Soya Bitkisindeki Glutatyon Redüktaz Aktivitesi ve mRNA Seviyesinin Kuraklık Stresinde Salisilik Asit ile Değişimleri

Abstract

Bu çalışma, kuraklık stresi ve salisilik asitin (SA) soya bitkisindeki etkilerini değerlendirmek için gerçekleştirilmiştir. Bu amaçla, su stresine maruz bırakılan soya bitkisindeki SA, reaktif oksijen türleri (ROS) ve glutatyon redüktaz (GR) enzimi arasındaki ilişki incelenmiştir. Soya fasulyesi (Glycine max L. cv.) bitkileri serada kum tepsilere ekilerek büyütülmüştür. İkinci yaprak tamamen çıktığında bitkilerin yarısı bir hafta süreyle kuraklığa maruz bırakılmıştır. Bir haftanın sonunda hem kontrol hemde kuraklık stres grupları SA (200 μmol/L) ile muamele edilmiş ve iki gün sonra tüm gruplardan kesimler alınmıştır. Kontrol, kontrol+SA, kuraklık, kuraklık+SA muameleli yapraklarda glutatyon redüktaz aktivitesi ve mRNA seviyeleri ölçülmüştür. Bu çalışmada, 200 μmol/L SA muamelesi kontrol şartlarında GR aktivitesini önemli ölçüde artırmıştır. Kuraklık muameleli yapraklarda, kontroller ile karşılaştırıldığında, GR enzim aktivitesinin arttığı gözlenmiştir. Ancak su stresi altında SA muamelesi ile GR enzim aktivitesi önemli ölçüde azalmıştır. GR enzimini kodlayan genlerin transkripsiyon seviyeleri real-time PCR (Polimeraz zincirleme tepkimesi) kullanılarak ölçülmüştür. SA muamelesi kuraklığa maruz kalan soya yapraklarının GR-RNA seviyelerini hızlı bir şekilde azaltmıştır.

Keywords

• Glutatyon redüktaz,kuraklık,salisilik asit,soya

Glutathione Reductase Activity in Soybean Plants and Changes in mRNA Levels with Salicylic Acid in Drought Stress

Abstract

This study was carried to evaluate the effect of drought stress and salicylic acid (SA) treatments in soybean plants. Soybean (Glycinemax L. cv.) plants were grown to sown in trays of sand in greenhouse. When the second leaf was fully expanded, half of the plants were exposed to drought stress for one week. At the end of one week, half of the plants in the both control and drought stress groups were treated with SA (200 μmol/L) and two days later was taken cuttings from the whole. The activities of glutathione reductase (GR) and levels of mRNA have been measured in control, control-SA treatment, drought treament and drought-SA treatment leaves. In this study, 200 μmol/L SA treatment significantly has been increased GR activity in control conditions. Drought treated leaves have been observed elevated in the activities of the GR enzyme, compared to controls. But, under medium water deficit, GR activity significantly reducing with SA treatment. The transcript levels of the genes encoding GR enzyme have been measured using quantitative real-time PCR (Polymerase Chain Reaction). SA treatment has decreased rapidly GR -RNA levels of soybean leaves exposed to drought stress.

Keywords

• Glutathione reductase,drought,salicylic acid,soybean

References

1. [1] Ş. Kuşvuran, “Kavunlarda Kuraklık ve Tuzluluğa Toleransın Fzyolojik Mekanizmaları Arasındaki Bağlantılar,” Doktora Tezi, Çukurova Üniversitesi: Fen Bilimleri Enstitüsü, Adana, 2010.
2. [2] A.R. Reddy, K.V. Chaitanya, P.P. Jutur, K. Sumithra, “Differential Antioxidative Responses to Water Stress Among Five Mulberry (Morus alba L.) Cultivars”, Environ Exp.Bot, vol.52, pp. 33– 42, 2004.
3. [3] M.R. Simaei, A. Khavari-Nejad, F. Bernard, “Exogenous Application of Salicylic Acid and Nitric Oxide on the Ionic Contents and Enzymatic Activities in NaCl-Stressed Soybean Plants”, Am J Plant Sci, vol.3, pp.1495-1503, 2012.
4. [4] A. Bano, F. Ullah, A. Nosheen, “Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat” Plant Soil Environ. vol.58, no.4, pp.181–185, 2012.
5. [5] E Tasgın, Ö Atıcı, B Nalbantoğlu, L.P. Popova, “Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves”, Phytochem, vol.67, pp.710-715, 2006.
6. [6] D. Contour-Ansel, L.M. Torres-Franklın, M.H. Cruz de Carvalho, A. D’arcy-Lameta, Y. Zuily-Fodil, “Glutathione Reductase in Leaves of Cowpea: Cloning of Two cDNAs, Expression and Enzymatic Activity under Progressive Drought Stress. Desiccation and Abscisic Acid Treatment”, Ann of Bot, vol.98, pp.1279–1287, 2006.
7. [7] G. Pastori, C.H. Foyer, “Mullineaux P. Low temperature-induced changes in the distribution of H2O2 and antioxidants between the bundle sheath and mesophyll cells of maize leaves”, J Exp Bot, vol 51, pp. 107–113, 2000.
8. [8] A.C.F. Vasconcelos, X. Zhang, E.H. Ervin, J.C. Kiehl, Enzymatıc Antioxidant Responses to Biostimulants ın Maize and Soybean Subjected To Drought, Sci. Agric. (Piracicaba, Braz.) vol. 66, no.3, pp. 395-402,2009.

9. [9] H. Kaminaka, S. Morita, M. Nakajima, T. Masumura, K. Tanaka. “Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.)”, Plant Cell Physiol, vol. 39, no.12, pp.1269-1280,1998.
10. [10] M. Jiang, J. Zhang, “Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves,” J Exp Bot, vol.53, no.379, pp. 2401-2410, 2002.
11. [11] M.H. Cruz de Carvalho, D. Contour-Ansel, “(h)GR, beans and drought stress”, Plant Sig& Beh vol.3, no.10, pp. 834-835,2008.
12. [12] G. Kang, G. Li, W. Xu, X. Peng, Q. Han, Y. Zhu, T. Guo, “Proteomics reveals the effects of salicylic acid on growth and tolerance to subsequent drought stress in wheat” J Prot Res, vol.11, pp. 6066–6079, 2012.
13. [13] M. Zarghami, M. Shoor, A. Ganjali, N. Moshtaghi, A. Tehranifar, “Effect of salicylic acid on morphological and Ornamental characteristics of petunia hybrida at drought stress”, Ind. J. Fund. Appl Life Sci vol. 4, no. 3, pp. 523-532, 2014.
14. [14] Y.Q. Zhao, C.L. Zhang, W. Zhang, L.N. Li, G.M. Zhang, “Molecular detection of Thielaviopsis basicola by PCR assay”, Acta Phytopat Sin, vol. 39, pp. 23–29, 2009.
15. [15] K. Miura, Y. Tada, “Regulation of water, salinity, and cold stress responses by salicylic acid”, Plant Physiol, vol.5, pp.1-12, 2014.
16. [16] S. Zeshuang, J. Guoying, L. Yingchun, Z. Yuxian, “Decrement of catalase mRNA level after salicylic acid treatment”, Chinese Sci Bull, vol.43, pp.4-12. 1998.
17. [17] A.A. Bahari, R. Sokhtesaraei, H.R. Chaghazardı, F. Masoudi, H. Nazarlı, “Effect of water deficit stress and foliar Application of salicylic acid on Antioxidants enzymes activity in leaves of Thymus daenensıs subsp. Lancifolius”, Cercetări Agronomice în Moldova, vol. XLVIII, no.1(161), pp. 57-67, 2015.