Türkiye'de yetişen Lallemantia Fish. & C.A. Mey. türlerinin çeşitli kanser hücre hatları üzerindeki sitotoksik etkisinin araştırılması

Öz

Lallemantia cinsi (Lamiaceae) halk arasında “Ajdarbaşı” olarak bilinir. Son derece zengin bileşiklere sahip olduğu kanıtlanmış olan cins, halk arasında birçok rahatsızlığın tedavisinde kullanılmaktadır. Dünyada 5 taksonla temsil edilen cinsin 3 taksonu Türkiye’de doğal yayılış göstermektedir. Bu çalışmada, Lallemantia peltata, Lallemantia iberica ve Lallemantia canescens taksonlarının toprak üstü kısımlarının metanolik ekstraktının BEAS-2B, SH-SY5Y, HCT 116 ve Hep G2 hücre hatlarına karşı sitotoksik özellikleri araştırılmıştır. Kanser hücrelerinin canlılığındaki değişiklikler ise, MTT yöntemi kullanılarak tespit edilmiştir. Sonuçlar, her üç türün de değişen oranlarda sitotoksik etkiye sahip olduğunu göstermiştir. Bu etki; 800 μg/ml'de HCT 116 üzerinde %53.54 (p<0.001), SH-SY5Y üzerinde %37.53 (p=0.005) ve Hep G2 üzerinde %32.03 (p=0.026) ile bu etkinin L. canescens'te maksimum ve L. iberica'da minimum olduğu gözlendi. Ayrıca bitkisel ekstraktların sağlıklı akciğer hücreleri (BEAS-2B) üzerinde ise herhangi bir toksik etki yaratmadığı gözlemlendi.

Anahtar Kelimeler

• Lallemantia
• Sitotoksik
• Beas-2B
• HCT116
• HepG2
• SH-SY5Y

Destekleyen Kurum

TUBITAK

Proje Numarası

TUBITAK-1919B012100464

Teşekkür

Bu çalışma TUBITAK tarafından 1919B012100464 numaralı proje olarak desteklenmiştir.

Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines

Öz

The genus Lallemantia (Lamiaceae) is popularly known as "Dragonhead". The genus, which has been proven to have extremely rich compounds, is used in the treatment of many diseases among the people. The genus is represented by 5 taxa in the world and 3 taxa are naturally distributed in Türkiye. In this study, the cytotoxic properties of methanolic extracts of the above-ground parts of Lallemantia peltata, Lallemantia iberica and Lallemantia canescens taxa were investigated against BEAS-2B, SH-SY5Y, HCT 116 and Hep G2 cell lines. Changes in the viability of cancer cells were determined using the MTT method. The results showed that all three species had varying degrees of cytotoxic effect. This effect was maximum in L. canescens with 53.54% (p<0.001) on HCT 116, 37.53% (p=0.005) on SH-SY5Y and 32.03% (p=0.026) on Hep G2 at 800 μg/ml and minimum in L. iberica. In addition, the herbal extracts had no toxic effect on healthy lung cells (BEAS-2B).

Anahtar Kelimeler

• Lallemantia
• Cytotoxic
• Beas-2B
• HCT116
• HepG2
• SH-SY5Y

Destekleyen Kurum

TUBITAK

Proje Numarası

TUBITAK-1919B012100464

Teşekkür

This study was supported by TUBITAK as project number 1919B012100464.

Kaynakça

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