Investigation of the effects of acetone o-(4 chlorophenylsulfonyl) oxime pre-application on biochemical parameters of maize seedlings under salt stress

Öz

The main purpose of the current study is to investigate if the pretreatment of acetone O-(4 chlorophenylsulfonyl) oxime (AO) molecule on maize seedlings under salt stress has mitigating effects on the adverse effects of stress or not. The following experimental setup was established: 18 hours distilled water control (K), 6 hours AO+12 hours distilled water (AO), 6 hours distilled water+then 12 hours 100 mM NaCl (TS) and 6 hours AO+then 12 hours 100 mM NaCl (AO+TS). According to the findings; While there was no difference between the control application and the AO application in terms of relative water content (RSI), it was determined that there was a significant decrease in TS and a significant increase in AO+TS compared to the control. While the chlorophyll content decreased in TS application compared to AO and control, the content increased significantly in AO+TS application compared to TS. While the highest carotenoid content was observed in the TS application, the lowest content was determined in AO+TS. While a significant decrease was observed in MDA and H2O2 contents in AO application compared to the control, a significant increase in TS compared to the control and a significant decrease in AO+TS compared to TS were determined. It was determined that guaiacol peroxidase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes significantly reduced MDA and H2O2 content by regulating their activities with AO pre-application. While a significant increase was observed in proline content with AO application compared to control, it was determined that AO+TS caused a significant decrease in content compared to TS application. It was observed that AO application caused significant changes in the phenolic substance content. In the light of the findings, it is thought that it can be concluded that AO pre-application to maize seedlings under salt stress can prevent the formation of radicals that have the potential to inhibit the general functioning of metabolism.

Anahtar Kelimeler

• Maize
• Salt stress
• Acetone O - (4-chlorophenylsulfonyl) Oxime (AO)
• Antioxidant system

Tuz stresi altındaki mısır fidelerine aseton o-(4 klorofenilsülfonil) oksim ön uygulamasının biyokimyasal parametreler üzerine etkilerinin araştırılması

Öz

Mevcut çalışmanın temel amacı, tuz stresi altındaki mısır fidelerine aseton O-(4 klorofenilsülfonil) oksim (AO) molekülünün ön muamelesinin stresin olumsuz etkilerini hafifletici etkilerinin olup olmadığının araştırılmasıdır. Bunun için; 18 saat distile su kontrol (K), 6 saat AO+12 saat distile su (AO), 6 saat distile su+12 saat 100 mM NaCl (TS) ve 6 saat AO+12 saat 100 mM NaCl (AO+TS) deney düzeneği kurulmuştur. Elde edilen bulgulara göre; kontrol uygulaması ile AO uygulaması arasında nispi su içeriği (NSİ) açısından bir fark saptanamazken, TS’de ciddi bir düşüş AO+TS’de ise kontrole göre önemli bir artış olduğu belirlendi. Klorofil içeriği TS uygulamasında AO ve kontrole göre azalırken, AO+TS uygulamasında içerik TS’ye göre önemli bir artış gösterdi. En yüksek karotenoid içeriği TS uygulamasında görülürken, en düşük içerik AO+TS’de belirlendi. MDA ve H2O2 içeriklerinde AO uygulamasında kontrole göre önemli bir azalış gözlenirken, TS’de kontrole göre ciddi bir artış AO+TS’de ise TS ile kıyaslandığında önemli bir azalış belirlendi. Guaiacol peroksidaz, katalaz, askorbat peroksidaz ve süperoksit dismutaz enzimleri AO ön uygulaması ile aktivitelerini düzenleyerek MDA ve H2O2 içeriğini önemli ölçüde azalttığı belirlendi. AO uygulaması ile prolin içeriğinde kontrole göre önemli bir artış gözlenirken, AO+TS’nin TS uygulamasına göre içerikte önemli bir azalışa neden olduğu belirlendi. AO uygulamasının fenolik madde içeriği üzerinde önemli değişikliklere neden olduğu gözlendi. Elde edilen bulgular ışığında, tuz stresi altındaki mısır fidelerine AO ön uygulamasının metabolizmanın genel işleyişini engelleme potansiyeline sahip radikallerin oluşumunu engelleyebileceğini düşündürmektedir.

Anahtar Kelimeler

• Mısır
• Tuz stresi
• Aseton O-(4-klorofenilsülfonil)Oksim (AO)
• Antioksidan sistem

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