Domates Bitkisinde Kurşun Pb ve Kadminyumun Cd Lipid Peroksidasyonu, Katalaz CAT Enzim Aktivitesi ve Gen Ekpresyon Profiline Etkisi

Year 2016, Volume: 22 Issue: 4, 539 - 547, 01.09.2016

Semra Soydam Aydın İlker Büyük Esra Gökçe Gündüzer Burcu Pelin Büyük İrfan Kandemir Demet Cansaran-duman Sümer Aras

https://doi.org/10.1501/Tarimbil_0000001411

Abstract

Önemli bir abiyotik stres faktörü olan ağır metaller bitkilerde çok çeşitli yanıt mekanizmalarını uyarabilirler. Bu yanıt mekanizmaları; membran kompozisyonunda değişiklik, küçük molekül ve serbest radikallerin üretimi, antioksidant enzimlerin aktivitelerinin ya da gen ekspresyonlarının değişimini içerir. Bu sebeple, bu araştırmada çeşitli konsantrasyonlardaki 0, 80, 160, 320, 640 ve 1280 μM Pb2+ve Cd2+ kontaminasyonuna maruz kalan domates bitkilerinde lipid peroksidasyon seviyesi MDA , katalaz enzim aktivitesi ve real-time PCR aracılığı ile katalaz gen ekpresyon seviyesi belirlenmiştir. 320 ve 640 μM Cd+2 kontaminasyonu hariç tüm Cd+2 ve Pb+2 kontaminasyonları lipid peroksidasyonuna ve katalaz enzim aktivitesinde artışa neden olmuştur. Sonuç olarak; çeşitli konsantrasyonlarda Pb2+ ve Cd2+ kontaminasyonuna maruz kalan domates bitkisinde, CAT gen ekpresyonu ve lipid peroksidayonu arasında pozitif korelasyon bulunurken, CAT gen ekpresyonu ve enzim aktivitesi arasında korelasyon tespit edilememiştir. Bu durum genlerin transkripsiyonel, posttranskripsiyonel ve aynı zamanda translasyonel veya posttranslasyonel seviyelerdeki regülasyonu ile açıklanabilir

Keywords

Domates Solanum lycopersicum L., Lipid peroksidasyonu, CAT enzim aktivitesi, Gen ekpresyonu, Real-time PCR

Effects of Lead Pb and Cadmium Cd Elements on Lipid Peroxidation, Catalase Enzyme Activity and Catalase Gene Expression Profile in Tomato Plants

Abstract

Heavy metals are significant abiotic stress factor, affecting various response mechanisms in plants. These responses include: changes in membrane composition, production of small molecules and free radicals, and alterations in the activities of antioxidant enzymes and their gene expressions. For this reason, lipid peroxidation levels MDA , catalase enzyme activity, and gene expression profiles, quantified by real-time PCR, were analyzed in tomato plants exposed to various concentrations 0, 80, 160, 320, 640 and 1280 μM of Cd2+ and Pb2+. All concentration of Cd+2 or Pb+2 contamination led to increased lipid peroxidation and catalase enzyme activity, except for 320 and 640 μM Cd+2 contamination levels. As a result, gene expression patterns at the mRNA level and changes in MDA content under different concentrations of Pb+2 and Cd+2 contamination revealed a positive correlation, although no correlation was found between gene expression patterns at the mRNA level and catalase enzyme activity. These results might be explained by the regulation of genes at the transcriptional, posttranscriptional, and also translational or posttranslational levels

Keywords

Tomato Solanum lycopersicum L., Lipid peroxidation, CAT enzyme activity, Gene expression, qRT-PCR

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