Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi

Öz

Bu çalışma, Vinca major subsp. hirsuta’nın fenolik içeriğinin ve antioksidan aktivitesinin belirlenmesi amacıyla yürütülmüştür. Bitkinin fenolik içeriğinin belirlenmesi için ters fazlı yüksek performanslı sıvı kromatografisi (RP-HPLC-UV) kullanılmıştır. Toplam fenolik madde, FRAP ve DPPH radikal temizleme yöntemleriyle bitkinin antioksidan aktivitesi belirlenmiştir. Bitki yaprak, çiçek ve sap olmak üzere üç kısma ayrılmıştır. Uygulanan antioksidan yöntemlere göre bitkinin yaprak kısmı en yüksek aktivite göstermiştir. RP-HPLC-UV ile Vinca major subsp. hirsuta.’ın tüm kısımlarında ve ekstraktlarında değişik konsantrasyonlardaki 5 farklı fenolik bileşen tespit edilmiştir. Klorojenik asit 5344,93 ila 112,97 µg phenolic bileşen/g kuru örnek birimle ana bileşen olarak belirlenmiştir. Üç örnekte de en düşük fenolik bileşen gallik asit olarak bulunmuştur. Bitkinin üç parçasında en yüksek fenolik bileşen içeriğini ise yaprak göstermiştir.

Anahtar Kelimeler

• Antioksidan,HPLC; Fenolikler; Vinca major ssp. hirsuta

Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV

Öz

The aim of the study is to determine phenolic composition and antioxidant activity from Vinca major subsp. hirsuta. Reverse phase high performance liquid chromatography (RP-HPLC-UV) was used to determination phenolic compounds. The antioxidant activity of the plant was investigated in terms of total phenolic content, FRAP, and DPPH radical scavenging assays. The plant is divided into three parts: leaf, flower and stem. According to the applied antioxidant assays, leaf of the plant showed the highest activity. 5 different phenolic compounds in the range of concentrations were determined in the all extracts and parts of Vinca majör subsp. hirsuta by RP-HPLC-UV. Chlorogenic acid was the main ranging from 5344.93 to 112.97 μg phenolic compound/g dry sample, respectively. Gallic acid was found to be lowest in the three parts of the samples. The leaf was observed highest phenolic compounds between the three samples.

Anahtar Kelimeler

• Antioxidant,HPLC,Phenolics,Vinca major subsp. hirsuta

Kaynakça

1. Aljadi A M, Kamaruddin M Y (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys, Food Chem, 85: 513–518
2. Aruoma O I (1998) Free radicals, oxidative stress, and antioxidants in human health and disease, J Am Oil Chem Soc, 75: 199-212
3. Atta-Ur-Rahman, Sultana A, Nighat F, Bhatti M K, Kartal M, Kurucu S (1995) Alkaloids from vinca majör, Phytochemistry, 38: 1057-1061
4. Baytop T (1984) Türkiye’de Bitkiler ile Tedavi (Geçmişte ve Bugün), İstanbul, İstanbul Üniversitesi Yayınları, pp. 423
5. Benzie I, Strain J J (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay, Anal Biochem, 239: 70-76
6. De Villiers A, Lynen F, Crouch A, Sandra P (2004) Development of a solid-phase extraction procedure for the simultaneous determination of polyphenols, organic acids and sugars in wine, Chromatographia, 59: 403–409
7. Direosti I E (2000) Antioxidant polyphenols in tea, cocoa, and wine, Nutrition, 16: 692-694
8. Evans W C (1994) Trease and Evans' Pharmacognosy. London: Bailliere Tindall and Cansell Ltd.

9. Gilkey H (1957) Weeds of the Pacific Northwest, Oregon State College, OR
10. Grace S C, Logan B A (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species, Plant Physiol, 112: 1631-1640
11. Gülçin İ, Büyükokuroğlu M E, Oktay M, Küfrevioğlu Ö İ (2003) Antioxidant and analgesic activities of turpentine of Pinus nigra arn. subsp. palisiana (Lamb.) Holmboe, J Ethnopharmacol, 86: 51–58
12. Halliwell B, Aruoma O I (1997) “Free radicals and antioxidants: The need for in vivo markers of oxidative stress”. Aruoma, O I, Cuppett, S L (Eds.), Antioxidant Methodology: in vivo and in vitro Concepts, AOCS Press, Champaign, Illinois. pp. 1-22
13. Halliwell B, Gutteridge J M C (2000) Free radicals in biology and medicine, Third ed. Oxford: Oxford Science Publications, p. 617–24
14. Halliwell B (2000) The Antioxidant Paradox, Lancet, 355: 1179–1180
15. Halliwell B (2003) Oxidative stress in cell culture: An under-appreciated problem, FEBS Lett, 540: 3–6
16. Han X, Shen T, Lou H (2007) Dietary polyphenols and their biological significance, Int J Mol Sci, 8: 950-988
17. Havsteen B H (2002) The biochemistry and medical significance of the flavonoids, Pharmacol Ther, 96: 67–202
18. Hesse M (2002) Alkaloids. Nature’s Curse or Blessing? Wiley-VCH, Weinheim
19. Il'yashenko L I, Malikov V M, Yagudaev M R, Yunusov S Y (1977) Alkaloids of Vinca majör, Chem Nat Comp, 13: 324-327
20. Johnston K L, Clifford M N, Morgan L M (2003) Coffee acutely modifies gastrointestinal hormone secretion and glucose tolerance in humans: glycemic effects of chlorogenic acid and caffeine, Am J Clin Nutr, 79: 728–733
21. Kolayli S, Küçük M, Duran C, Candan F, Dincer B (2003) Chemical and antioxidant properties of Laurocerasusu officinalis Roem.(Cherry Laurel) fruit grown in the Black Sea region, J Agric Food Chem 51: 7489-7494
22. Kolaylı S, ¸Sahin H, Ulusoy E, Tarhan Ö (2010) Phenolic composition and antioxidant capacities of Helichrysum plicatum, Hacettepe J Biol Chem 38: 269–276
23. Kweon M, Hwang H, Sung H (2001) Identification and Antioxidant Activity of Novel Chlorogenic Acid Derivatives from Bamboo (Phyllostachys edulis), J Agr Food Chem, 49: 4646–46552
24. Logan B A, Demmig-Adams B, Adams W W, Grace S C (1998) Antioxidants and xanthophyll cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field, J Exp Bot, 49: 1869-1879
25. Mehrab S, Majd A, Tamadon T (1995) The antimicrobial effect of genus vinca on some pathogen microorganism, Iran J Public Health, 24: 7-14
26. Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin J Sci Technol, 26: 211-219
27. Özturk N, Tuncel M, Tuncel N B (2007) Determination of phenolic acids by a modified HPLC: Its application to various plant materials, J Liq Chromatogr Relat Technol, 30:587-596
28. Peterson J, Dwyer J (1998) Flavonoids: dietary occurrence and biochemical activity, Nutr Res, 18: 1995–2018
29. Rajput M S, Nair V, Chauhan A, Jawanjal H (2011) Dange, Evaluation of Antidiarrheal Activity of Aerial Parts of Vinca major in Experimental Animals, Middle-East J Sci Res, 7: 784-788
30. Rice-Evans C A, Miller N J, Paganga G (1997) Antioxidant properties of phenolic compounds, Trends Plant Sci, 2: 152–159
31. Robards K, Prenzler P D, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits, Food Chem, 66: 401–436
32. Schittler E J (1973) Introduction to Vinca alkaloids. In W Taylor (ed.), The Vinca alkaloids, New York: Inc, pp. 1-34
33. Schlesier K, Harwat M, Böhm V, Bitsch R (2002) Assessment of antioxidant activity by using different in vitro methods, Free Radical Res, 36: 177–187
34. Singleton V B, Rossi J A (1965) Colorimetry of total phenolic with phosphomolybdic-phosphotungstic acid reagents, Am J Enol Vitic, 16: 144-158
35. Snyder L R, Kirkland J J, Dolan J W (2009) Introduction to Modern Liquid Chromatography, John Wiley & Sons, New York
36. Sakushima A, Nishibe S (1988) Mass spectrometry in the structural determination of flavonol triglycosides from Vinca majör, Phytochem, 27: 915-919
37. Stearn W T (1978) Davis P H, ed. Flora of Turkey and the East Aegan Islands 6, Edinburgh, University Press, pp. 161-163
38. Tatsuzawa F (2015) Differences in the floral anthocyanin content of violet–blue flowers of Vinca minor L. and V. major L. (Apocynaceae), Phytochem Lett 13: 365–369