Immunohistochemical Evaluation of IFN-γ levels in sheep with cystic echinococcosis
Öz
In the present study, with an aim to demonstrate in detail the cellular immune response given to the parasite in the lungs of sheep infected with cystic echinococcosis (CE), a comparative assessment was made of the interferon-gamma (IFN-γ) levels of healthy and infected animals using immunohistochemical methods. The material of this study consisted of lung tissue samples taken from an average of 10 dead sheep brought to Faculty of Veterinary Medicine Department of Pathology for routine diagnosis. Lung tissue samples taken from 10 healthy sheep without any pathological lesions were also used for control purposes. Hematoxylin & Eosin staining was applied to the sections in order to evaluate the histopathological changes in the tissues. Stained with IFN-γ commercial antibody using the Avidin-Biotin Peroxidase-Technique in accordance with the manufacturer's procedures. Macroscopic examination revealed large and small oval-shaped cystic structures that spread to almost all lobes of the lung. In the histopathological examination of the lungs, it was observed that the cysts were surrounded by a fibrous capsule and there was a concentric lamellated cyst wall just below the fibrous capsule. It was determined that IFN-γ levels increased statistically in the CE group compared to the control group. Such an increase in IFN-γ levels reveals that this marker is a remarkable cytokine in the diagnosis of parasitic diseases such as CE. The fact that IFN-γ staining is much more severe in phagocytes such as giant cells and macrophages, which play a dominant role in the cellular immune response developed against parasitic infection, shows that this pro-inflammatory cytokine may be effective in anti-echinococcal therapy.
Anahtar Kelimeler
Cellular Defense, Cystic Echinococcosis, Cytokine, Interferon-γ, Sheep
Kaynakça
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