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TÜRK VE SURİYELİ BİREYLERİN KONJONKTİVAL FLORALARININ KARŞILAŞTIRILMASI

Yıl 2025, Cilt: 5 Sayı: 1, 20 - 25, 13.01.2025

Öz

Amaç: Mülteci sorunu uzun yıllardır tüm dünyada devam eden ve giderek daha da büyük boyutlara ulaşan bir sorundur. Türkiye’ye gelen Suriyeli mültecileri misafir eden ülkelerin başında gelmektedir. Bu çalışmamızda kliniğimize başvuran Türk bireyler ile Suriyeli mültecilerin konjonktival floralarının değerlendirilmesi ve sonuçların karşılaştırması amaçlandı.
Materyal ve Metod: Çalışmaya 55 ‘i Türk, 41’i Suriyeli toplam 96 hasta dahil edildi. Hastaların alt göz kapağı konjonktivasından steril eküvyon ile sürüntü örneği alındı. Alınan örneklerin mikrobiyoloji laboratuvarında Eosin Metilen Blue (EMB) agar, Sabouraud dekstroz agar, çukulatamsı agar ve %5 koyun kanlı agar besiyerlerine ekimleri yapıldı.
Bulgular: Suriyeli mültecilerde konjonktival kültür pozitifliği %53.7 iken Türk bireylerde %.52.7 oranında pozitif kültür saptandı (p=0.92). Coagulase Negative Staphylococcus (CNS) Türklerde (%32.7) ve Suriyeli mültecilerde (%46.3) en sık izole edilen mikroorganizma olarak bulundu (p=0.19). Türklerde CNS 18’inde (%32.7), ikinci en sık izole edilen Streptococcus spp. 10’unda (%18.2) , Staphylococcus aureus 7’sinde (%12.7), Corynebacterium spp. 6’sında (%10.9), Bacillus spp. 7’sinde (%12.7), Micrococcus spp 1’inde.( %1.8), Neisseria spp. 1’inde (%1.8) izole edilmiştir. Suriyeli mültecilerin konjonktival kültürlerinde ise CNS 19’unda (%46.3), Corynebacterium spp. 7’sinde (%17.1), Streptococcus spp. 3’ünde( %7.3), Staphylococcus aureus 2’sinde (%4.9), Bacillus spp. 5’inde (%12.2), Micrococcus spp 1’inde (%1) izole edilmiştir.
Sonuç: Türklerde ve Suriyeli mültecilerde konjonktival üreme benzer ve en sık izole edilen bakteri CNS bulunmuştur. İzole edilen bakteri türleri iki grupta benzerdir ve bu bakterilerin görülme sıklıkları açısından da istatiksel olarak anlamlı fark görülmemiştir.

Kaynakça

  • 1. Jafferji SS, Ilako DR, Kollmann KHM, Kariuki MM, Schaller UC. The antibiotic sensitivity pattern of coagulase negative staphylococci; a major ocular normal flora. East Afr J Ophthalmol. PhD Thesis.2007;27-33.
  • 2. Richard FB . Jeffrey BR. Anaerobic endophthalmitis caused by Propionibacterium acnes. Am J of Oph. 1986; 101(1):114-116
  • 3. James BK, Kenneth DT. Anterior chamber aspirate cultures after uncomplicated cataract surgery. Am J of Oph. 1991;112(3):278-282.
  • 4. Aril'S . Leon DS . Mark AM . Anterior chamber contamination after uncomplicated phacoemulsification and intraocular lens implantation. Am J of Oph. 1995; 120(2):143-150.
  • 5. Therese KL&Madhavan HN. Microbiological Procedures For Diagnosis of ocular infections, L & T Microbiology Research Centre, Vision Research Foundation 18, College Road, Chennai. 2004;600 006.
  • 6. Birinci H, Birinci A, Şahin M, ve Öge F, Öge D. Konjonktival floranın insülin kulanan diabetik hastalar ile kontrollerde karşılaştırılması. T Oft Gaz. 1998(28);28:144-146.
  • 7. UNHCR - Global Trends Report. https://www.unhcr.org/globaltrends2019/
  • 8. Afet Raporu/Suriye. https://www.afad.gov.tr/tr
  • 9. Orhan O, Gündoğar SS. Suriyeli Sığınmacıların Türkiye’ye Etkileri. ORSAM Raporu 2015.
  • 10. Korkmaz AÇ. Sığınmacıların Sağlık ve Hemşirelik Hizmetlerine Yarattığı Sorunlar. Sağlık ve Hemşirelik Yönetimi Dergisi 2014;1:37-42
  • 11. Sherwood DR, Rich WJ, Jacob JS, Hart RJ, Fairchild YL. Bacterial contamination of intraocular and extraocular fluids during extracapsular cataract extraction. Eye (Lond). 1989;3(3):308–12.
  • 12. Piest KL, Kincaid MC, Tetz MR, Apple DJ, Roberts WA, Price FW, Jr, et al. Localized endophthalmitis: A newly described cause of the so-called toxic lens syndrome. J Cataract Refract Surg. 1987;13(5):498–510
  • 13.. Mino de Kaspar H, Koss MJ, He L, Blumenkranz MS, Ta CN. Antibiotic susceptibility of preoperative normal conjunctival bacteria. Am J Ophthalmol. 2005;139:730-3.
  • 14. Spiegel P, Khalifa A, Mateen FJ. Cancer in refugees in Jordan and Syria between 2009 and 2012: challenges and the way forward in humanitarian emergencies. Lancet Oncol. 2014; 15(7): 290-7.
  • 15. Manav G, Bilgin L, Gezer A. Normal populasyonda konjonktival flora. T. Oft. Gaz. 1992;2:121-4.
  • 16. Korkmaz A. Suriyeli Sığınmacılardan Kaynaklanan Sorunlar ve Çözüm Önerileri. Akademik Hassasiyetler Dergisi, 2016;3(6):.83-116.
  • 17. Karadağ Çaman Ö., Bahar Özvarış Ş. Uluslararası Göç ve Kadın Sağlığı. Sağlık ve Toplum Dergisi, 2010;20(4):3-14.
  • 18. Smith CH. Bacteriology of the healthy conjunctiva. Br J Ophthalmol. 1955;34(9):580-585.
  • 19. Saleem Q, Arbab TM, Badvi JA. Aerobic flora of normal human conjunctival sac. Med Channel. 2009;15:65–7.
  • 20. Puttana ST, D'Souza C, Bhargava MK. Bacterial flora of the eye: A preoperative study. Proc All India Ophthalmol Soc. 1958;18:150–7.
  • 21. Saxena H, Goswami P. Bacterial and fungal flora of the normal eye. Indian J Ophthalmol. 1971;19(3):130–5.
  • 22. Hogan MJ, Zimmerman LE. An Atlas and Text Book of Ophthalmology. 2nd ed. Philadelphia: W.B. Saunders Co.; 1962. p.235–40.
  • 23. Liu J, Li J, Huo J, Xie H. Identification and quantitation of conjunctival aerobic acterial flora from healthy residents at different ages in Southwest China. Afr J Microbiol Res. 2011;5(3):192–7.
  • 24. Rajvanshi VS, Sahai VB, Mehrotra TN. Distribution and phage types of coagulase positive staphylococci isolated from different sources. Indian J Pathol Bacteriol. 1967;10(2):155–64.
  • 25. Park SH, Lim JA, Choi JS, Kim KA, Joo CK. The resistance patterns of normal ocular bacterial flora to 4 fluoroquinolone antibiotics. Cornea. 2009;28(1):68–72.
  • 26. Bilgehan H. Klinik Mikrobiyolojik Tanı. Fakülteler Kitapevi, Barış yayınları, 2009.
  • 27. Nakata K, Inoue Y, Harada J, Maeda N, Watanabe H, Tano Y, et al. A high incidence of staphylococcus aureus colonization in the external eyes of patients with atopic dermatitis. Ophthalmology. 2000; 107(12): 2167-71.
  • 28. Au YK, Jensen HG, Rowsey J, Reynolds M. Coagulase–negative staphylococci in conjunctivitis and blepharitis. Yan Ke Xue Bao=Eye Science .1993; 9(3): 129-35.
  • 29. Seal D, Ficker L, Ramakrishnan M, Wright P. Role of staphylococcal toxin production in blepharitis. Ophthalmology. 1990; 97(12): 1684-8.
  • 30. Tetz MR, Klein U, Volcker HE. Staphylococcus- associated blepharokeratoconjunctivitis. Clinical findings, pathogenesis and therapy. Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft.1997; 94(3): 186-90.
  • 31. Bowman RW, McCulley JP. Ocular bacteriology. In: Albert DM, Jakobiec FA, editors. Principles and Practice of Ophthalmology. Philadelphia: W.B. Saunders Company .1994.p. 816-834.
  • 32. Trindale RC, Bonfim ACR and Resende MA. Conjunctival microbial flora of clinically normal persons who work in a hospital environment. Brazilian Journal of Microbiology.2000;31(1):12-16.
  • 33. Önal A., Keklik B. Mülteci ve Sığınmacıların Sağlık Hizmetlerine Erişimde Yaşadığı Sorunlar: Isparta İlinde Bir Uygulama. Süleyman Demirel Üniversitesi Vizyoner Dergisi.2016;7(15):132-148.).
  • 34 Karataştan, N. Suriyeli mültecilerin sağlık hizmetlerine ulaşmada yaşadıkları zorluklar. 2017. Master's Thesis. Sağlık Bilimleri Enstitüsü.

Comparison of Conjunctival Flora of Turkish and Syrian Individuals

Yıl 2025, Cilt: 5 Sayı: 1, 20 - 25, 13.01.2025

Öz

Aim: The refugee problem is a problem that has been going on all over the world for many years and has reached increasingly larger dimensions. Turkey is one of the leading countries hosting Syrian refugees. In this study, we aimed to evaluate the conjunctival flora of Syrian refugees and Turkish individuals admitted to our clinic and to compare the results.
Materials and Methods: A total of 96 patients, 55 Turkish and 41 Syrian, were included in the study. A swab was taken from the conjunctiva of the lower eyelid of the patients with a sterile swab. The samples were cultured on Eosin Methylene Blue (EMB) agar, Sabouraud dextrose agar, chocolate agar and 5% sheep blood agar media in the microbiology laboratory.
Results: Conjunctival culture positivity was 53.7% in Syrian refugees and 52.7% in Turkish individuals (p=0.92). Coagulase Negative Staphylococcus (CNS) was the most frequently isolated microorganism in Turks (32.7%) and Syrian refugees (46.3%) (p=0.19). CNS was isolated in 18 (32.7%) Turks, Streptococcus spp. was isolated in 10 (18.2%), Staphylococcus aureus in 7 (12.7), Corynebacterium spp. in 6 (10.9%), Bacillus spp. in 7 (12.7%), Micrococcus spp. in 1 (1.8%), Neisseria spp. in 1 (1.8%). In conjunctival cultures of Syrian refugees, CNS was isolated in 19 (46.3%), Corynebacterium spp. in 7 (17.1%), Streptococcus spp. in 3 (7.3%), Staphylococcus aureus in 2 (4.9%), Bacillus spp. in 5 (12.2%), Micrococcus spp. in 1 (1%).
Conclusion: Conjunctival growth was similar in Turks and Syrian refugees and CNS was the most frequently isolated bacterium. The bacterial species isolated were similar in the two groups and there was no statistically significant difference in the frequency of these bacteria.

Kaynakça

  • 1. Jafferji SS, Ilako DR, Kollmann KHM, Kariuki MM, Schaller UC. The antibiotic sensitivity pattern of coagulase negative staphylococci; a major ocular normal flora. East Afr J Ophthalmol. PhD Thesis.2007;27-33.
  • 2. Richard FB . Jeffrey BR. Anaerobic endophthalmitis caused by Propionibacterium acnes. Am J of Oph. 1986; 101(1):114-116
  • 3. James BK, Kenneth DT. Anterior chamber aspirate cultures after uncomplicated cataract surgery. Am J of Oph. 1991;112(3):278-282.
  • 4. Aril'S . Leon DS . Mark AM . Anterior chamber contamination after uncomplicated phacoemulsification and intraocular lens implantation. Am J of Oph. 1995; 120(2):143-150.
  • 5. Therese KL&Madhavan HN. Microbiological Procedures For Diagnosis of ocular infections, L & T Microbiology Research Centre, Vision Research Foundation 18, College Road, Chennai. 2004;600 006.
  • 6. Birinci H, Birinci A, Şahin M, ve Öge F, Öge D. Konjonktival floranın insülin kulanan diabetik hastalar ile kontrollerde karşılaştırılması. T Oft Gaz. 1998(28);28:144-146.
  • 7. UNHCR - Global Trends Report. https://www.unhcr.org/globaltrends2019/
  • 8. Afet Raporu/Suriye. https://www.afad.gov.tr/tr
  • 9. Orhan O, Gündoğar SS. Suriyeli Sığınmacıların Türkiye’ye Etkileri. ORSAM Raporu 2015.
  • 10. Korkmaz AÇ. Sığınmacıların Sağlık ve Hemşirelik Hizmetlerine Yarattığı Sorunlar. Sağlık ve Hemşirelik Yönetimi Dergisi 2014;1:37-42
  • 11. Sherwood DR, Rich WJ, Jacob JS, Hart RJ, Fairchild YL. Bacterial contamination of intraocular and extraocular fluids during extracapsular cataract extraction. Eye (Lond). 1989;3(3):308–12.
  • 12. Piest KL, Kincaid MC, Tetz MR, Apple DJ, Roberts WA, Price FW, Jr, et al. Localized endophthalmitis: A newly described cause of the so-called toxic lens syndrome. J Cataract Refract Surg. 1987;13(5):498–510
  • 13.. Mino de Kaspar H, Koss MJ, He L, Blumenkranz MS, Ta CN. Antibiotic susceptibility of preoperative normal conjunctival bacteria. Am J Ophthalmol. 2005;139:730-3.
  • 14. Spiegel P, Khalifa A, Mateen FJ. Cancer in refugees in Jordan and Syria between 2009 and 2012: challenges and the way forward in humanitarian emergencies. Lancet Oncol. 2014; 15(7): 290-7.
  • 15. Manav G, Bilgin L, Gezer A. Normal populasyonda konjonktival flora. T. Oft. Gaz. 1992;2:121-4.
  • 16. Korkmaz A. Suriyeli Sığınmacılardan Kaynaklanan Sorunlar ve Çözüm Önerileri. Akademik Hassasiyetler Dergisi, 2016;3(6):.83-116.
  • 17. Karadağ Çaman Ö., Bahar Özvarış Ş. Uluslararası Göç ve Kadın Sağlığı. Sağlık ve Toplum Dergisi, 2010;20(4):3-14.
  • 18. Smith CH. Bacteriology of the healthy conjunctiva. Br J Ophthalmol. 1955;34(9):580-585.
  • 19. Saleem Q, Arbab TM, Badvi JA. Aerobic flora of normal human conjunctival sac. Med Channel. 2009;15:65–7.
  • 20. Puttana ST, D'Souza C, Bhargava MK. Bacterial flora of the eye: A preoperative study. Proc All India Ophthalmol Soc. 1958;18:150–7.
  • 21. Saxena H, Goswami P. Bacterial and fungal flora of the normal eye. Indian J Ophthalmol. 1971;19(3):130–5.
  • 22. Hogan MJ, Zimmerman LE. An Atlas and Text Book of Ophthalmology. 2nd ed. Philadelphia: W.B. Saunders Co.; 1962. p.235–40.
  • 23. Liu J, Li J, Huo J, Xie H. Identification and quantitation of conjunctival aerobic acterial flora from healthy residents at different ages in Southwest China. Afr J Microbiol Res. 2011;5(3):192–7.
  • 24. Rajvanshi VS, Sahai VB, Mehrotra TN. Distribution and phage types of coagulase positive staphylococci isolated from different sources. Indian J Pathol Bacteriol. 1967;10(2):155–64.
  • 25. Park SH, Lim JA, Choi JS, Kim KA, Joo CK. The resistance patterns of normal ocular bacterial flora to 4 fluoroquinolone antibiotics. Cornea. 2009;28(1):68–72.
  • 26. Bilgehan H. Klinik Mikrobiyolojik Tanı. Fakülteler Kitapevi, Barış yayınları, 2009.
  • 27. Nakata K, Inoue Y, Harada J, Maeda N, Watanabe H, Tano Y, et al. A high incidence of staphylococcus aureus colonization in the external eyes of patients with atopic dermatitis. Ophthalmology. 2000; 107(12): 2167-71.
  • 28. Au YK, Jensen HG, Rowsey J, Reynolds M. Coagulase–negative staphylococci in conjunctivitis and blepharitis. Yan Ke Xue Bao=Eye Science .1993; 9(3): 129-35.
  • 29. Seal D, Ficker L, Ramakrishnan M, Wright P. Role of staphylococcal toxin production in blepharitis. Ophthalmology. 1990; 97(12): 1684-8.
  • 30. Tetz MR, Klein U, Volcker HE. Staphylococcus- associated blepharokeratoconjunctivitis. Clinical findings, pathogenesis and therapy. Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft.1997; 94(3): 186-90.
  • 31. Bowman RW, McCulley JP. Ocular bacteriology. In: Albert DM, Jakobiec FA, editors. Principles and Practice of Ophthalmology. Philadelphia: W.B. Saunders Company .1994.p. 816-834.
  • 32. Trindale RC, Bonfim ACR and Resende MA. Conjunctival microbial flora of clinically normal persons who work in a hospital environment. Brazilian Journal of Microbiology.2000;31(1):12-16.
  • 33. Önal A., Keklik B. Mülteci ve Sığınmacıların Sağlık Hizmetlerine Erişimde Yaşadığı Sorunlar: Isparta İlinde Bir Uygulama. Süleyman Demirel Üniversitesi Vizyoner Dergisi.2016;7(15):132-148.).
  • 34 Karataştan, N. Suriyeli mültecilerin sağlık hizmetlerine ulaşmada yaşadıkları zorluklar. 2017. Master's Thesis. Sağlık Bilimleri Enstitüsü.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Mikrobiyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Zarife Ekici Gök 0000-0002-9250-9618

Ayten Gündüz 0000-0003-2031-9978

Yayımlanma Tarihi 13 Ocak 2025
Gönderilme Tarihi 2 Aralık 2024
Kabul Tarihi 31 Aralık 2024
Yayımlandığı Sayı Yıl 2025 Cilt: 5 Sayı: 1

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