Effect of different plant materials on vegetative development of Lentinus edodes (Shiitake) edible mushroom

Öz

Lentinus edodes micelles (LEM), an edible cultivated mushroom, were investigated. In accordance with the purpose of the study; Maize Flour (MF), Rice Flour (RF) and Wheat Flour (WF) with 20%, 15%, 10% and 5% concentrations, MF, RF, WF, mixture and malt extract were used. The effect of different media on mycelial Development Time (MDT) has been shown. Mulberry Sawdust (MS), Poplar Sawdust (PS), Oak Sawdust (OS) and a mixture of these materials were used as ligno-cellulosic material in the seed mycelium and compost medium. Rice Bran (RB) and Wheat Bran (WB) were used as nitrogen source (22% bran:78% sawdust). The data obtained with the SPSS 17.0 statistical program were evaluated statistically. It was determined in the shortest 5% RF medium with 12.4 days of MGS, and the longest in Petri dishes with 20% WF with 17.4 days of MGS. In the production of seed mycelium; It was observed in the shortest mixture with MGT of 19.2 days, and the longest MS with MGS of 22.8 days. For the culture medium, 18 days of MGT and the shortest MS were seen in the medium with RB, and the longest with MGT of 36.8 days was seen in the medium without additives. In this study, the most suitable medium for the vegetative growth of Lentinus edodes edible fungus in the laboratory environment, 5% RF agar medium; It was concluded that the mixture medium for the production of seed mycelium (spawn) and the culture medium containing 78% MS and 22% RB for compost production are the most suitable media.

Anahtar Kelimeler

• Lentinus edodes
• Flour
• Sawdust
• Bran
• Shiitake

Lentinus edodes (Shiitake) yenilebilir mantarının vejetatif gelişmesi üzerine farklı bitkisel materyallerin etkisi

Öz

Mevcut çalışma ile yenilebilir bir kültür mantarı olan Lentinus edodes misellerinin (LEM) vejetatif gelişmesi üzerine farklı ligno-selülozik maddelerin etkileri incelenmiştir. Bu amaçla; Mısır Unu (MU), Pirinç Unu (PU) ve Buğday Ununun (BU), %20, %15, %10 ve %5’lik konsantrasyonları ile MU, PU, BU karışımı ve malt ekstrakt kullanıldı. Misel Gelişim Süresi (MGS) üzerine farklı besi yerlerinin etkisi gösterilmiştir. Tohumluk misel ve kompost ortamındaki ligno-selülozik madde olarak, Dut Talaşı (DT), Kavak Talaşı (KT), Meşe Talaşı (MT) ve bu materyallerin karışımı kullanıldı. Pirinç Kepeği (PK) ve Buğday Kepeği (BK) azot kaynağı olarak kullanıldı (%22 kepek:%78 talaş). SPSS 17.0 istatistik programı ile elde edilen veriler değerlendirildi. 12.4 günlük MGS ile en kısa %5’lik PU ortamında, 17.4 günlük MGS ile en uzun %20’lik BU’lu petrilerde belirlendi. Tohumluk misel eldesinde; 19.2 günlük MGS ile en kısa karışımda, 22.8 günlük MGS ile en uzun DT’de gözlendi. Kültür ortamı için 18 günlük MGS ile en kısa DT’nin PK’li ortamında, 36.8 günlük MGS ile en uzun KT’nin katkı maddesiz ortamında görüldü. Bu çalışma ile Lentinus edodes yenilebilir mantarının laboratuvar ortamındaki vejetatif gelişimi için en uygun besi yerinin, %5’lik PU’lu agar ortamının; tohumluk misel (spawn) eldesi için karışım ortamının ve kompost üretiminde ise %78 DT ile %22 PK içeren kültür ortamının en uygun ortamlar olduğu sonucuna varılmıştır.

Anahtar Kelimeler

• Lentinus edodes
• Un
• Talaş
• Kepek
• Shiitake

Kaynakça

1. Alagbaoso CA & Mizuno M (2021) Polysaccharides from Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Suppress pMLKL-Mediated Necroptotic Cell Death and Colitis in Mice. International Journal of Medicinal Mushrooms, 23
2. Alves de Lima PL, Delmanto RD, Sugui MM, Eira AF, Salvadori DMF, Speit G, Ribeiro LR (2001) Lentinula edodes (Berk.) Pegler (Shiitake) modulates genotoxic and mutagenic effects induced by alkylating agents in vivo. Mutation Research. 496(2001):23-32
3. Boztok K, Erkip N (2002) Meşe Mantarının (Lentinula edodes) ağaç kütükleri üzerinde yetiştiriciliği. Ege Üniv. Ziraat Fak. Dergisi, 39(1):149-155
4. aBruhn JN, Mihail JD (2009) Forest farming of Shiitake mushrooms: Aspects of forced fruiting. Bioresource Technology. 100(2009):5973-5978
5. bBruhn JN, Mihail JD, Pickens JB (2009) Forest farming of Shiitake mushrooms: An integrated evaluation of management practices. Bioresource Technology. 100(2009):6472-6480
6. Chen AW (2021) Cultivation of Lentinula edodes on synthetic logs. Erişim: [https://www.mushroomcompany.com/resources/shiitake/shiitake.pdf]. Erişim Tarihi:17.06.2021
7. Engel K, Golly I, Heinonen M, Lagiou P, Marchelli R, Moseley B, Neuhauser-Berthold M, Pöting A, Salminen S, Loveren HV, Verhagen H (2010) Scientific opinion on the safety of “Lentinus edodes extract” (Lentinex) as a novel food ingredient. EFSA journal. 8(7):1685
8. Enman J, Rova U, Berglund KA (2007) Quantification of the bioactive compound Eritadenine in selected strains of Shiitake mushroom (Lentinus edodes). J. Agric. Food Chem., 55 (4):1177-1180

9. Hatvani N (2001) Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. International Journal of Antimicrobial Agents, 17 (2001):71-74
10. Hatvani N, Mecs I (2001) Production of laccase and manganase peroxidase by Lentinus edodes on malt-containing by-product of the brewing process. Process Biochemistry. 37(2001):491-496
11. Hatvani N, Mecs I (2002) Effect of the nutrient composition on dye decolorisation and extracellular enzyme production by Lentinus edodes on solid medium. Enzyme and Microbial Technology. 30(2002):381-386
12. Hilal A & Yıldız A (2019). Pleurotus Sajor-Caju (Fr) Singer’in Yetiştiriciliği ve Verimi Üzerine Araştırmalar. Journal of the Institute of Science and Technology, 9(2), 717-725.
13. Huang Q, Qian X, Jiang T, Zheng X (2019) Effect of chitosan and guar gum based composite edible coating on quality of mushroom (Lentinus edodes) during postharvest storage Sci. Hortic., 253 (2019), pp. 382-389, 10.1016/j.scienta.2019.04.062
14. Hsiung DT, Marsit CJ, Houseman EA, Eddy K, Furniss CS, McClean MD, & Kelsey KT (2007) Global DNA methylation level in whole blood as a biomarker in head and neck squamous cell carcinoma. Cancer Epidemiology and Prevention Biomarkers, 16(1), 108-114
15. Leatham GF (1982) Cultivation of shiitake, the Japanese forest mushroom, on logs: a potential industry fort he United States. Forest Products Journal, 32(8):29-35
16. Lee S, Bea H, Kim N, Hwang S (2008) Optimization of growth conditions of Lentinus edodes Mycelium on corn processing waste using response surface analysis. Journal of Bioscience and Bioengineering. 105(2):161-163
17. Mizuno T (1995) Shiitake, Lentinus edodes: functional properties for medicinal and food purposes. Food Review İnternational. 1995(11):7-21
18. Morales D, Rutckeviski R, Villalva M, Abreu H, Soler-Rivas C, Santoyo S, Iacomini M, Smiderle FR (2020) Isolation and comparison of alpha- and beta-d-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities Carbohydr Polym, 229 (2020), Article 115521, 10.1016/j.carbpol.2019.115521
19. Özçelik E, & Pekşen A (2007) Hazelnut husk as a substrate for the cultivation of shiitake mushroom (Lentinula edodes). Bioresource technology, 98(14), 2652-2658
20. Rasmy GE, Botros WA, Kabeil SS, Daba AS (2010) Preparation of glucan from Lentinula edodes edible mushroom and elucidation of its medicinal value. Australian Journal of Basic and Applied Sciences. 4(11):5717-5726
21. Royse DJ (1985) Effect of spawn run time and substrat nutrition on yield and size of the Shiitake mushroom. Mycologia. 77(5):756-762
22. Royse DJ (2011) On natural and synthetic logs. Erişim [http://w.w.w.pubs.cas.psu.edu/Freepubs/pdfs/x10083.pdf.]. Erişim tarihi: 20.04.2011
23. Shen J, Tanida M, Fujisaki Y, Horii Y, Hashimoto K, Nagai K (2009) Effect of the culture extract of Lentinus edodes mycelia on splenic sympathetic activity and cancer cell proliferation. Autonomic Neuroscience: Basic And Clinical, 145 (2009):50-54
24. Shimada S, Komamura K, Kumagai H, Sakurai H (2004) İnhibitory activity of Shiitake flavor against platelet aggregation. Biofactors. 22(1-4):177-179
25. Spim SRV, Pistila AMH, Pickler TB, Silva MT & Grotto D (2021) Effects of Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Bars on Lipid and Antioxidant Profiles in Individuals with Borderline High Cholesterol: A Double-Blind Randomized Clinical Trial. International Journal of Medicinal Mushrooms, 23(7)
26. Stamets P (1993, 2000) Growing gourmet and medicinal mushroom. ten speed pres, New 3rd edition Berkeley, California, pp:259-276
27. Sugui MM, Alves de Lima PL, Delmanto, RD, Eira AF, Salvadori DMF, Ribeiro LR (2003) Antimutagenic effect of Lentinula edodes (Berk.) Pegler mushroom and possible variation amond lineages. Food and Chemical Toxicology. 41(2003):555-560.
28. Wasser SP (2011) Shiitake (Lentinus edodes) Erişim: [http://www.alohamedicinals.com/shiitake.pdf]. Erişim Tarihi:09.05.2011.
29. Watanabe K (2001) Current cultivation techniques of shiitake on sawdust media in Japan. 15th North American mushroom conference, Şubat 2001, Las Vegas, U.S.A.
30. Worrall JJ, Yang CS (1992) Shiitake and Oyster mushroom production on apple pomace and sawdust. Hortscience. 27(10):1131-1133
31. Wu CH, Wu CC, Ho YS (2007) Antitumor activity of combination treatment of Lentinus edodes mycelium extracts with 5-fluorouracil against human colon cancer cells xenografted in nude mice. Journal of Cancer Molecules. 31 (1) 15-22
32. Yang P, Liang M, Zhang Y, Shen B (2010) Clinical application of a combination therapy of lentinan, multi-electrode RFA and TACE in HCC. Advences in Therapy. 25(8):787-794
33. Zhang M, Cui SW, Cheung PCK, Wang Q (2007) Antitumor polysaccharides from mushrooms: A review on their isolation process, structural characteristics and antitumor activity. Trends In Food Science&Technology. 18(2007) 4-19
34. Zheng Z, Shetty K (2000) Solid state production of polygalacturonase by Lentinus edodes using fruit processing wastes. Process Biochemistry. 35(2000):825-830