Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein / Bovine Respiratory Syncytial Virus G Glycoproteininin Filogenetik ve Analizi ve Genetik Karakterizasyonu
Öz
This study aimed to molecularly detect Bovine Respiratory Syncytial Virus (BRSV) in cattle with clinical signs of respiratory system infection and to conduct a phylogenetic analysis of the detected strains. To detect the pathogen, nasal swab samples collected from 96 cattle were subjected to Reverse Transcriptase – nested PCR using specific primer sets. As a result of the molecular techniques, viral nucleic acid was detected in 2.08% (2/96) of the samples. Additionally, sequence and phylogenetic analyses of the detected local variant were performed and compared with various BRSV strains identified in different regions of Turkey and around the world. The genetic data obtained revealed that the isolates identified in this study belong to Subgroup III, alongside BRSV strains reported from various regions of Turkey, France, and the USA. Immunologically significant point mutations were detected in the immunodominant G glycoprotein region of the variant (Asn(N)179 → Asp(D) / Ala(A) 205 → Thr (T)). This result indicates that BRSV strains circulating among cattle can escape the host immune system without losing their pathogenicity and is considered to be one of the important factors that can cause respiratory system infection outbreaks in cattle.
Anahtar Kelimeler
“Cattle”, “BRSV”, “RT-nPCR”, “Genetic Characterization”
Destekleyen Kurum
The authors did not receive support from any organization for the submitted work.
Etik Beyan
Approval was obtained from the animal testing local ethics committee of Burdur Mehmet Akif Ersoy University. The procedures used in this study adhere to the tenets of the Declaration of Helsinki (MAKU-HADYEK 1045/2023)
Kaynakça
- Bertolotti, L., Giammarioli, M., & Rosati, S. (2018). Genetic characterization of bovine respiratory syncytial virus strains isolated in Italy: evidence fort he circulation of new divergent clades. Journal of Veterinary Diagnostic Investigation, 30, 300-304. https://doi.org/ 10.1177/1040638717746202.
- Bidokhti, M.R.M., Traven, M., Ohlson, A., Zarnegar, B., Baule, C., Belak, S., Alenius, S., & Liu, L. (2012). Phylogenetic analysis of bovine respiratory syncytial viruses from recent outbreaks in feedlot and dairy cattle herds. Archive of Virology, 157, 601-607. https://doi.org/ 10.1007/s00705-011-1209-3
- Chang, Y., Yue, H., & Tang, C. (2022). Prevalence and molecular characteristics of bovine respiratory syncytial virus in beef cattle in China. Animals, 12, 3511. https://doi.org/10.3390/ani12243511
- Ellis, J. (2013). Bovine respiratory syncytial virus. In: Munir M. (ed), Mononegaviruses of Veterinary Importance: Pathobiology and Molecular Diagnosis (pp.117-140). CPI Group.
- İnce, Ö.B., Şevik, M., Özgür, E.G., & Sait A. (2022). Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey. Tropical Animal Health and Production, 54, 4. https://doi.org/10.1007/s11250-021-03022-5.
- Jia, S., Yao, X., Yang, Y., Niu, C., Zhao, Y., Zhang, X., Pan, R., Jiang, X., Xiaobo, S., Qiao, X., Guan, X., & Xu, Y. (2021). Isolation, identification, and phylogenetic analysis of subgroup III strain of bovine respiratory syncytial virus contributed to outbreak of acute respiratory disease among cattle in Northeast China. Virulence, 12, 404-414. https://doi.org/10.1080/21505594.2021.1872178
- Karayel-Hacıoğlu, I., Coşkun, N., Duran-Yelken, S., Sevinç, S., & Alkan, F. (2019). Phylogenetic analysis of bovine respiratory syncytial virus from calves with respiratory disorders. Kafkas Universitesi Veteriner Fakultesi Dergisi. 25, 251-256. https://doi.org/ 10.9775/kvfd.2018.20819
- Klem, T.B., Rimstad, E., & Stokstad, M. (2014). Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway. BMC Veterinary Research, 10, 15. https://doi.org/10.1186/1746-6148-10-15
- Kresic, N., Bedekovic, T., Brnic, D., Simic, I., Lojkic, I., & Turk, N. (2018). Genetic analysis of bovine respiratory syncytial virus in Crotia. Comparative Immunology. Microbiology and Infectious Diseases, 58, 52-57. https://doi.org/10.1016/j.cimid.2018.09.004.
- Kumagai, A., Kawauchi, K., Andoh, K., & Hatama, S. (2021). Sequence and unique phylogeny of G genes of bovine respiratory syncytial viruses circulating in Japan, Journal of Veterinary Diagnosis Investigation 33, 162-166. https://doi.org/10.1177/1040638720975364.