İki farklı tip tahribatsız portatif klorofil metre kullanarak Artvin’de yayılış gösteren sapsız meşede (Quercus, Fagaceae) klorofil tahmini

Öz

Bu çalışmada, SPAD-502Plus ve CCM-300 adlı optik klorofil içerik ölçer cihazlarından elde edilen klorofil içerik değerleri ile %80 aseton ile klorofil ekstraksiyon metodundan elde edilen klorofil içerik değerleri arasındaki ilişkinin saptanması amaçlanmıştır. Bu çalışma, sapsız meşenin, Quercus petraea (Mattuschka) Liebl. subsp. iberica (Steven ex Bieb.) Krassiln. alt türü üzerinde gerçekleştirilmiştir. Rakımı 889 m olan önceden belirlenmiş bir alandan benzer özelliklere sahip 11 ağaç seçilmiş, sağlıklı toplam 44 yaprak üzerinde ölçümler gerçekleştirilmiştir. Yapraklara ait SPAD, CFR (klorofil floresans) değerleri ve %80 aseton ile klorofil ekstraksiyonu devamında yapılan spektrofotometrik okumalar sonucunda elde edilen bulgular ayrı ayrı değerlendirilmiştir. Ölçümler sonucunda, her üç ölçüm tipi (SPAD, CFR ve %80 aseton metodu) sonuçlarının kuvvetli ilişki sergilediği tespit edilmiştir. Elde edilen SPAD ve CFR değerlerinin %80 aseton ile ekstraksiyon metodundan elde edilen verilere dönüştürülmesi amacıyla regresyon analizleri ile çeşitli eşitlikler oluşturulmuştur. Bu eşitlikler kullanılarak, SPAD ve CFR değerlerinin, kimyasal metodla tespit edilmiş klorofil içerik değerlerine dönüşümü mümkün olabilmektedir. Bu çalışmada, bu meşe türü için, birim alan başına yaprak toplam klorofil konsantrasyonu, SPAD (R²=0.9367) ve CFR (R² = 0.8702) okumaları arasındaki ilişkinin, taze ağırlık başına yaprak toplam klorofil konsantrasyonuna göre (SPAD (R² = 0.8264) ve CFR (R² = 0.7847)) daha yüksek olduğu tespit edilmiştir. Bu sonuçlara göre, bu optik cihazları kullanarak, tercihen birim alan başına (mg cm^-2), yaprak toplam klorofil içeriği çok kısa sürede ve kolaylıkla yapraklara zarar vermeden belirlenebilir.

Anahtar Kelimeler

• Aseton,CCM-300,Klorofil floresans,Sapsız meşe,SPAD-502Plus

Estimation of chlorophyll in sessile oak (Quercus, Fagaceae) naturally distributed in Artvin province using two different types of non-destructive portable chlorophyll meter

Öz

In this study, it was aimed to determine the relationship between the chlorophyll content values obtained from SPAD-502Plus and CCM-300 optical chlorophyll content meters and the chlorophyll content values obtained from the chlorophyll extraction method using 80% acetone. This study was carried out on a subspecies of the sessile oak (Quercus petraea (Mattuschka) Liebl. subsp. iberica (Steven ex Bieb.) Krassiln.). 11 trees with similar characteristics were selected from a predefined area with an altitude of 889 m and measurements were made on a total of 44 healthy leaves. The leaf SPAD values, CFR (chlorophyll fluorescence) values and the results obtained from the spectrophotometric readings of the chlorophyll extraction with 80% acetone were evaluated separately. As a result of the measurements, it was determined that the results of all three measurement types (SPAD, CFR and 80% acetone method) showed a strong correlation. Various equations were formed by regression analyses in order to convert the obtained SPAD and CFR values to the values obtained from the 80% acetone method. Using these equations, it is possible to convert SPAD and CFR values into chlorophyll content values determined by chemical method. In this study, for this type of oak, the relationship between the leaf total chlorophyll concentration per unit area and SPAD (R² = 0.9367) and CFR (R² = 0.8702)  readings was found higher than the leaf total chlorophyll concentration per fresh weight (SPAD (R² = 0.8264) and CFR (R² = 0.7847)). According to these results, by using these optical devices, preferably per unit area (mg cm^-2), the leaf total chlorophyll content can be determined in a very short time and easily without damaging the leaves.    

Anahtar Kelimeler

• Acetone,CCM-300,Chlorophyll fluorescence,Sessile oak,SPAD-502Plus

Kaynakça

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