IMMUNOHISTOCHEMICAL EXAMINATION OF NUCLEAR FACTOR KAPA B ACTIVATION IN INDIVIDUALS WITH AGGRESSIVE PERIODONTITIS: STEREOLOGIC STUDY

Öz

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Anahtar Kelimeler

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AGRESİF PERİODONTİTİSLİ BİREYLERDE NÜKLEER FAKTÖR KAPPA B AKTİVASYONUN İMMUNOHİSTOKİMYASAL OLARAK İNCELENMESİ: STEREOLOJİK ÇALIŞMA

Öz

Bu çalışmanın amacı; agresif periodontitisli bireylerin diş eti dokularında Nükleer Faktör kappa B (NFкB) proteinlerinden p50 ve p65 alt ünitelerinin çekirdek ve sitoplazmik ekspresyonunun ve NF-кB inhibitörü olan İnhibitör kappa B’nin (IкB) sitoplazmik ekspresyonunun immunohistokimyasal ve stereolojik olarak araştırılmasıdır. Çalışma popülasyonunu, Atatürk Üniversitesi Diş Hekimliği Fakültesine 2006-2007 yılları arasında değişik nedenlerle müracaat eden 30 birey (15’i agresif periodontitisli ve 15’i periodontal olarak sağlıklı) oluşturdu. Tüm bireylerden lokal anestezi altında dişlerin bukkal dişeti bölgesinden biyopsi ile 1–2 mm diş eti dokusu alındı. Alınan dişeti dokuları hemen % 10’luk nötral tamponlu formalin içerisine konuldu ve sonra immunohistokimyasal boyamaya gönderildi. Elde edilen kesitler elektron mikroskobu altında incelendi ve stereolojik yöntem kullanılarak boyanan hücre sayısal değerleri karşılaştırmalarında Tek Yönlü Varyans Analizi ve Çoklu karşılaştırmalı en küçük önemli fark (LSD) testi (p=0.05) kullanıldı

Anahtar Kelimeler

• Agresif periodontitis, nükleer faktör-kappa B, stereoloji

Kaynakça

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