Artvin (Türkiye) kökenli Berberis vulgaris bitkisinin toprak üstü kısmının etanol ekstraktının bitkisel içerik analizi

Öz

İnsanlar eski çağlardan beri bitkileri beslenme, tedavi ve endüstriyel amaçlar gibi çeşitli alanlarda kullanmışlardır. Bitkiler çeşitli biyoaktif bileşiklerden ve ikincil metabolitlerden oluşur. Bu bileşikler ve metabolitler, ilaç ve biyoteknoloji endüstrisinde çeşitli amaçlarla etkin bir şekilde kullanılmaktadır. Türkiye, bitki çeşitliliği açısından ilaç etken maddesi olarak kullanılabilecek çok sayıda bitki florasına sahiptir. Bu bitkilerden biri olan Berberis vulgaris, kışın yapraklarını döken Berberidaceae ailesine ait dikenli bir çalıdır. Berberis vulgaris'in kimyasal içeriği farklı ekolojik koşullarına göre büyük ölçüde değişebilir. Bu nedenle, bu çalışma Türkiye'nin Artvin ili Şavşat ilçesinde yetiştiği bilinen Berberis vulgaris bitkisinin toprak üstü (yaprak ve dal) kısımlarından elde edilen etanol ekstraktındaki biyoaktif bileşiklerin belirlenmesi amacıyla gerçekleştirilmiştir. Analizin gerçekleştirilmesi için ilk adım olarak, B. vulgaris yaprak ve dal etanol ekstraksiyonu maserasyon yöntemi kullanılarak gerçekleştirildi. B. vulgaris bitkisinin kimyasal bileşenlerinin belirlenmesi için Gaz Kromatografisi Kütle Spektroskopisi yöntemi kullanılmış ve yaprakta 7, dalda ise 9 bileşik tanımlanmıştır. Oktatriakontil pentafloropropionat her iki özütte sırasıyla %59.026 ve %41.406 olarak belirlendi. Yaprak etanol ekstraktında belirlenen diğer bileşikler 9-oktadekenamid (%19.639), heptakosan (%6.582), α-tokoferol (%6.344), diizooktil ftalat (%3.166), fitol (%2.960), tetraetil silikat (%2.127) iken; Dal etanol ekstraktında belirlenen diğer bileşiklerde; erukamid (%27.679), diizooktil ftalat (%10.174), 2-hekzadekanol (%4.527), 8-oktadekenal (%4.243), hekza-t-bütil selenatrisiletan (%3.786), tetraetil silikat (%3.982), hekzadekanoik asit etil ester (%1.617), oleik asit, 3-(oktadesiloksi)propil ester (%2.585) idi.

Anahtar Kelimeler

• Berberis vulgaris
• Etanol ekstraksiyonu
• Gaz Kromatografisi
• Kütle Spektroskopisi

Proje Numarası

No

Teşekkür

We thank Atatürk University Faculty of Pharmacy for GC-MS analyses of the study.

Herbal content analysis of ethanol extract of aerial part of Berberis vulgaris plant originating from Artvin (Türkiye)

Öz

Since ancient times, people have used plants in various areas such as nutrition, treatment and industrial purposes. Plants consist of various bioactive compounds and secondary metabolites. These compounds and metabolites are effectively used for various purposes in the pharmaceutical and biotechnological industry. Türkiye has a large number of plant flora that can be used as active pharmaceutical ingredients in terms of plant diversity. One of these plants, Berberis vulgaris, is a thorny shrub belonging to the Berberidaceae family that sheds its leaves in winter. The chemical composition of Berberis vulgaris is susceptible to considerable variation, contingent upon divergent ecological factors. Consequently, this study was conducted to ascertain the bioactive compounds present in the ethanol extract obtained from the aboveground (leaf and branch) parts of the Berberis vulgaris plant, which is known to be cultivated in the Şavşat district of Artvin province, Türkiye. As a first step to carry out the analysis, ethanol extraction of B. vulgaris leaves and branches was carried out using the maceration method. Gas chromatography-mass spectrometry was used to determine the chemical constituents of B. vulgaris, and 7 compounds were identified in the leaf and 9 compounds in the branch. Octatriacontyl pentafluoropropionate was determined as 59.026% and 41.406% in both extracts, respectively. Other compounds determined in the leaf ethanol extract were 9-octadecenamide (19.639%), heptacosane (6.582%), α-tocopherol (6.344%), diisooctyl phthalate (3.166%), phytol (2.960%), tetraethyl silicate (2.127%); Other compounds determined in the branch ethanol extract were erucamide (27.679%), diisooctyl phthalate (10.174%), 2-hexadecanol (4.527%), 8-octadecenal (4.243%), hexa-t-butyl selenatrisiletane (3.786%), tetraethyl silicate (3.982%), hexadecanoic acid ethyl ester (1.617%), oleic acid, 3-(octadecyloxy)propyl ester (2.585%).

Anahtar Kelimeler

• Berberis vulgaris
• Ethanol extraction
• Gas Chromatography
• Mass Spectroscopy

Proje Numarası

No

Kaynakça

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