Thuja occidentalis (AFS-Thuja) Bitkisinin Antioksidan Kapasitesi, Bazı Fitokimyasalları ve Antimikrobiyal Etkilerinin Incelenmesi

Öz

Birçok bitki, insanlar tarafından çok eski çağlardan beri çeşitli hastalıkların tedavisinde kullanılmaktadır. Thuja occidentalis de bu bitkilerden biridir ve ilk olarak Kanada’daki yerli halk tarafından iskorbüt hastalığının tedavisinde kullanılmıştır. Hastalıklara karşı iyileştirici etkileri bilinen bu bitki, genellikle Kuzeydoğu Amerika’da yayılış göstermektedir. Bu özelliği nedeniyle bilim insanlarının dikkatini çekmiş ve kurutulmuş kısımlarının ana bileşenleri incelenmiştir. Bu çalışmada, Thuja occidentalis’in antioksidan aktivitesi, uçucu bileşenleri, fenolik bileşenleri ve antimikrobiyal etkileri araştırılmıştır. Antioksidan aktivite kapsamında DPPH radikal süpürme aktivitesi IC₅₀ değeri 1,83 olarak belirlenmiştir. Uçucu bileşenler içerisinde en yüksek miktara sahip bileşikler; aldehitler grubunda p-Cumic aldehyde, ketonlar grubunda Carvotanaceton, asitler grubunda Hexanoic acid, esterler grubunda Nonanoic acid methyl ester ve terpenler arasında D-Carvone olarak tespit edilmiştir. Fenolik bileşenler içerisinde ise Kateşin (28,320 mg/10 g ekstrakt) metanol ve aseton ekstraktlarında en yüksek miktarda bulunan bileşik olmuştur. Ayrıca T. occidentalis bitkisinin Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 ve Candida albicans ATCC 10231 suşları üzerinde antimikrobiyal etkisi incelendiğinde, her iki çözücüde de önemli aktivite gösterdiği ve konsantrasyona bağlı olarak 14–31 mm arasında değişen inhibisyon zonları oluşturduğu belirlenmiştir.

Anahtar Kelimeler

• Thuja occidentalis
• Uçucu Bileşenler
• Antioksidan
• Antimikrobiyal

Investigation of Antioxidant Capacity, Some Phytochemicals and Antimicrobial Effects of Thuja occidentalis (AFS-Thuja) Plant

Öz

Plants have been utilized by humans since ancient times for the treatment of various diseases. Among these, Thuja occidentalis has been reported to be traditionally used by indigenous populations in Canada for the treatment of scurvy. This plant, known for its therapeutic properties against a range of diseases, is predominantly distributed in Northeastern America. Owing to these characteristics, it has attracted considerable scientific interest, and the major constituents of its dried parts have been extensively investigated. Accordingly, the present study aims to evaluate the antioxidant activity, as well as the volatile and phenolic compositions, and the antimicrobial properties of Thuja occidentalis. The antioxidant activity, as determined by the DPPH radical scavenging assay, yielded an IC₅₀ value of 1.83. Among the volatile compounds, the most abundant constituents were identified as p-Cumic aldehyde in aldehydes, Carvotanaceton in ketones, Hexanoic acid in acids, Nonanoic acid methyl ester in esters, and D-carvone in terpenes. Among the phenolic compounds, catechin (28.320 mg/10 g extract) was found to be the predominant component in both methanolic and acetone extracts. Furthermore, the antimicrobial activity of T. occidentalis was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans strains. The results indicated that the plant exhibited significant antimicrobial effects in both solvents, with inhibition zone diameters ranging from 14 mm to 31 mm in a concentration-dependent manner.

Anahtar Kelimeler

• Thuja occidentalis
• Volatile Components
• Antioxidant
• Antimicrobial

Kaynakça

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