Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi

Öz

Söğüt mantarı olarak bilinen Lactarius controversus (Pers.) Pers., halk tarafından yenilebilir bir türdür. Dünyadaki birçok insan buruk tadı nedeniyle tüketmeyi tercih etmese de ülkemizde yaygın olarak tüketilmektedir. Bu çalışmada, L. controversus’un metanol ve etanol ekstraktlarının bazı mikroorganizmalara (Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa DMS50071, Bacillus megaterium DSM32, Staphylococcus aureus COWAN1, Candida albicans FMC17, Candida glabrata ATCC 66032 ve Trichophyton sp.) karşı antimikrobiyal etkileri araştırılmıştır. Disk difüzyon yöntemine göre L. controversus'un metanol ve etanol ekstraktlarının kullanılan mikroorganizmalara karşı antimikrobiyal (8.3-25.3 mm) etkiye sahip olduğu belirlenmiştir. Mikrodilüsyon yöntemine göre aynı türe ait metanol ve etanol ekstraktlarının minimum inhibitör konsantrasyon değerinin 31.25-250 µg/mL arasında olduğu tespit edilmiştir. Çalışma sonucunda L. controversus'un bakteri, maya ve dermatofitlere karşı antimikrobiyal ajan olarak kullanılabileceği belirlenmiştir.

Anahtar Kelimeler

• Yenilebilir mantarlar
• Lactarius controversus
• Antimikrobiyal etki
• Mikrodilüsyon yöntemi

Determination of antimicrobial effect of Lactarius controversus (Pers.) Pers

Öz

Lactarius controversus (Pers.) Pers., known as willow mushroom, is an edible species by the public. Although many people in the world do not prefer to consume it due to its acrid taste, it is widely consumed in our country. In this study, the antimicrobial effects of methanol and ethanol extracts of L. controversus against some microorganisms (Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa DMS50071, Bacillus megaterium DSM32, Staphylococcus aureus COWAN1, Candida albicans FMC17, Candida globrata ATCC 66032 and Trichophyton sp.) were investigated. According to the disk diffusion method, it has been determined that methanol and ethanol extracts of L. controversus have an antimicrobial effect (8.3-25.3mm) against microorganisms used. According to the microdilution method, it was determined that the minimum inhibitory concentration value of methanol and ethanol extracts of the same species was between 31.25-250 µg/mL. As a result of the study, it may be recommended to use L. controversus as an antimicrobial agent against bacteria, yeast, and dermatophytes.

Anahtar Kelimeler

• Edible mushrooms
• Lactarius controversus
• Microdilution method
• Antimicrobial effect

Kaynakça

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