Elektrokemoterapi meme kanseri tedavisinde Asparagus officinalis, Arum elongatum ve Urtica dioica özütlerinin etkinliğini arttırır

Öz

Bu çalışmanın amacı, farklı bölgelerden toplanan Asparagus officinalis L. bitkisinin gövdesinde, Arum elongatum Steven ve Urtica dioica L. bitkilerinin yapraklarında bulunan fenolik madde içeriklerini ve bunların MCF-7 insan meme kanseri hücre hattı üzerindeki sitotoksisitelerini belirlemek ve bu bitkilerin antiproliferatif aktiviteleri üzerine elektroporasyonun (EP) etkilerini ortaya koymaktır. Bitki ekstraktlarının antiproliferatif aktiviteleri MCF-7 insan meme kanseri hücrelerinde tespit edildi ve biyouyumlulukları MTT analiz yöntemi ile L-929 fibroblast hücrelerinde belirlendi. MCF-7 insan meme kanseri hücrelerinin elektrokemoterapi (ekstrakt+EP) uygulamalarında, bitki ekstraktlarının çeşitli dozları ile 800 V/cm şiddetinde sekiz kare dalga elektriksel darbe dizisi kullanıldı. Her üç bitki ekstraktının da fenolik bileşikler açısından zengin olduğu ve sadece A. elongatum'un L-929 fibroblast hücreleri üzerinde göreceli sitotoksik etki gösterdiği bulundu. Ancak MCF-7 kanser hücreleri, sırasıyla 443.57, 361.88 ve 448.55µg/mL IC50 değerleriyle A. officinalis, A. elongatum ve U. dioica özütlerinin sitotoksik aktivitelerine karşı çok iyi duyarlılık gösterdi. EP uygulamasıyla, üç bitki özütünün de kanser hücreleri üzerindeki sitotoksik aktivitesinin özüt uygulamasına kıyasla önemli ölçüde arttığı ve hücre canlılık yüzdelerinin önemli ölçüde azaldığı gözlendi. Sonuç olarak, bulgularımız A. officinalis, A. elongatum ve U. dioica özütlerinin antikanser potansiyeline sahip olduğunu ve EP ile kullanıldığında meme kanseri tedavisi için umut verici olabileceğini düşündürmektedir.

Anahtar Kelimeler

• Bitkisel tedavi
• Meme kanseri
• Elektroporasyon
• Fenolik maddeler

Electrochemotherapy enhances the efficacy of Asparagus officinalis, Arum elongatum and Urtica dioica extracts in breast cancer treatment

Öz

The aim of the current study was to determine the phenolic substances in the stem of Asparagus officinalis L, leaves of Arum elongatum Steven and Urtica dioica L. plants collected from different regions and their cytotoxicities on MCF-7 human breast cancer cell line and to reveal the effects of electroporation (EP) on the antiproliferative activities of these plants. Antiproliferative activities of plant extracts were determined in MCF-7 human breast cancer cells, and their biocompatibility was determined in L-929 fibroblast cells by MTT analysis method. In electrochemotherapy (extract+EP) applications of MCF-7 human breast cancer cells, eight square wave electrical pulse sequences with an intensity of 800 V/cm were used with various doses of plants extracts. It was found that all three plant extracts were rich in phenolic compounds and only A. elongatum showed a relative cytotoxic effect on L-929 fibroblast cells. However, MCF-7 cancer cells showed very good sensitivity to the cytotoxic activities of A. officinalis, A. elongatum and U. dioica extracts, with IC50 values of 443.57, 361.88, and 448.55µg/mL, respectively. It was observed that with EP application, the cytotoxic activity of all three plant extracts on cancer cells increased significantly compared to extract application and cell viability percentages decreased significantly. As a result, our findings suggest that A. officinalis, A. elongatum and U. dioica extracts have anticancer potential and may be promising for breast cancer when used with EP.

Anahtar Kelimeler

• Herbal treatment
• Breast cancer
• Electroporation
• Phenolic substances

Kaynakça

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