In vitro evaluation of antimicrobial and antioxidant activity and GC/MS profiling of the endemic species Verbascum cheiranthifolium var. asperulum

Öz

Due to their potential therapeutic applications, there has been a growing fascination in the investigation of natural bioactive compounds from medicinal plants. Verbascum cheiranthifolium var. asperulum, an endemic species in Türkiye, has been studied for its potential antimicrobial and antioxidant characteristics. Various solvents were utilized for the extraction of plant material, and the antimicrobial activity was tested against various antibiotic-resistant bacterial strains and a yeast using the minimum inhibitory concentration (MIC) and agar disc diffusion methods. Additionally, the antioxidant potential was assessed using total phenolic content, the DPPH radical-scavenging assay, and total flavonoid content methods. The extracts have exhibites significant inhibitory effects against several bacterial strains, with the methanol extract showing the most potent antimicrobial activity. The most effective inhibition activity was observed against Staphylococcus aureus at a concentration of 1.56 mg/ml. Gas chromatography-mass spectrometry (GC-MS) analysis showed numerous phytochemical compounds, including phenols, alkaloids, flavonoids, and glycosides. The study highlights the potential of V. cheiranthifolium var. asperulum as a valuable and natural bioactive compound source with antimicrobial and antioxidant characteristics, making it a promising candidate for pharmaceutical, cosmetic, and food industries.

Anahtar Kelimeler

• Antimicrobial activity
• Antioxidant activity
• Chemical composition
• GC-MS

Endemik tür Verbascum cheiranthifolium var. asperulum'un antimikrobiyal ve antioksidan aktivitesinin in vitro değerlendirmesi ve GC/MS profil analizi

Öz

Son yıllarda, potansiyel terapötik uygulamaları nedeniyle tıbbi bitkilerden doğal biyoaktif bileşikler arayışına artan ilgi gözlenmektedir. Türkiye'ye endemik olan Verbascum cheiranthifolium var. asperulum, potansiyel antimikrobiyal ve antioksidan özellikleri için incelenmiştir. Bitki materyali farklı çözücüler kullanılarak ekstrakte edilmiş ve antimikrobiyal aktivite, agar disk difüzyon ve minimum inhibisyon konsantrasyonu (MIC) yöntemleri kullanılarak çeşitli antibiyotik dirençli bakteri suşlarına ve bir mayaya karşı test edilmiştir. Ayrıca antioksidan potansiyel, DPPH radikal yakalama testi, toplam flavonoid içeriği ve toplam fenolik içerik yöntemleri kullanılarak değerlendirilmiştir. Ekstraktlar birçok bakteri suşuna karşı önemli inhibe edici etkiler göstermiş, metanol özütünün en güçlü antimikrobiyal aktiviteye sahip olduğu gözlemlenmiştir. En etkili inhibisyon aktivitesi 1.56 mg/ml konsantrasyonunda Staphylococcus aureus’a karşı göstermiştir. Gaz kromatografi- kütle spektrometresi (GC-MS) analizi, fenoller, flavonoidler, alkaloitler ve glikozitler dâhil olmak üzere birçok fitokimyasal bileşiğin varlığını ortaya koymuştur. Bu çalışma, V. cheiranthifolium var. asperulum'un doğal antimikrobiyal ve antioksidan özelliklere sahip doğal biyoaktif bileşiklerin değerli bir kaynağı olarak potansiyelini vurgulamaktadır ve bu türü, farmasötik, kozmetik ve gıda endüstrileri için umut vadeden bir aday haline getirmektedir.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• Antioksidan aktivite
• Kimyasal bileşen
• GC-MS

Kaynakça

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