In vitro antimicrobial, antioxidant activity and chemical characterization of Hypericum perforatum L. essentıal oil

Öz

In vitro antimicrobial and antioxidant activities and chemical composition of extracts prepared using two different solvents, hexane, ethanol, of commercially available essential oil sample isolated from Hypericum perforatum L. were determined. St. John's Wort essential oil was examined for various bacteria and fungi by using disc diffusion method to test its antimicrobial effectiveness. The chemical content of H. perforatum essential oil was determined by GC/MS method. It was concluded that hexane extract is more effective than ethanol extract against all bacterial and fungal strains. St. John's Wort oil had higher antimicrobial effects against fungi than bacteria. However, compared to standard antibiotics, activity is lower in the case of both ethanol and hexane extracts. The antioxidant activity of the 2% essential oil sample was also evaluated using 3 different methods and it was concluded that the oil sample's effectiveness of sweeping the DPPH radical was more effective than the other methods tested. With the chemical characterization as a result of GC/MS analysis 60 different compounds were identified including mainly carvacrol (4.78%), decane (8.15%), nonane (7.63%), p-cymene (9.04%), limonene (15.04%), α,p- dimethylstyrene (11.80%), camphor (4.21%), Isoborneol (%7.77) according to their highest area (%).

Anahtar Kelimeler

• Hypericum perforatum
• Antimicrobial
• Antioxidant

Proje Numarası

Bu araştırma, Ordu Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Dairesi tarafından TF-1635 numaralı projeyle desteklenmiştir.

Hyperıcum perforatum L. esansiyel yağının in vitro antimikrobiyal, antioksidan aktivite ve kimyasal karakterizasyonu

Öz

Ticari olarak temin edilen Hypericum perforatum L.’den izole edilmiş olan esansiyel yağ örneğinin hekzan ve etanol olmak üzere iki farklı çözgen kullanılarak hazırlanan ekstraktlarının in vitro antimikrobiyal ve antioksidan aktiviteleri ve kimyasal kompozisyonu belirlendi. Sarı kantaron esansiyel yağı antimikrobiyal etkinliğinin test edilebilmesi amacıyla disk difüzyon yöntemi kullanılarak bazı bakteri ve mantar türlerine karşı incelendi. H. perforatum esansiyel yağının kimyasal içeriği ise GC/MS yöntemi ile belirlendi. Tüm bakteri ve mantar suşlarına karşı hekzan özütünün etanol ekstraktına nazaran daha etkili olduğunu sonucuna varıldı. Sarı kantaron yağı funguslara karşı bakterilerden daha yüksek antimikrobiyal etki gösterdi. Ancak standart antibiyotiklerle karşılaştırıldığında hem etanol hem de hekzan ekstraktları durumunda aktivite daha düşüktür. esansiyel yağ %2 oranındaki numunesinin antioksidan aktivitesi de 3 farklı yöntem kullanılarak değerlendirilmiş ve örneğinin DPPH radikalini süpürme etkinliğinin test edilen diğer metotlara göre daha etkili olduğu sonucuna ulaşıldı. Yapılan kimyasal karakterizasyon çalışmalarıyla GC/MS analizleri sonucunda alan değeri (%) en yüksek olan bileşikler başlıca %4.78 karvakrol, %8.15 dekan, %7.63 nonan, %9.04 p-simen, %15.04 limonen, %11.80 alpha-para-dimetilstiren, %4.21 kamfor ve %7.77 isoborneol olmak üzere 60 farklı bileşik tanımlandı.

Anahtar Kelimeler

• antimikrobiyoloji
• antioxsidant
• Hypericum perforatum

Destekleyen Kurum

Ordu Üniversitesi BAP Birimi

Proje Numarası

Bu araştırma, Ordu Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Dairesi tarafından TF-1635 numaralı projeyle desteklenmiştir.

Teşekkür

Ordu Üniversitesi'ne ve Ordu Üniversitesi Merkez Araştırma Laboratuvarı'na maddi destekleri için teşekkür ederiz.

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