Synergistic Effects of Liquid Seaweed Fertilizer and Mycorrhiza on Barley Growth and Rhizosphere Biological Properties

Öz

Seaweed fertiliser is widely used in agriculture as a nutrient supplement, biofertiliser and biostimulant for soil. Mycorrhiza increases nutrient uptake, disease resistance, drought resistance and yield of plants. The aim of this study was to evaluate the effects of liquid seaweed fertiliser and mycorrhiza separately and in combination on the growth of barley seedlings and some enzymatic activities of the rhizosphere. Liquid seaweed fertiliser (0 (conrol), 0.4, 0.6, 1, 1.5 g/l) was used in five different doses, mycorrhiza (with mycorrhiza, without mycorrhiza) was used in two different doses and combinations of both biostimulants were applied to the soil. The addition of liquid seaweed fertiliser (1 g/l) and mycorrhiza combination to the soil significantly increased plant height and root length of barley seedlings. Especially, 1 g/l seaweed fertiliser x mycorrhiza and 1.5 g/l seaweed fertiliser x mycorrhiza treatments increased the amount of chlorophyll a and b in leaves compared to other treatments. Co-application of increasing doses of seaweed fertiliser and mycorrhiza increased dehydrogenase, alkaline phosphatase, β-glucosidase and catalase enzyme activities of rhizosphere soil. With the results obtained, it was revealed that the application of 1 g/l and 1.5 g/l doses of seaweed fertiliser to the soil together with mycorrhiza can improve the enzyme activities of the soil, thus supporting the view that soil quality will increase with the increase of microorganisms population. These changes in the soil may encourage seedling growth and contribute to the increase in plant production.

Anahtar Kelimeler

• Mycorrhiza
• Seaweed
• Dose
• Soil enzyme activity

Sıvı Yosun Gübresi ve Mikorizanın Arpa Gelişimi ve Rizosferin Biyolojik Özelliklerine Sinerjik Etkileri

Öz

Yosun gübresi tarımda besin takviyesi, biyogübre ve toprak için biyostimülant olarak yaygın şekilde kullanılmaktadır. Mikoriza ise bitkilerin besin alımını, hastalık direncini, kuraklığa dayanıklılığını, bitkilerin verimini artırmaktadır. Bu çalışmanın amacı, sıvı yosun gübresi ve mikorizanın ayrı ayrı ve kombinasyonlarının arpa fidelerinin büyümesi ve rizosferin bazı enzimatik aktiviteleri üzerine etkilerini değerlendirmektir. Sıvı yosun gübresi beş farklı (0 (kontrol), 0.4, 0.6, 1, 1.5 g/l) dozda, mikoriza (mikorizalı, mikorizasız) iki farklı dozda kullanılmış ve her iki biyostimülantın kombinasyonları da toprağa uygulanmıştır. Sıvı yosun gübresi (1 g/l) ve mikoriza kombinasyonunun toprağa eklenmesi, arpa fidelerinin bitki boyu ve kök uzunluğunu önemli ölçüde artırmıştır. Özellikle 1g/l yosun gübresi x mikoriza ve 1.5 g/l yosun gübresi x mikoriza uygulamaları diğer uygulamalarla karşılaştırıldığında yaprakların klorofil a ve b miktarını artırmıştır. Artan dozlarda yosun gübresi ile mikorizanın birlikte uygulanması rizosfer toprağının dehidrogenaz, alkalin fosfataz, β-glukosidaz ve katalaz enzim aktivitelerinde artış göstermiştir. Elde edilen sonuçlar ile özellikle yosun gübresinin 1 g/l’lik ve 1.5 g/l dozunun mikoriza ile birlikte toprağa uygulanmasının toprağın enzim aktivitelerini iyileştirebildiği ortaya konmuş dolayısıyla mikroorganizma popülasyonunun artmasıyla toprak kalitesinin artacağı görüşü desteklenmiştir. Toprakta oluşacak bu değişiklikler fide büyümesini teşvik edebilir, bitkisel üretimin artmasına katkı sağlayabilir.

Anahtar Kelimeler

• Mikoriza
• Deniz yosunu
• Doz
• Toprak enzim aktivitesi

Kaynakça

1. Agarwal P, Gupta R, Gill IK (2018) Importance of biofertilizers in agriculture biotechnology. Annals of Biological Research, 9(3):1–3.
2. Anlı M, El kaoua M, Ait El Mokhtar M, Boutasknit A, Ben-laouane R, Toubali S, Baslam M, Lyamlouli K, Hafidi M, Meddich A (2020) Seaweed extract application and arbuscular mycorrhizal fungal inoculation: a tool for promoting growth and development of date palm (Phoenix dactylifera L.) cv “Boufgous”. South African Journal of Botany, 132:15–21. https://doi.org/10.1016/j.sajb.2020.04.004
3. Arnon DT (1967) Copper Enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant Physiology, 24:1-15. https://doi.org/10.1104/pp.24.1.1
4. Ashour M, Hassan SM, Elshobary ME, Ammar GA, Gaber A, Alsanie WF, El-Shenody R (2021) Impact of commercial seaweed liquid extract (TAM®) biostimulant and its bioactive molecules on growth and antioxidant activities of hot pepper (Capsicum annuum). Plants, 10(6):1045. https://doi.org/10.3390/plants10061045
5. Basavaraja PK, Yogendra ND, Zodape ST, Prakash R, Ghosh A (2018) Effect of seaweed sap as foliar spray on growth and yield of hybrid maize. Journal of Plant Nutrition, 41:1851–1861. https://doi.org/10.1080/01904167.2018.1463381
6. Basiru S, Hijri M (2024) Trade-off between soil organic carbon sequestration and plant nutrient uptake in arbuscular mycorrhizal symbiosis. Fungal Biology Reviews, 49:100381. https://doi.org/10.1016/j.fbr.2024.100381
7. Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B (2015) Seaweed extracts as biostimulants in horticulture. Scientia Horticulturae, 196:39-48. https://doi.org/10.1016/j.scienta.2015.09.012
8. Beck TH (1971) The determination of catalase activity in soils. Journal of Plant Nutrition and Soil Science, 130:68–81. https://doi.org/10.1002/jpln.19711300108

9. Bona E, Cantamessa S, Massa N, Manassero P, Marsano F, Copetta A, Lingua G, D’Agostino G, Gamalero E, Berta G (2017) Arbuscular mycorrhizal fungi and plant growth-promoting pseudomonads improve yield, quality and nutritional value of tomato: a field study. Mycorrhiza, 27:1–11. https://doi.org/10.1007/s00572-016-0727-y
10. Colla G, Cardarelli M, Bonini P, Rouphael Y (2017) Foliar applications of protein hydrolysate, plant and seaweed extracts increase yield but differentially modulate fruit quality of greenhouse tomato. HortScience, 52:1214–1220. https://doi.org/10.21273/HORTSCI12200-17
11. Craigie JS (2011) Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology,23:371-393. https://doi.org/10.1007/s10811-010-9560-4
12. Cüre B (2022) Kimyasal ve organik gübrelerin çevre üzerine etkisi. Biyosistem Mühendisliği Dergisi, 3(2): 98-107.
13. Di Mola I, Cozzolino E, Ottaiano L, Giordano M, Rouphael Y, Colla G, Mori M (2019) Effect of vegetal and seaweed extract-based biostimulants on agronomical and leaf quality traits of plastic tunnel-grown baby lettuce under four regimes of nitrogen fertilization. Agronomy, 9(10):571. https://doi.org/10.3390/agronomy9100571
14. Du Jardin P (2015) Plant biostimulants: definition, concept, main categories and regulation. Science Horticulture, 196:3–14. https://doi.org/10.1016/j.scienta.2015.09.021
15. Güler A, Küçük Ç (2024) Sıvı yosun gübresinin farklı dozlarının tuz stresi koşullarında arpa gelişimi ve rizosferdeki bazı biyolojik özelliklere etkisi. Mus Alparslan University Journal of Science, 12(1):51-57. https://doi.org/10.18586/msufbd.1403059
16. Hassan SM, Ashour M, Sakai N, Zhang L, Hassanien HA, Gaber A, Ammar G (2021) Impact of seaweed liquid extract biostimulant on growth, yield and chemical composition of cucumber (Cucumis sativus). Agriculture, 11(4):320. https://doi.org/10.3390/agriculture11040320
17. JMP Statistical Software (2020) JMP 1989-2007. Version 5.0. SAS Institute Inc: Cary, NC, USA. https://www.capterra.com/p/151815/JMP-Statistical-Software
18. Kaur S, Suseela V (2020) Unraveling arbuscular mycorrhiza-induced changes in plant primary and secondary metabolome. Metabolites, 10:1-30. https://doi.org/10.3390/metabo10080335
19. Kinany SE, Achbani E, Faggroud M, Ouahmane L, Hilali RE, Haggoud A, Bouamri R (2019) Effect of organic fertilizer and commercial arbuscular mycorrhizal fungi on the growth of micropropagated date palm cv. Feggouss. Journal of the Saudi Society of Agricultural Sciences, 18:411–417. https://doi.org/10.1016/J.JSSAS.2018.01.004
20. Kulkarni MG, Rengasamy KR, Pendota SC, Gruz J, Plackova L, Novak O (2019) Bioactive molecules derived from smoke and seaweed Ecklonia maxima showing phytohormone-like activity in Spinacia oleracea L. New Biotechnology, 48:83–89. https://doi.org/10.1016/j.nbt.2018.08.004
21. Küçük Ç, Uslu P, Sezen G (2024) Cladophora sp. ve Arbüsküler Mikorizal Fungus (AMF) spor aşılamasının mısır bitkisinin (Zea mays L.) gelişim parametreleri ve bazı rizosfer toprak enzimlerine etkisi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 28(2): 189-196. https://doi.org/10.19113/sdufenbed.1473028
22. Küçük Ç, Demir M (2025) Yosun gübresi ve mikoriza uygulanmasının mercimek bitkisi rizosfer bölgesinin biyolojik aktivitesine etkileri. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 15(1): 11-20. https://doi.org/10.17714/gumusfenbil.1444495
23. Liu CY, Zhang F, Zhang DJ, Srivastava AK, Wu QS, Zou YN (2018) Mycorrhiza stimulates root-hair growth and IAA synthesis and transport in trifoliate orange under drought stress. Science Reports, 8:1–9. https://doi.org/10.1038/s41598-018-20456-4
24. Mendez A, Martinez S, Leal A, Hernandez A, Garcia J, Sanchez M (2023) Synergism of microorganisms and seaweed extract on vegetative growth, yield and quality of cucumber fruit. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51:12888. https://doi.org/10.15835/nbha51312888
25. Mukherjee A, Gaurav AK, Patel AK, Singh S, Chouhan GK, Lepcha A (2021) Unlocking the potential plant growth-promoting properties of chickpea (Cicer arietinum l.) seed endophytes bio-inoculants for improving soil health and crop production. Land Degradation & Development, 32(15):4362–4374. https://doi.org/10.22541/au.161518112.26081982/v1
26. Ngoroyemoto N, Kulkarni MG, Stirk WA, Gupta S, Finnie JF, van Staden J (2020) Interactions between microorganisms and a seaweed-derived biostimulant on the growth and biochemical composition of Amaranthus hybridus L. Natural Product Communications, 15(7):1-11. https://doi.org/10.1177/1934578X20934228
27. Oral E, Kendal E, Doğan Y (2017) Adıyaman ve Şanlıurfa-Hilvan şartlarında yazlık arpa genotiplerinde verim ve bazı kalite kriterlerinin araştırılması. Journal of Agricultural Faculty of Uludag University, 31(2):23-36.
28. Oyetunji OJ, Ekanayake IJ, Osonubi O (2007) Chlorophyll fluorescence analysis for assessing water deficit and arbuscular mycorrhizal fungi (AMF) inoculation in cassava (Manihot esculenta Crantz). Advances in Biological Research, 1(3-4): 108-117.
29. Pepper IL, Gerba CP, Brendecke JW (1995) Environmental Microbiology: A Laboratory Manual. Academic Press Inc. New York, USA.
30. Prasedya ES, Kurniawan NSH, Kirana IAP, Ardiana N, Abidin AS, Ilhami BTK, Jupri A, Widyastuti S, Sunarpi H, Nikmatullah A (2022) Seaweed fertilizer prepared by EM-Fermentation increases abundance of beneficial soil microbiome in paddy (Oryza sativa L.) during vegetative stage. Fermentation, 8:1-15. https://doi.org/10.3390/fermentation8020046
31. Prasedya ES, Kurniawan NSH, Fitriani F, Saraswati PBA, Qoriasmadillah W, Ilhami BTK, Hernawan A, Widyastuti S (2023) Sustainable use of organic seaweed fertilizer improves the metagenomic function of microbial communities in the soil of rice plants. Sustainability, 15(23):16328. https://doi.org/10.3390/su152316328
32. Rasouli F, Amini T, Asadi M, Hassanpouraghdam MB, Aazami MA, Ercisli S, Skrovankova S, Mlcek J (2022) Growth and antioxidant responses of lettuce (Lactuca sativa L.) to arbuscular mycorrhiza inoculation and seaweed extract foliar application. Agronomy, 12(2):401. https://doi.org/10.3390/agronomy12020401
33. Rouphael Y, Colla G (2020) Biostimulants in agriculture. Frontiers in Plant Science, 11:1-7. https://doi.org/10.3389/fpls.2020.00040
34. Ruiz-Sánchez M, Aroca R, Muñoz Y, Polón R, Ruiz-Lozano JM (2010) The arbuscular mycorrhizal symbiosis enhances the photosynthetic efficiency and the antioxidative response of rice plants subjected to drought stress. Journal of Plant Physiology, 167:862–869. https://doi.org/10.1016/j.jplph.2010.01.018
35. Sharma S, Chen C, Khatri K, Rathore MS, Pandey SP (2019) Gracilaria dura extract confers drought tolerance in wheat by modulating abscisic acid homeostasis. Plant Physiology and Biochemistry, 136:143–154. https://doi.org/10.1016/j.plaphy.2019.01.015
36. Shukla PS, Mantin EG, Adil M, Bajpai S, Critchley AT, Prithiviraj B (2019) Ascophyllum nodosum-based biostimulants: sustainable applications in agriculture for the stimulation of plant growth, stress tolerance, and disease management. Frontiers Plant Science, 10:655. https://doi.org/10.3389/fpls.2019.00655
37. Sivakumar PV, Palanisamy K, Lenin M (2020) Potential role of arbuscular mycorrizhal fungi (AMF) and vermicompost (VC) on the maturation of agriculture crops. South Asian Journal of Life Sciences, 8(2):24-37. https://doi.org/10.17582/journal.sajls/2020/8.2.24.37
38. Subbiah V, Xie C, Dunshea FR, Barrow CJ, Suleria HA (2023) The quest for phenolic compounds from seaweed: nutrition, biological activities and applications. Food Reviews International, 39(8):5786-5813. https://doi.org/10.1080/87559129.2022.2094406
39. Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1:301-307. https://doi.org/10.1016/0038-0717(69)90012-1
40. Tarraf W, Ruta C, Tagarelli A, De Cillis F, De Mastro G (2017) Influence of arbuscular mycorrhizae on plant growth, essential oil production and phosphorus uptake of Salvia officinalis L. Industrial Crops and Products, 102:144–153. https://doi.org/10.1016/j.indcrop.2017.03.010
41. Tujula NA, Crocetti GR, Burke C, Thomas T, Holmstrom C, Kjelleberg S (2010) Variability and abundance of the epiphytic bacterial community associated with a green marine Ulvacean alga. The ISME Journal, 4(2):301-311. https://doi.org/10.1038/ismej.2009.107
42. Türkmen M, Su A (2019) The effect of sea lettuce (Ulva lactuca) liquid fertilizer and zeolite combinations on the development of cucumber (Cucumis sativus). Turkish Journal of Agriculture Food Science and Technology, 7:1021–1027. https://doi.org/10.24925/turjaf.v7i7.1021-1027.2520
43. uz Zaman Q, Guo L, He X, Luo Y, Liu C, Murtaza G, Sultan K, Fahad S, Cheng X, Ashraf K, Deng G (2025) Synergistic effect of arbuscular mycorrhizal fungi, biochar and seaweed extract for improving the copper tolerance in hemp. Industrial Crops and Products, 224:120358. https://doi.org/10.1016/j.indcrop.2024.120358
44. Vafa Z, Najafi Y, Sohrabi R, Sayyed Z, Luh Suriani N, Datta R (2021) Effects of the combinations of rhizobacteria, mycorrhizae, and seaweed, and supplementary irrigation on growth and yield in wheat cultivars. Plants, 10:811. https://doi.org/10.3390/plants10040811
45. Vafa NZ, Sohrabi Y, Mirzaghaderi G, Heidari G (2022) Soil microorganisms and seaweed application with supplementary irrigation improved physiological traits and yield of two dryland wheat cultivars. Frontiers Plant Science, 13:855090. https://doi.org/10.3389/fpls.2022.855090
46. Varma A, Prasad R, Tuteja N (2018) Mycorrhiza-Nutrient uptake, Biocontrol, Ecorestoration. Springer: Berlin/Heidelberg, Germany, 1–539.
47. Wang MP, Chen L, Li YT, Chen L, Liu ZY, Wang XJ, Yan PS, Qin S (2018) Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing. Applied Soil Ecology, 125:288-296. https://doi.org/10.1016/j.apsoil.2018.02.013
48. Wipf D, Krajinski F, van Tuinen D, Recorbet G, Courty PE (2019) Trading on the arbuscular mycorrhiza market: from arbuscules to common mycorrhiza networks. New Phytology, 223:1127–1142. https://doi.org/10.1111/nph.15775
49. Xiao Y, Liu M, Chen L, Ji L, Zhao Z, Wang L (2020) Growth and elemental uptake of Trifolium repens in response to biochar addition, arbuscular mycorrhizal fungi and phosphorus fertilizer applications in low-Cd-polluted soils. Environmental Pollution, 260:1–9. https://doi.org/10.1016/j.envpol.2019.113761
50. Xie X, Lai W, Che X, Wang S, Ren Y, Hu W, Tang M (2022) A SPX domain-containing phosphate transporter from Rhizophagus irregularis handles phosphate homeostasis at symbiotic interface of arbuscular mycorrhizas. New Phytologist, 234:17973. https://doi.org/10.1111/nph.17973
51. Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH (2017) Biostimulants in plant science: a global perspective. Frontiers Plant Science, 7:2049. https://doi.org/10.3389/fpls.2016.02049
52. Yıldırım F, Cevheri C, Küçük Ç (2024) Effect of different mycorrhizal inoculations and phosphorus doses on vetch (Vicia sativa) growth under greenhouse conditions. Commagene Journal of Biology, 8(1): 24-29. https://doi.org/10.31594/commagene.1449187
53. Zhang Z, Chen Q, Yin C, Shen X, Chen X, Sun H, Mao Z (2012) The effects of organic matter on the physiological features of Malus hupehensis seedlings and soil properties under replant conditions. Scientia Horticulturae, 146:52-58. https://doi.org/10.1016/j.scienta.2012.08.011
54. Zhang F, Liu M, Li Y, Che Y, Xiao Y (2019) Effects of arbuscular mycorrhizal fungi, biochar and cadmium on the yield and element uptake of Medicago sativa. Science of the Total Environment, 655:1150–1158. https://doi.org/10.1016/j.scitotenv.2018.11.317