BibTex RIS Kaynak Göster

Ratlarda Laminektomi Sonrası Aşırı Epidural Fibrozis Gelişmesinde Nitrik Oksitin Etkileri: Bir Ön Çalışma

Yıl 2016, Cilt: 5 Sayı: 1, 1 - 5, 01.04.2016

Öz

Amaç: Laminektomi ve/veya diskektomi sonrası, tüm önleyici işlemlere rağmen, hastaların bir alt grubunda aşırı epidural fibrozis gelişmektedir. Bizim hipotezimiz, laminektomi sonrası aşırı fibrosis gelişen hastalarda yara iyileşmesinin geç safhasında nitrik oksit NO üretiminin arjinaz tarafından inhibisyonunda bir defektin olduğu şeklindedir. Amacımız, bir hayvan laminektomi modelinde aşırı fibrozis gelişmesi üzerinde NO ve arjinaz’ın etkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmada, 50 erkek wistar rat beş gruba ayrıldı; tümüne lomber laminektomi yapıldı. Laminektomi sonrası 3. ve 12. gün arasında, 1. gruba kontrol serum fizyolojik, 2. gruba; indüklenebilir nitrik oksit sentazı iNOS uyarmak üzere lipopolisakkarit LPS , 3. gruba LPS ve L-arginin NOS substratı , 4. gruba gliseril trinitrat NO donörü ve 5. gruba L-valine arjinaz inhibitörü intraperitonal olarak verildi. Sonuçlar: Hayvanların tümü 12. gün sakrifiye edildi ve hayvanların tümünde epidural fibrozis gross ve histolojik inceleme ile değerlendirildi. 2. grupta yara iyileşmesi ve fibrozis zayıf iken, 3'üncü grupta hafif derecede kompresyon yapan bir epidural fibrozis vardı. 4. ve 5'inci gruplarda ise ciddi derecede kompresyon yapan bir epidural fibrozis vardı. Tartışma: iNOS inhibe edilerek NO üretiminin azalması ve L-arjinin’in büyük oranda arjinaz tarafından kullanılması, yara iyileşmesinin geç dönemindeki tamir ve yenilenme işlemini etkileyen en önemli faktörler olduğu kanaatindeyiz

Kaynakça

  • Brotchi J, Pirotte B, De Witte O, Levivier M. Prevention of epidural fibrosis in a prospective series of 100 primary lumbo-sacral discectomy patients: follow-up and assessment at re- operation. Neurol Res 1999; 21 Suppl 1: 47-50.
  • Cekinmez M, Sen O, Atalay B, Erdogan B, Baybek M, Caner H, et al: Effects of methyl prednisolone acetate, fibrin glue and combination of methyl prednisolone acetate and fibrin glue in prevention of epidural fibrosis in a rat model. Neurol Res 2010; 32: 700-5.
  • Martin-Ferrer S. Failure of autologous fat grafts to prevent postoperative epidural fibrosis in surgery of the lumbar spine. Neurosurgery 1989; 24(5): 718-21.
  • Schaffer MR, Tantry U, Gross SS, Wasserkrug HL, Barbul A: Nitric oxide regulates wound healing. J Surg Res 1996, 63: 237-40
  • Alexander JW, Supp DM. Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection. Adv Wound Care (New Rochelle) 2014; 3(11): 682-90.
  • Stechmiller JK, Childress B, Cowan L. Arginine supplementation and wound healing. Nutr Clin Pract 2005; 20(1): 52-61.
  • Lee RH, Efron D, Tantry U, Barbul A. Nitric oxide in the healing wound: A time-course study. J Surg Res 2001; 101: 104–8.
  • Tao H, Wang Q, LU R. Prevention of epidural scarr adhesion following lumb spine operation. Chung Hua Wai Ko Tsa Chih 1996; 34(6): 344-7.
  • Burton CV, Kirkaldy-Willis WH, Yong-Hing K, et al. Causes of failure of surgery on the lumbar spine. Clin Orthop 1981; 157: 191-9.
  • Finnegan WJ, Fenlin JM, Marvel JP, et al. Results of surgical intervention in the symtomatic multiply-operated back patient: analysis of sixty- seven cases followed for three to seven years. J Bone Joint Surg (Am) 1979; 61-A: 1077-82.
  • Gabriel EM, Friedman AH. The failed back surgery syndrome in Neurosurgery. In: Wilkins RH, Rengachary SS, eds. New York, St. Louis, San Professions Division 1996; 2nd edition, Vol. IIIB: 3863-70. Co, Health
  • Kim SS, Michelsen CB. Revision surgery for failed back surgery syndrome. Spine 1992; 17: 957-60.
  • Nussbaum CE, McDonald JV, Baggs RB. Use of Vicryl (polyglactin 910) mesh to limit epidural scar formation after laminectomy. Neurosurgery 1990; 26: 649-54.
  • Robertson JT, Maier K, Anderson RW, Mule JL, Palatinsky EA. Prevention of epidural fibrosis with ADCON-L in presence of a duratomy during lumbar disc surgery: experiences with a preclinical model. Neuro Res 1999; 21 (Suppl 1): 561-6.
  • Vural E, Yilmaz M, Ilbay K, Ilbay G. Prevention of Administration of Mitomycin C or Daunorubicin. Turk Neurosurg 2016; 26(2): 291-6. by Local
  • Thorton FJ, Schaffer MR, Witte MB, et al. Enhanced collagen accumulation following direct transfection of the inducible nitric oxide synthase gene in cutaneous wounds. Biochem Biophys Res Commun 1998; 246 (3): 654-9.
  • Efron D, Kirk SJ, Regan MC, Wasserkrug HL, Barbul A. Nitric oxide generation from L-arginine is lymphocyte DNA synthesis. Surgery 1991; 110: 27-334. peripheral blood
  • McCall TB, Boughton-Smith NK, Palmer RM, Whittle BJ, Moncada S. Synthesis of nitric oxide from L-arginine by neutrophils. Release and interaction with superoxide anion. Biochem J 1989; 261: 293-6.
  • Schaffer MR, Tantry U, Van Wesep RA, Barbul A. Nitric oxide metabolism in wounds. J Surg Res 1997; 71(1): 25-31.
  • Schaffer MR, Efron PA, Thorton FJ, et al. Nitric oxide, an autocrine regulator of wound fibroblast synthetic function. J Immunol 1997; 158(5): 2375- 81.
  • Schaffer MR, Tantry U, Thornton FJ, Barbul A. Inhibition of nitric oxide synthesis in wounds: pharmacology and effect on accumulation of collagen in wounds in mice. Eur J Surg 1999; 165(3): 262-7. 22. Stuehr DJ, Marletta MA. Induction of nitrite/nitrate synthesis in murine macrophages by BCG infection, lymphokines, or interferon- [gamma]. J Immunol 1987; 140: 518-25.
  • Wang R, Ghahary A, Shen YJ, Scott PG, Tredget EE. Human dermal fibroblasts produce nitric oxide and express both constituve and inducible nitric oxide synthase isoforms. J Invest Dermatol 1996, 106(3): 419-27.
  • Werner-Felmayer G, Werner ER, Fuchs D, Hausen Tetrahydrobiopterin-dependent nitrite and nitrate in murine fibroblasts. J Exp Med 1990; 172: 1599-607. G, Wachter formation of
  • Witte MB, Schäffer MR, Barbul A. Phenotypic induction of nitric oxide is critical for synthetic function in wound fibroblasts. Surg Forum 1996; 47: 703-5
  • Krieg T, Braun-Falco O. Fibrocytes and fibroblasts: Definition and significance in wound healing and fibrotic disease of the skin. Z Hautkr 1989; 64 (9): 775-81.
  • Durante W, Johnson FK, Johnson RA. Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol 2007; 34(9): 906-11.
  • Klasen S, Hammermann R, Fuhrmann M, Lindemann D, Beck KF, Pfeilschifter J, Racke K. Glucocorticoids induced up-regulation of arginase in rat alveolar macrophages. Br J Pharmacol 2001; 132(6): 1349- 57.
  • Imagama T, Ogino K, Takemoto K, Kato Y, Kataoka H, Suzuki H, Ran Z, Setiawan H, Fujikura Y, Taguchi T. Regulation of nitric oxide generation by up-regulated arginase I in rat spinal cord injury. J Clin Biochem Nutr 2012; 51(1): 68- 75.

The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study

Yıl 2016, Cilt: 5 Sayı: 1, 1 - 5, 01.04.2016

Öz

Aim: In spite of all preventive procedures, extensive epidural fibrosis following laminectomy or/and dissectomy still remains a major problem. We hypothesized that the patients, who suffered from extensive fibrosis, have a defect in inhibition of nitric oxide NO production that make increase in fibroblastic activity and collagen accumulation in healing wound. Aim of this study is to explore the effect of NO and arginase on extensive fibrosis in an animal model of laminectomy. Material and Method: 50 male wistar rats are divided in five groups; all of them were made laminectomy in lumbar spine. Between the 3rd and 12th days, we gave saline to the group1 control group , lipopolysaccharide LPS to the group 2 to induce inducible nitric oxide synthase iNOS , LPS plus L-arginine NOS substrate to the group 3, glyceryl trinitrate NO donor to the group 4 and L-valine an arginase inhibitor to the group 5 intraperitonally. Results: All of these animals were sacrificed on the 12th day after operation, and the epidural fibrosis of everyanimal was evaluated by gross observation and histological examinations. While in the group 2 wound healing and fibrosis was poor, in the group 3, there was mild degree epidural fibrosis, but epidural fibrosis was not compressive. In group 4 and 5 there were severe degree compressive extensive epidural fibrosis. Conclusion: It was concluded that the reduction of NO production by inhibiting iNOS and largely use of Larginine by arginase are the most important factors that affect the process of repair and remodeling in the late period of wound healing, therefore, the absence or insufficiency in the NOS inhibition or extremism in NO production during late period of the wound healing leads to extensive epidural fibrosis

Kaynakça

  • Brotchi J, Pirotte B, De Witte O, Levivier M. Prevention of epidural fibrosis in a prospective series of 100 primary lumbo-sacral discectomy patients: follow-up and assessment at re- operation. Neurol Res 1999; 21 Suppl 1: 47-50.
  • Cekinmez M, Sen O, Atalay B, Erdogan B, Baybek M, Caner H, et al: Effects of methyl prednisolone acetate, fibrin glue and combination of methyl prednisolone acetate and fibrin glue in prevention of epidural fibrosis in a rat model. Neurol Res 2010; 32: 700-5.
  • Martin-Ferrer S. Failure of autologous fat grafts to prevent postoperative epidural fibrosis in surgery of the lumbar spine. Neurosurgery 1989; 24(5): 718-21.
  • Schaffer MR, Tantry U, Gross SS, Wasserkrug HL, Barbul A: Nitric oxide regulates wound healing. J Surg Res 1996, 63: 237-40
  • Alexander JW, Supp DM. Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection. Adv Wound Care (New Rochelle) 2014; 3(11): 682-90.
  • Stechmiller JK, Childress B, Cowan L. Arginine supplementation and wound healing. Nutr Clin Pract 2005; 20(1): 52-61.
  • Lee RH, Efron D, Tantry U, Barbul A. Nitric oxide in the healing wound: A time-course study. J Surg Res 2001; 101: 104–8.
  • Tao H, Wang Q, LU R. Prevention of epidural scarr adhesion following lumb spine operation. Chung Hua Wai Ko Tsa Chih 1996; 34(6): 344-7.
  • Burton CV, Kirkaldy-Willis WH, Yong-Hing K, et al. Causes of failure of surgery on the lumbar spine. Clin Orthop 1981; 157: 191-9.
  • Finnegan WJ, Fenlin JM, Marvel JP, et al. Results of surgical intervention in the symtomatic multiply-operated back patient: analysis of sixty- seven cases followed for three to seven years. J Bone Joint Surg (Am) 1979; 61-A: 1077-82.
  • Gabriel EM, Friedman AH. The failed back surgery syndrome in Neurosurgery. In: Wilkins RH, Rengachary SS, eds. New York, St. Louis, San Professions Division 1996; 2nd edition, Vol. IIIB: 3863-70. Co, Health
  • Kim SS, Michelsen CB. Revision surgery for failed back surgery syndrome. Spine 1992; 17: 957-60.
  • Nussbaum CE, McDonald JV, Baggs RB. Use of Vicryl (polyglactin 910) mesh to limit epidural scar formation after laminectomy. Neurosurgery 1990; 26: 649-54.
  • Robertson JT, Maier K, Anderson RW, Mule JL, Palatinsky EA. Prevention of epidural fibrosis with ADCON-L in presence of a duratomy during lumbar disc surgery: experiences with a preclinical model. Neuro Res 1999; 21 (Suppl 1): 561-6.
  • Vural E, Yilmaz M, Ilbay K, Ilbay G. Prevention of Administration of Mitomycin C or Daunorubicin. Turk Neurosurg 2016; 26(2): 291-6. by Local
  • Thorton FJ, Schaffer MR, Witte MB, et al. Enhanced collagen accumulation following direct transfection of the inducible nitric oxide synthase gene in cutaneous wounds. Biochem Biophys Res Commun 1998; 246 (3): 654-9.
  • Efron D, Kirk SJ, Regan MC, Wasserkrug HL, Barbul A. Nitric oxide generation from L-arginine is lymphocyte DNA synthesis. Surgery 1991; 110: 27-334. peripheral blood
  • McCall TB, Boughton-Smith NK, Palmer RM, Whittle BJ, Moncada S. Synthesis of nitric oxide from L-arginine by neutrophils. Release and interaction with superoxide anion. Biochem J 1989; 261: 293-6.
  • Schaffer MR, Tantry U, Van Wesep RA, Barbul A. Nitric oxide metabolism in wounds. J Surg Res 1997; 71(1): 25-31.
  • Schaffer MR, Efron PA, Thorton FJ, et al. Nitric oxide, an autocrine regulator of wound fibroblast synthetic function. J Immunol 1997; 158(5): 2375- 81.
  • Schaffer MR, Tantry U, Thornton FJ, Barbul A. Inhibition of nitric oxide synthesis in wounds: pharmacology and effect on accumulation of collagen in wounds in mice. Eur J Surg 1999; 165(3): 262-7. 22. Stuehr DJ, Marletta MA. Induction of nitrite/nitrate synthesis in murine macrophages by BCG infection, lymphokines, or interferon- [gamma]. J Immunol 1987; 140: 518-25.
  • Wang R, Ghahary A, Shen YJ, Scott PG, Tredget EE. Human dermal fibroblasts produce nitric oxide and express both constituve and inducible nitric oxide synthase isoforms. J Invest Dermatol 1996, 106(3): 419-27.
  • Werner-Felmayer G, Werner ER, Fuchs D, Hausen Tetrahydrobiopterin-dependent nitrite and nitrate in murine fibroblasts. J Exp Med 1990; 172: 1599-607. G, Wachter formation of
  • Witte MB, Schäffer MR, Barbul A. Phenotypic induction of nitric oxide is critical for synthetic function in wound fibroblasts. Surg Forum 1996; 47: 703-5
  • Krieg T, Braun-Falco O. Fibrocytes and fibroblasts: Definition and significance in wound healing and fibrotic disease of the skin. Z Hautkr 1989; 64 (9): 775-81.
  • Durante W, Johnson FK, Johnson RA. Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol 2007; 34(9): 906-11.
  • Klasen S, Hammermann R, Fuhrmann M, Lindemann D, Beck KF, Pfeilschifter J, Racke K. Glucocorticoids induced up-regulation of arginase in rat alveolar macrophages. Br J Pharmacol 2001; 132(6): 1349- 57.
  • Imagama T, Ogino K, Takemoto K, Kato Y, Kataoka H, Suzuki H, Ran Z, Setiawan H, Fujikura Y, Taguchi T. Regulation of nitric oxide generation by up-regulated arginase I in rat spinal cord injury. J Clin Biochem Nutr 2012; 51(1): 68- 75.
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

M Arif Aladag Bu kişi benim

Yusuf Turkoz Bu kişi benim

Engin Sahna Bu kişi benim

Nureddin Cengiz Bu kişi benim

Hakan Parlakpınar Bu kişi benim

O Faruk Cihan Bu kişi benim

Mehmet Gul Bu kişi benim

Yayımlanma Tarihi 1 Nisan 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 5 Sayı: 1

Kaynak Göster

APA Aladag, M. A., Turkoz, Y., Sahna, E., Cengiz, N., vd. (2016). The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study. Annals of Health Sciences Research, 5(1), 1-5.
AMA Aladag MA, Turkoz Y, Sahna E, Cengiz N, Parlakpınar H, Cihan OF, Gul M. The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study. Ann Health Sci Res. Nisan 2016;5(1):1-5.
Chicago Aladag, M Arif, Yusuf Turkoz, Engin Sahna, Nureddin Cengiz, Hakan Parlakpınar, O Faruk Cihan, ve Mehmet Gul. “The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study”. Annals of Health Sciences Research 5, sy. 1 (Nisan 2016): 1-5.
EndNote Aladag MA, Turkoz Y, Sahna E, Cengiz N, Parlakpınar H, Cihan OF, Gul M (01 Nisan 2016) The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study. Annals of Health Sciences Research 5 1 1–5.
IEEE M. A. Aladag, Y. Turkoz, E. Sahna, N. Cengiz, H. Parlakpınar, O. F. Cihan, ve M. Gul, “The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study”, Ann Health Sci Res, c. 5, sy. 1, ss. 1–5, 2016.
ISNAD Aladag, M Arif vd. “The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study”. Annals of Health Sciences Research 5/1 (Nisan 2016), 1-5.
JAMA Aladag MA, Turkoz Y, Sahna E, Cengiz N, Parlakpınar H, Cihan OF, Gul M. The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study. Ann Health Sci Res. 2016;5:1–5.
MLA Aladag, M Arif vd. “The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study”. Annals of Health Sciences Research, c. 5, sy. 1, 2016, ss. 1-5.
Vancouver Aladag MA, Turkoz Y, Sahna E, Cengiz N, Parlakpınar H, Cihan OF, Gul M. The Effects of Nitric Oxide on Extensive Epidural Fibrosis After Laminectomy in Rats: A Preliminary Study. Ann Health Sci Res. 2016;5(1):1-5.