Biological activity potential of Thalictrum orientale: antioxidant, anticholinesterase, and antiproliferative effects

Öz

Plants are among the important natural resources in terms of biological activity. In this study, the biological activity of Thalictrum orientale species was evaluated comprehensively. For this purpose, ethanol extract of the plant was obtained by Soxhlet extraction method. To determine the antioxidant capacity, total antioxidant status (TAS) and total oxidant status (TOS) were analyzed using Rel Assay kits. Anticholinesterase activity was determined by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. In addition, antiproliferative activity was evaluated on A549 lung cancer cell line. As a result of the analyzes, TAS value of Thalictrum orientale was determined as 5.413±0.149 mmol/L, TOS value as 8.300±0.056 µmol/L and oxidative stress index (OSI) as 0.154±0.004. Within the scope of anticholinesterase activity, AChE inhibition value was determined as 35.41±1.88 µg/mL and BChE inhibition value was determined as 58.63±1.24 µg/mL. It was observed that the antiproliferative effect increased in direct proportion to the increase in concentration. The findings show that Thalictrum orientale has high biological potential in terms of antioxidant, antiproliferative and anticholinesterase activities.

Anahtar Kelimeler

• Thalictrum orientale
• antioxidant activity
• anticholinesterase activity
• antiproliferative activity
• biological activity

Thalictrum orientale'nin biyolojik aktivite potansiyeli: antioksidan, antikolinerjik ve antiproliferatif etkiler

Öz

Bitkiler, biyolojik aktivite açısından önemli doğal kaynaklar arasında yer almaktadır. Bu çalışmada, Thalictrum orientale türünün biyolojik aktivitesi kapsamlı bir şekilde değerlendirilmiştir. Bu amaçla, bitkinin etanol özütü Soxhlet ekstraksiyon yöntemiyle elde edilmiştir. Antioksidan kapasitesinin belirlenmesi için toplam antioksidan statusu (TAS) ve toplam oksidan statusu (TOS) Rel Assay kitleri kullanılarak analiz edilmiştir. Antikolinesteraz aktivitesi, asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) enzim aktiviteleri ölçülerek belirlenmiştir. Ayrıca, antiproliferatif aktivite, A549 akciğer kanser hücre hattı üzerinde değerlendirilmiştir. Yapılan analizler sonucunda Thalictrum orientale’in TAS değeri 5.413±0.149 mmol/L, TOS değeri 8.300±0.056 µmol/L ve oksidatif stres indeksi (OSI) 0.154±0.004 olarak belirlenmiştir. Antikolinesteraz aktivitesi kapsamında AChE inhibisyon değeri 35.41±1.88 µg/mL, BChE inhibisyon değeri ise 58.63±1.24 µg/mL olarak saptanmıştır. Antiproliferatif etkinin, konsantrasyon artışı ile doğru orantılı olarak arttığı gözlemlenmiştir. Elde edilen bulgular, Thalictrum orientale’in antioksidan, antiproliferatif ve antikolinesteraz aktiviteleri açısından yüksek biyolojik potansiyele sahip olduğunu göstermektedir.

Anahtar Kelimeler

• Thalictrum orientale
• antioksidan aktivite
• antiproliferatif aktivite
• biyolojik aktivite
• Antikolinesteraz aktivite

Kaynakça

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