The Effect of UV-A / UV-B Radiation on Quality Changes of Harvested Curly Lettuce During the Storage
Year 2025,
Volume: 31 Issue: 1, 80 - 90, 14.01.2025
Öznur Cumhur Değirmenci
,
Alev Akpinar Borazan
,
Emre Devlez
Abstract
This study investigated the effects of UV-A and UV-B radiation on curly lettuce quality. Results focused on colour, total phenolic content, antioxidant activity, and ascorbic acid. The findings revealed that the highest phenolic content (46.1 mg GAE/100 g FL) had been observed in lettuce samples treated with high dose UV-B on the 7th day. The lowest phenolic content (13.7 mg GAE/100 g FL) was recorded in those treated with low dose UV-B on the same day of storage. Data showed an increase of 29.7% in antioxidant activity and 53.7% in total phenolic content after 7 days of storage in samples treated with high dose UV-B. High dose UV-A radiation was found to be the most effective in maintaining and enhancing the ascorbic acid content of the lettuce. UV applications did not cause yellowing in the stored lettuce leaves. Further research on different doses and optimization is recommended.
Ethical Statement
This is not a research that requires ethics committee approval.
Supporting Institution
Bilecik Şeyh Edebali University
Project Number
Research Fund of Bilecik Şeyh Edebali University (BAP project-2020-02.BŞEÜ.25-04)
Thanks
The authors appreciate the support from Biotechnology Application and Research Center at Bilecik Şeyh Edebali University.
References
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- Aiamla-or S, Yamauchi N, Takino S & Shigyo M (2009). Effect of UV-A and UV-B irradiation on broccoli (Brassica oleracea L. Italica Group) floret yellowing during storage. Postharvest Biology and Technology 54(3): 177–179. https://doi.org/10.1016/J.POSTHARVBIO.2009.07.006
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- Azarafshan M, Peyvandi M, Abbaspour H, Noormohammadi Z & Majd A (2020). The effects of UV-B radiation on genetic and biochemical changes of Pelargonium graveolens L'Her. Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology 26(3): 605–616. https://doi.org/10.1007/s12298-020-00758-6
- Castagna A, Chiavaro E, Dall’Asta C, Rinaldi M, Galaverna G & Ranieri A (2013). Effect of postharvest UV-B irradiation on nutraceutical quality and physical properties of tomato fruits. Food Chemistry 137(1–4): 151–158. https://doi.org/10.1016/J.FOODCHEM.2012.09.095
- Cefola M & Pace B (2023). Advances postharvest preservation technology. Foods 12(8): 1664. https://doi.org/10.3390/foods12081664
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- Das R & Bhattacharjee C (2020). Lettuce. In A. K. Jaiswal (Ed.). Nutritional composition and antioxidant properties of fruits and vegetables. United Kingdom: Academic Press. p. 143–157.
- Du WX, Avena-Bustillos RJ, Breksa AP & McHugh TH (2014). UV-B light as a factor affecting total soluble phenolic contents of various whole and fresh-cut specialty crops. Postharvest Biology and Technology 93: 72–82. https://doi.org/10.1016/J.POSTHARVBIO.2014.02.004
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Jansen MAK, Hectors K, O’Brien NM, Guisez Y & Potters G (2008). Plant stress and human health: Do human consumers benefit from UV-B acclimated crops? Plant Science 175(4): 449–458. https://doi.org/10.1016/J.PLANTSCI.2008.04.010
- Kang HM & Saltveit ME (2002). Antioxidant capacity of lettuce leaf tissue increases after wounding. Journal of Agricultural and Food Chemistry 50(26): 7536–7541. https://doi.org/10.1021/JF020721C
- Kasim MU & Kasim R (2017a). The effects of ultraviolet B (UV-B) irradiation on color quality and decay rate of Capia pepper during postharvest storage. Food Science and Technology International 38(2): 363–368. https://doi.org/10.1590/1678-457X.05817
- Kasim MU & Kasim R (2017b). Yellowing of fresh-cut spinach (Spinacia oleracea L.) leaves delayed by UV-B applications. Information Processing in Agriculture 4(3): 214–219. https://doi.org/10.1016/J.INPA.2017.05.006
- Kim MJ, Moon Y, Tou JC, Mou B & Waterland NL (2016). Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.). Journal of Food Composition and Analysis 49: 19–34. https://doi.org/10.1016/J.JFCA.2016.03.004
- Koutchma T (2009). Advances in ultraviolet light technology for non-thermal processing of liquid foods. Food and Bioprocess Technology 2(2): 138–155. https://doi.org/10.1007/S11947-008-0178-3/FIGURES/3
Koutchma T (2019). Ultraviolet light in food technology: Principles and applications. New york: CRC Press. 337 p.
- Liu C, Han X, Cai L, Lu X, Ying T & Jia Z (2011). Postharvest UV-B irradiation maintains sensory qualities and enhances antioxidant capacity in tomato fruit during storage. Postharvest Biology and Technology 59(3): 232–237. https://doi.org/10.1016/J.POSTHARVBIO.2010.09.003
- Liu X, Ardo S, Bunning M, Parry J, Zhou K, Stushnoff C, Stoniker F, Yu L & Kendall P (2007). Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT-Food Science and Technology 40(3): 552–557. https://doi.org/10.1016/J.LWT.2005.09.007
- Llorach R, Tomás-Barberán FA & Ferreres F (2004). Lettuce and chicory by products as a source of antioxidant phenolic extracts. Journal of Agricultural and Food Chemistry 52(16): 5109–5116. https://doi.org/10.1021/JF040055A
- Mampholo BM, Maboko MM, Soundy P & Sivakumar D (2016). Phytochemicals and overall quality of leafy lettuce (Lactuca sativa L.) varieties grown in closed hydroponic system. Journal of Food Quality 39(6): 805–815. https://doi.org/10.1111/JFQ.12234
- Martínez-Ispizua E, Calatayud Á, Marsal JI, Basile F, Cannata C, Abdelkhalik A, Soler S, Valcárcel JV & Martínez-Cuenca MR (2022). Postharvest changes in the nutritional properties of commercial and traditional lettuce varieties in relation with overall visual quality. Agronomy 12(2): 403. https://doi.org/10.3390/AGRONOMY12020403/S1
- Muscolo A, Marra F, Canino F, Maffia A, Mallamaci C & Russo M (2022). Growth, nutritional quality and antioxidant capacity of lettuce grown on two different soils with sulphur-based fertilizer, organic and chemical fertilizers. Scientia Horticulturae 305: 111421. https://doi.org/10.1016/J.SCIENTA.2022.111421
- Nadkarni BY (1965). Determination of ascorbic acid in coloured extracts: A new modification of the indophenol technique. Mikrochimica Acta 53(1): 21–27. https://doi.org/10.1007/BF01218730/METRICS
- Nguyen CTT, Kim J, Yoo KS, Lim S & Lee EJ (2014). Effect of prestorage UV-A, -B, and -C radiation on fruit quality and anthocyanin of “Duke” blueberries during cold storage. Journal of Agricultural and Food Chemistry 62(50): 12144–12151. https://doi.org/10.1021/JF504366X/ASSET/IMAGES/MEDIUM/JF-2014-04366X_0005.GIF
- Nicolle C, Carnat A, Fraisse D, Lamaison JL, Rock E, Michel H, Amoureux P & Remesy C (2004). Characterisation and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). Journal of the Science of Food and Agriculture 84(15): 2061–2069. https://doi.org/10.1002/JSFA.1916
- Pathare PB, Opara UL & Al-Said FAj (2013). Colour measurement and analysis in fresh and processed foods: A review. Food Bioprocess Technology 6: 36–60. https://doi.org/10.1007/s11947-012-0867-9
- Paul ND, Moore JP, McPherson M, Lambourne C, Croft P, Heaton JC & Wargent JJ (2012). Ecological responses to UV radiation: Interactions between the biological effects of UV on plants and on associated organisms. Physiologia Plantarum 145(4): 565–581. https://doi:10.1111/j.1399-3054.2011.01553.x
- Pérez-López U, Miranda-Apodaca J, Muñoz-Rueda A & Mena-Petite A (2013). Lettuce production and antioxidant capacity are differentially modified by salt stress and light intensity under ambient and elevated CO2. Journal of Plant Physiology 170(17): 1517–1525. https://doi.org/10.1016/J.JPLPH.2013.06.004
- Salemi S, Saedisomeolia A, Azimi F, Zolfigol S, Mohajerani E, Mohammadi M & Yaseri M (2021). Optimizing the production of vitamin D in white button mushrooms (Agaricus bisporus) using ultraviolet radiation and measurement of its stability. Lebensmittel-Wissenschaft + [i.e. Und] Technologie. Food Science + Technology. Science + Technologie Alimentaire 137: 110401. https://doi.org/10.1016/J.LWT.2020.110401
- Scattino C, Negrini N, Morgutti S, Cocucci M, Crisosto CH, Tonutti P, Castagna A & Ranieri A (2016). Cell wall metabolism of peaches and nectarines treated with UV-B radiation: A biochemical and molecular approach. Journal of the Science of Food and Agriculture 96(3): 939–947. https://doi.org/10.1002/JSFA.7168
- Tikekar RV, Anantheswaran RC, Elias RJ & Laborde LF (2011). Ultraviolet-induced oxidation of ascorbic acid in a model juice system: Identification of degradation products. Journal of Agricultural and Food Chemistry 59(15): 8244–8248. https://doi.org/10.1021/JF201000X
- Tomás-Callejas A, Otón M, Artés F & Artés-Hernández F (2012). Combined effect of UV-C pretreatment and high oxygen packaging for keeping the quality of fresh-cut Tatsoi baby leaves. Innovative Food Science & Emerging Technologies 14: 115–121. https://doi.org/10.1016/J.IFSET.2011.11.007
- Usall J, Ippolito A, Sisquella M & Neri F (2016). Physical treatments to control postharvest diseases of fresh fruits and vegetables. Postharvest Biology and Technology 122: 30–40. https://doi.org/10.1016/J.POSTHARVBIO.2016.05.002
- Wang W, Zhang C, Shang M, Lv H, Liang B, Li J & Zhou W (2022). Hydrogen peroxide regulates the biosynthesis of phenolic compounds and antioxidant quality enhancement in lettuce under low nitrogen condition. Food Chemistry: X 16: 100481. https://doi.org/10.1016/J.FOCHX.2022.100481
Year 2025,
Volume: 31 Issue: 1, 80 - 90, 14.01.2025
Öznur Cumhur Değirmenci
,
Alev Akpinar Borazan
,
Emre Devlez
Project Number
Research Fund of Bilecik Şeyh Edebali University (BAP project-2020-02.BŞEÜ.25-04)
References
- Aiamla-or S, Kaewsuksaeng S, Shigyo M & Yamauchi N (2010). Impact of UV-B irradiation on chlorophyll degradation and chlorophyll-degrading enzyme activities in stored broccoli (Brassica oleracea L. Italica Group) florets. Food Chemistry 120(3): 645–651. https://doi.org/10.1016/J.FOODCHEM.2009.10.056
- Aiamla-or S, Yamauchi N, Takino S & Shigyo M (2009). Effect of UV-A and UV-B irradiation on broccoli (Brassica oleracea L. Italica Group) floret yellowing during storage. Postharvest Biology and Technology 54(3): 177–179. https://doi.org/10.1016/J.POSTHARVBIO.2009.07.006
- Allende A, McEvoy JL, Luo Y, Artes F & Wang CY (2006). Effectiveness of two-sided UV-C treatments in inhibiting natural microflora and extending the shelf-life of minimally processed “Red Oak Leaf” lettuce. Food Microbiology 23(3): 241–249. https://doi.org/10.1016/J.FM.2005.04.009
- Azarafshan M, Peyvandi M, Abbaspour H, Noormohammadi Z & Majd A (2020). The effects of UV-B radiation on genetic and biochemical changes of Pelargonium graveolens L'Her. Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology 26(3): 605–616. https://doi.org/10.1007/s12298-020-00758-6
- Castagna A, Chiavaro E, Dall’Asta C, Rinaldi M, Galaverna G & Ranieri A (2013). Effect of postharvest UV-B irradiation on nutraceutical quality and physical properties of tomato fruits. Food Chemistry 137(1–4): 151–158. https://doi.org/10.1016/J.FOODCHEM.2012.09.095
- Cefola M & Pace B (2023). Advances postharvest preservation technology. Foods 12(8): 1664. https://doi.org/10.3390/foods12081664
- Collado-González J, Piñero MC, Otalora G, Lopez-Marín J & del Amor FM (2022). Unraveling the nutritional and bioactive constituents in baby-leaf lettuce for challenging climate conditions. Food Chemistry 384: 132506. https://doi.org/10.1016/J.FOODCHEM.2022.132506
- Csapó J, Prokisch J, Albert CS & Sipos P (2019). Effect of UV light on food quality and safety. Acta Universitatis Sapientiae, Alimentaria 12(2019): 21-41. https://doi.org/10.2478/ausal-2019-0002
- Das R & Bhattacharjee C (2020). Lettuce. In A. K. Jaiswal (Ed.). Nutritional composition and antioxidant properties of fruits and vegetables. United Kingdom: Academic Press. p. 143–157.
- Du WX, Avena-Bustillos RJ, Breksa AP & McHugh TH (2014). UV-B light as a factor affecting total soluble phenolic contents of various whole and fresh-cut specialty crops. Postharvest Biology and Technology 93: 72–82. https://doi.org/10.1016/J.POSTHARVBIO.2014.02.004
- Espinosa-Leal CA, Mora-Vásquez S, Puente-Garza CA & Alvarez‑Sosa DS (2022). Recent advances on the use of abiotic stress (water, UV radiation, atmospheric gases, and temperature stress) for the enhanced production of secondary metabolites on in vitro plant tissue culture. Plant Growth Regulation 97: 1–20. https://doi.org/10.1007/s10725-022-00810-3
- Formica-Oliveira AC, Martínez-Hernández GB, Díaz-López V, Artés F & Artés-Hernández F (2017). Use of postharvest UV-B and UV-C radiation treatments to revalorize broccoli byproducts and edible florets. Innovative Food Science & Emerging Technologies 43: 77–83. https://doi.org/10.1016/J.IFSET.2017.07.036
- Guerrero-Beltr·n JA & Barbosa-C·novas GV (2004). Advantages and limitations on processing foods by UV light. Food Science and Technology International 10(3): 137–147. https://doi:10.1177/1082013204044359
- Hagen SF, Borge GIA, Bengtsson GB, Bilger W, Berge A, Haffner K & Solhaug KA (2007). Phenolic contents and other health and sensory related properties of apple fruit (Malus domestica Borkh., cv. Aroma), Effect of postharvest UV-B irradiation. Postharvest Biology and Technology 45(1): 1–10. https://doi.org/10.1016/J.POSTHARVBIO.2007.02.002
- Interdonato R, Rosa M, Nieva CB, González JA, Hilal M & Prado FE (2011). Effects of low UV-B doses on the accumulation of UV-B absorbing compounds and total phenolics and carbohydrate metabolism in the peel of harvested lemons. Environmental and Experimental Botany 70(2–3): 204–211. https://doi.org/10.1016/J.ENVEXPBOT.2010.09.006
- Janghu S, Kumar V & Yadav AK (2024). Post-harvest management of fruits and vegetables. Current Perspectives in Agriculture and Food Science 7: 125–148. https://doi.org/10.9734/bpi/cpafs/v7/7984E
Jansen MAK, Hectors K, O’Brien NM, Guisez Y & Potters G (2008). Plant stress and human health: Do human consumers benefit from UV-B acclimated crops? Plant Science 175(4): 449–458. https://doi.org/10.1016/J.PLANTSCI.2008.04.010
- Kang HM & Saltveit ME (2002). Antioxidant capacity of lettuce leaf tissue increases after wounding. Journal of Agricultural and Food Chemistry 50(26): 7536–7541. https://doi.org/10.1021/JF020721C
- Kasim MU & Kasim R (2017a). The effects of ultraviolet B (UV-B) irradiation on color quality and decay rate of Capia pepper during postharvest storage. Food Science and Technology International 38(2): 363–368. https://doi.org/10.1590/1678-457X.05817
- Kasim MU & Kasim R (2017b). Yellowing of fresh-cut spinach (Spinacia oleracea L.) leaves delayed by UV-B applications. Information Processing in Agriculture 4(3): 214–219. https://doi.org/10.1016/J.INPA.2017.05.006
- Kim MJ, Moon Y, Tou JC, Mou B & Waterland NL (2016). Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.). Journal of Food Composition and Analysis 49: 19–34. https://doi.org/10.1016/J.JFCA.2016.03.004
- Koutchma T (2009). Advances in ultraviolet light technology for non-thermal processing of liquid foods. Food and Bioprocess Technology 2(2): 138–155. https://doi.org/10.1007/S11947-008-0178-3/FIGURES/3
Koutchma T (2019). Ultraviolet light in food technology: Principles and applications. New york: CRC Press. 337 p.
- Liu C, Han X, Cai L, Lu X, Ying T & Jia Z (2011). Postharvest UV-B irradiation maintains sensory qualities and enhances antioxidant capacity in tomato fruit during storage. Postharvest Biology and Technology 59(3): 232–237. https://doi.org/10.1016/J.POSTHARVBIO.2010.09.003
- Liu X, Ardo S, Bunning M, Parry J, Zhou K, Stushnoff C, Stoniker F, Yu L & Kendall P (2007). Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT-Food Science and Technology 40(3): 552–557. https://doi.org/10.1016/J.LWT.2005.09.007
- Llorach R, Tomás-Barberán FA & Ferreres F (2004). Lettuce and chicory by products as a source of antioxidant phenolic extracts. Journal of Agricultural and Food Chemistry 52(16): 5109–5116. https://doi.org/10.1021/JF040055A
- Mampholo BM, Maboko MM, Soundy P & Sivakumar D (2016). Phytochemicals and overall quality of leafy lettuce (Lactuca sativa L.) varieties grown in closed hydroponic system. Journal of Food Quality 39(6): 805–815. https://doi.org/10.1111/JFQ.12234
- Martínez-Ispizua E, Calatayud Á, Marsal JI, Basile F, Cannata C, Abdelkhalik A, Soler S, Valcárcel JV & Martínez-Cuenca MR (2022). Postharvest changes in the nutritional properties of commercial and traditional lettuce varieties in relation with overall visual quality. Agronomy 12(2): 403. https://doi.org/10.3390/AGRONOMY12020403/S1
- Muscolo A, Marra F, Canino F, Maffia A, Mallamaci C & Russo M (2022). Growth, nutritional quality and antioxidant capacity of lettuce grown on two different soils with sulphur-based fertilizer, organic and chemical fertilizers. Scientia Horticulturae 305: 111421. https://doi.org/10.1016/J.SCIENTA.2022.111421
- Nadkarni BY (1965). Determination of ascorbic acid in coloured extracts: A new modification of the indophenol technique. Mikrochimica Acta 53(1): 21–27. https://doi.org/10.1007/BF01218730/METRICS
- Nguyen CTT, Kim J, Yoo KS, Lim S & Lee EJ (2014). Effect of prestorage UV-A, -B, and -C radiation on fruit quality and anthocyanin of “Duke” blueberries during cold storage. Journal of Agricultural and Food Chemistry 62(50): 12144–12151. https://doi.org/10.1021/JF504366X/ASSET/IMAGES/MEDIUM/JF-2014-04366X_0005.GIF
- Nicolle C, Carnat A, Fraisse D, Lamaison JL, Rock E, Michel H, Amoureux P & Remesy C (2004). Characterisation and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). Journal of the Science of Food and Agriculture 84(15): 2061–2069. https://doi.org/10.1002/JSFA.1916
- Pathare PB, Opara UL & Al-Said FAj (2013). Colour measurement and analysis in fresh and processed foods: A review. Food Bioprocess Technology 6: 36–60. https://doi.org/10.1007/s11947-012-0867-9
- Paul ND, Moore JP, McPherson M, Lambourne C, Croft P, Heaton JC & Wargent JJ (2012). Ecological responses to UV radiation: Interactions between the biological effects of UV on plants and on associated organisms. Physiologia Plantarum 145(4): 565–581. https://doi:10.1111/j.1399-3054.2011.01553.x
- Pérez-López U, Miranda-Apodaca J, Muñoz-Rueda A & Mena-Petite A (2013). Lettuce production and antioxidant capacity are differentially modified by salt stress and light intensity under ambient and elevated CO2. Journal of Plant Physiology 170(17): 1517–1525. https://doi.org/10.1016/J.JPLPH.2013.06.004
- Salemi S, Saedisomeolia A, Azimi F, Zolfigol S, Mohajerani E, Mohammadi M & Yaseri M (2021). Optimizing the production of vitamin D in white button mushrooms (Agaricus bisporus) using ultraviolet radiation and measurement of its stability. Lebensmittel-Wissenschaft + [i.e. Und] Technologie. Food Science + Technology. Science + Technologie Alimentaire 137: 110401. https://doi.org/10.1016/J.LWT.2020.110401
- Scattino C, Negrini N, Morgutti S, Cocucci M, Crisosto CH, Tonutti P, Castagna A & Ranieri A (2016). Cell wall metabolism of peaches and nectarines treated with UV-B radiation: A biochemical and molecular approach. Journal of the Science of Food and Agriculture 96(3): 939–947. https://doi.org/10.1002/JSFA.7168
- Tikekar RV, Anantheswaran RC, Elias RJ & Laborde LF (2011). Ultraviolet-induced oxidation of ascorbic acid in a model juice system: Identification of degradation products. Journal of Agricultural and Food Chemistry 59(15): 8244–8248. https://doi.org/10.1021/JF201000X
- Tomás-Callejas A, Otón M, Artés F & Artés-Hernández F (2012). Combined effect of UV-C pretreatment and high oxygen packaging for keeping the quality of fresh-cut Tatsoi baby leaves. Innovative Food Science & Emerging Technologies 14: 115–121. https://doi.org/10.1016/J.IFSET.2011.11.007
- Usall J, Ippolito A, Sisquella M & Neri F (2016). Physical treatments to control postharvest diseases of fresh fruits and vegetables. Postharvest Biology and Technology 122: 30–40. https://doi.org/10.1016/J.POSTHARVBIO.2016.05.002
- Wang W, Zhang C, Shang M, Lv H, Liang B, Li J & Zhou W (2022). Hydrogen peroxide regulates the biosynthesis of phenolic compounds and antioxidant quality enhancement in lettuce under low nitrogen condition. Food Chemistry: X 16: 100481. https://doi.org/10.1016/J.FOCHX.2022.100481