Artvin’de doğal yetişen Arbutus andrachne L.’nin meyve ve çiçeklerinin toplam fenolik madde içeriği ve antioksidan aktivitesinin belirlenmesi

Öz

Arbutus andrachne L. Akdeniz ve Ege bölgesinde doğal olarak bulunurken, Karadeniz bölgesinde Akdeniz enklav’ı olarak yayılır. Bu çalışmada Artvin’den alınan A. andrachne örneklerinin meyve ve çiçeklerinin antioksidan aktivite, toplam fenolik ve flavonoid madde içerikleri belirlendi. Antioksidan aktivite, DPPH radikal temizleme aktivite ve FRAP yöntemleri kullanılarak belirlenmiştir. Toplam fenolik madde miktarı Folin-Ciocalteu yöntemi, toplam flavonoid miktarı alüminyum klorür yöntemi kullanılarak tespit edilmiştir. Sonuçlara göre, A. andrachne’nin çiçekleri meyvelerinden daha yüksek antioksidan aktiviteye (104.81±4.75 μmol FeSO₄.7H₂O/g kuru madde) fenolik (43.57±2.85 mg GA/g kuru madde) ve flavonoid (114.28±1.49 mg Que/g dry sample) içeriğe sahiptir. A. andrachne’nin çiçek ve meyvelerinin iyi bir fenol ve antioksidan kaynağı olduğu sonucuna varılmıştır.

Anahtar Kelimeler

• Antioksidan,flavonoid,fenolik,Arbutus andrachne,Artvin.

Determining total phenolic content and antioxidant activity in fruits and flowers of naturally grown Arbutus andrachne L. in Artvin

Öz

Arbutus andrachne L. is widespread as Mediterranean enclave in the Black Sea Region, while it is found naturally in the Mediterranean and Aegean Region of Turkey. In this study, antioxidant activity, total phenolic and total flavonoid contents were determined in the fruits and flowers of A. andrachne samples obtained from Artvin. The antioxidant activity was analyzed using the DPPH radical scavenging activity and FRAP methods. In addition, while the total phenolic content was determined using the Folin-Ciocalteu assay, the aluminum chloride assay was used to measure the total flavonoid content. According to the results, the flowers of A. andrachne have higher contents in respect to antioxidant activity (104.81±4.75 μmol FeSO₄.7H₂O/g dry sample), phenolic (43.57±2.85 mg GA/g dry sample) and flavonoid (114.28±1.49 mg Que/g dry sample) than its fruits. It was concluded that the flowers and fruits of A. andrachne are good source of phenolic and antioxidant.

Anahtar Kelimeler

• Antioxidant,flavonoid,phenolic,Arbutus andrachne,Artvin.

Kaynakça

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