
- Shimada K, Fujikawa K, Yahara K & Nakamura T
(1992). Antioxidative properties of xanthan on the
autoxidation of soybean oil in cyclodextrin emulsion.
Journal of Agricultural and Food Chemistry 40: 945-
948
- Singleton VL & Rossi J R (1965). Colorimetry of total
phenolics with Phospho molybdic-phosphotungstic
acid. American Journal of Enology and Viticulture
16: 144-158
- Sun J, Chu Y F, Wu X & Liu R H (2002). Antioxidant
and antiproliferative activities of common fruits.
Journal of Agricultural Food Chemistry 50: 7449-
7454
- Yu J Vasanthan T & Temelli F (2001). Analysis of
phenolic acids in barley by high performance liquid
chromatography. Journal of Agricultural Food
Chemistry 49: 4352-4358
Üzüm ekstraktları ve şarapların fenolik kompozisyonları ve antioksidan aktiviteleri
Year 2011,
Volume: 17 Issue: 1, 67 - 76, 06.03.2011
Nilgün Baydar
Zehra Babalık
,
Filiz Türk
Emine Çetin
Abstract
Bu araştırmada Cabernet Sauvignon, Kalecik Karası ve Narince üzüm çeşitlerine ait üzüm çekirdeği ve kabuk ekstraktları ile şarapların antioksidan özellikleri ile fenolik bileşik içerikleri tespit edilmiştir. Toplam fenolik içeriği Folin Ciocalteu metodu ile; fenolik bileşiklerin tanımlanması ise HPLC de gerçekleştirilmiştir. Antioksidan aktivite 1,1-difenil -2- pikrilhidrazil (DPPH) radikal süpürme etkisi ve indirgeme kapasitesi metodu ile belirlenmiştir. Toplam fenolik içeriğinin çekirdek ekstraktlarında 522.49 ile 546.50 mg GAE g-1; kabuk ekstraktlarında 22.73 ile 43.75 mg GAE g-1 ve şaraplarda 217.06 ile 1336.21 mg L-1 arasında değiştiği belirlenmiştir. Örneklerin radikal süpürme etkilerinin ve indirgeme kapasiteleri üzüm çeşitlerine, üzümün kısımlarına ve şarabın tipine bağlı olarak değişiklik göstermiştir.
References
- Bakkalbaşı E, Yemis O¸ Aslanova D & Artık N (2005).
Major flavan-3-ol composition and antioxidant
activity of seeds from different grape cultivars grown
in Turkey. European Food Research Technology
221: 792-797
- Baumann J, Wurm G & Bruchhausen F (1979).
Prostaglandin synthetase inhibiting O2-radical
scavenging properties of some flavonoids and related
phenolic compounds. Naunyn-Schmiedebergs
Archives of Pharmacology 30: 27-32
- Baydar N G, Özkan G & Yaşar S (2007). Evaluation of
the antiradical and antioxidant potential of grape
extracts. Food Control 18: 1131-1136
- Baydar N G (2006). Organic acids, tocopherols and
phenolic compositions of some Turkish grape
cultivars. Chemistry of Natural Compounds 42(2):
156-159
- Baydar N G, Sağdıç O, Özkan G & Çetin E S (2006).
Determination of antibacterial effects and total
phenolic contents of grape (Vitis vinifera L.) seed
extracts. International Journal of Food Science and
Technology 41: 799-804
- Caponio F, Alloggio V & Gomes T (1999). Phenolic
compounds of virgin olive oil:Influence of paste
preparation techniques. Food Chemistry 64: 203-209
Careri M, Corradini C, Elviri L, Nicoletti L & Zagnoni I
(2003). Direct HPLC analysis of quercetin and transresveratrol in red wine, grape, and winemaking
byproducts. Journal of Agricultural Food Chemistry
51: 5226-5231
- Crozier A. Jaganath I B & Clifford M N (2007). Phenols,
Polyphenols and Tannins: An Overview. In Plant
Secondary Metabolites, edited by M. N. C. H. A.
Alan Crozier.
- Gadow A, Joubert E & Hansmann C F (1997).
Comparasion of the antioxidant activity of aspalathin
with that of other plant phenols of rooibos tea
(Aspalathus linearis), α-tocopherol, BHT, and BHA.
Journal of Agricultural Food Chemistry 45: 632-638
- Gomez-Alonso S, Garcia Romero E & Hermosin
Gutierrez I (2007). HPLC analysis of diverse grape
and wine phenolics using direct injection and
multidetection by DAD and fluorescence. Journal of
Food Composition and Analysis 20: 618-626
- Iacopini P, Baldi M, Storchi P & Sebastiani L (2008).
Catechin, epicatechin, quercetin, rutin and resveratrol
in red grape: Content, in vitro antioxidant activity and
interactions. Journal of Food Composition and
Analysis 21:589-598
- Ito N, Hiroze M, Fukushima G, Tauda H, Shira T &
Tatematsu M (1986). Studies on antioxidant; their
carcinogenic and modifying effects on chemical
carcinogenesis. Food Chemistry Toxicology 24:
1071-1081
- Jayaprakasha G K, Selvi T & Sakariah K K (2003).
Antibacterial and antioxidant activities of grape (Vitis
vinifera) seed extracts. Food Research International
36: 117-122
- Jayaprakasha G K, Signh R P & Sakariah K K (2001).
Antioxidant activity of grape seed (Vitis vinifera)
extracts on peroxidation models in vitro. Food
Chemistry 73: 285-290
- Katalinic V, Milos M, Modun D, Music I & Boban M
(2004). Antioxidant effectiveness of selected wines in
comparison with (+)-catechin. Food Chemistry 86:
593-600
- Kelebek H, Canbaş A, Jourdes, M & Teissedre P L
(2010a). Characterization of colored and colorless
phenolic compounds in Öküzgözü wines from
Denizli and Elazig regions using HPLC-DAD–MS.
Industrial Crops and Products 31: 499-508
- Kelebek H, Selli S, & Canbaş A (2010b). Öküzgözü
üzümlerinden kırmızı şarap üretiminde soğuk
maserasyon uygulamasının antosiyaninler üzerine
etkisi. Tarım Bilimleri Dergisi 16(4): 287-294
- Kinsella J E, Frankel E, German B & Kanner J (1993).
Possible mechanisms for the protective role of
antioxidants in wine and plant foods. Food
Technology 47: 85-89
- Lante A, Crapisi A, Lomolino G & Spettoli P (2004).
Chemical parameters, biologically active polyphenols
and sensory characteristics of some Italian organic
wines. Journal of Wine Research 15(3): 203-209
- Li H, Wang X, Li Y, Li P & Wang H (2009).
Polyphenolic compounds and antioxidant properties
of selected China wines. Food Chemistry 112: 454-
460
- Lopez M, Martinez F, Del Valle C, Orte C & Miro M
(2001). Analysis of phenolics constituents of
biological interest in red wines by HPLC. Journal of
Chromatography 922: 359-363
- Makris D P, Kallithraka S, Kefalas P (2006). Flavonols
in grapes, grape products and wines: Burden, profile
and influential parameters. Journal of Food
Composition and Analysis 19: 396-404
- Murthy K N C, Singh R P & Jayaprakasha G K (2002).
Antioxidant activity of grape (Vitis vinifera) pomace
extracts. Journal of Agricultural and Food Chemistry
50: 5909-5914
- Oyaizu M (1986). Studies on products of browning
reaction: antioxidative activities of products of
browning reaction prepared from glucosamine.
Japanese Journal of Nutrition 44: 307-315
- Özkan G & Baydar N G (2006). A direct RP-HPLC
determination of phenolic compounds in Turkish red
wines. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi
19(2): 229-234
- Proestos C, Bakogiannis A, Psarianos C, Koutinas A A,
Kanellaki M & Komaitis M (2005). High
performance liquid chromatography analyses of
phenolic substances in Greek Wines. Food Control
16: 319-323
- Revilla E & Ryan J M (2000). Analysis of several
phenolic compounds with potential antioxidant
properties in grape extracts and wines by HPLCphotodiode array detection without sample
preparation. Journal of Chromatography A 881: 461-
469
- Ricardo da Silva J M, Rosec J P, Bourzeix M & Heredia
N (1990). Separation and quantitative determination
of grape and wine procyanidins by HPLC. Journal of
the Science of Food and Agriculture 53: 85–92
- Rice Evans C A, Miller N J & Paganga G (1996).
Structure antioxidant activity relationship of
flavonoids and phenolic acids. Free Radical Biology
and Medicine 20: 933-956
- Rockenbach I V, Gonzaga L V, Rizelio V M, Schmidt
Gonçalves A E S, Genovese M I & Fett R (2011)
Phenolic compounds and antioxidant activity of seed
and skin extracts of red grape (Vitis vinifera and Vitis
labrusca) pomace from Brazilian winemaking. Food
Research International 44(4): 897-901
- Shadidi F & Naczk M (1995). Food Phenols: Source,
Chemistry, Effects and Applications. Technomic
Publishing Company (USA). pp. 331
- Sherwin E R (1978). Oxidation and antioxidants in fat
and oil processing. Journal of American Oil
Chemical Society 55: 809-814
- Shimada K, Fujikawa K, Yahara K & Nakamura T
(1992). Antioxidative properties of xanthan on the
autoxidation of soybean oil in cyclodextrin emulsion.
Journal of Agricultural and Food Chemistry 40: 945-
948
- Singleton VL & Rossi J R (1965). Colorimetry of total
phenolics with Phospho molybdic-phosphotungstic
acid. American Journal of Enology and Viticulture
16: 144-158
- Sun J, Chu Y F, Wu X & Liu R H (2002). Antioxidant
and antiproliferative activities of common fruits.
Journal of Agricultural Food Chemistry 50: 7449-
7454
- Yu J Vasanthan T & Temelli F (2001). Analysis of
phenolic acids in barley by high performance liquid
chromatography. Journal of Agricultural Food
Chemistry 49: 4352-4358