THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS

Suleyman Emre Meşeli; Gonca Erköse Genç; Gizem Çolakoğlu; Suleyman Pelit; Esra Koç; Birsay Gümrü Tarçın

doi:10.17567/ataunidfd.566861

THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS

Öz

Abstract

Aim: CAim: Candida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.
andida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.

Materials and Methods: The 56 non-smoker, systemically healthy, maxillary removable denture wearer subjects included in this study and diagnosed as DRS(N=27) and healthy(N=29). Samples from the palatal mucosal surface of patients were collected by sterile swabs during 20 seconds. Cultivation and selective isolation of Candida species were performed on CHROMagar (CHROMagar^® Candida, CHROMagar, Paris, France) at 37ºC for 2 days. Isolated Candida species were identified with API ID 32 C (bioMérieux^®, France). Biofilm formation by Candida species were determined by a visual tube method.

Results: The prevalence of the yeasts in the healthy group was found to be 37.94%, while in the DRS group it was 100% (p<0.001). The isolation rate of C. albicans in patients with DRS significantly higher (p<0.001). Biofilm formation was observed in a total of 37 oral yeast isolates, 9 isolates from healthy denture wearers and 28 isolates from DRS patients. The number of C. albicans and C. glabrata isolates showing biofilm formation ability in the DRS group was significantly higher than the healthy group (p<0.05).

Conclusions: Within the limitations of this study, our results suggest that Candida species play a major role on the development of DRS. While C. albicans was the most isolated species in DRS, C. glabrata was as important as C. albicans on the pathogenesis of DRS.

keywords: Biofilms, Candida, denture stomatitis, mycology, oral pathology

HAREKETLİ PROTEZ KULLANAN HASTALARDA KANDİDA TÜRLERİNİN KOLONİZASYONUNUN VE BİYOFİLM OLUŞTURMA KAPASİTELERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Candida türleri, oral floranın doğal üyesi olmakla birlikte, belirli koşullar altında patolojik özellikler sergileyebilir. Bu çalışmanın amacı, protez stomatiti (PS) olan hastalardan ve protez kullanan sağlıklı bireylerden elde edilen Candida türlerinin dağılımını ve biyofilm üretime kapasitelerini karşılaştırmaktır

Gereç ve Yöntem: Sigara içmeyen, sistemik olarak sağlıklı, maksiller tam damak protez kullanan 56 kişi bu çalışmaya dahil edildi. Katılımcılar PS (N = 27) ve sağlıklı (N = 29) olmak üzere gruplandırıldı. Hastaların palatal mukoza yüzeyinde 20 saniye boyunca steril swablar gezdirilerek sürüntü örnekleri elde edildi. Candida türlerinin kültivasyon ve seçici izolasyonu 2 gün boyunca 37 ° C'de CHROMagar (CHROMagar® Candida, CHROMagar, Paris, Fransa) besiyerinde gerçekleştirildi. İzole edilen Candida türlerinin identifikasyonu API ID 32 C (bioMérieux®, Fransa) ile gerçekleştirildi. Candida türlerinin biyofilm oluşturma potansiyelleri görsel tüp yöntemi ile saptandı
Bulgular: Sağlıklı gruptaki mayaların prevalansı % 37.94 iken, PS grubunda % 100'dü (p <0.001). PS'li hastalarda C. albicans'ın izolasyon oranı anlamlı derecede daha yüksekti (p <0.001). Sağlıklı katılımcılardaki 9 PS katılımcılardaki 28 izolatta biyofilm oluşumu gözlendi. PS grubunda biyofilm oluşumunu gösteren C. albicans ve C. glabrata izolatlarının sayısı sağlıklı gruba göre anlamlı olarak yüksekti (p <0.05).

Sonuç: Bu çalışmanın sınırları dahilinde, sonuçlarımız Candida türlerinin PS'nin gelişiminde önemli bir rol oynadığını göstermektedir. C. albicans, PS'de en sık izole edilen tür olsa da C. glabrata da PS'nin patogenezinde C. albicans kadar önemlidir.

Anahtar Kelimeler: Biyofilm, Kandida, protez stomatitisi, mikoloji, oral patoloji

Anahtar Kelimeler

Biofilms,Candida,denture stomatitis,mycology

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Diş Hekimliği

Bölüm

Araştırma Makalesi

Yazarlar

Suleyman Emre Meşeli Bu kişi benim
0000-0002-8922-155X

Gonca Erköse Genç Bu kişi benim
0000-0002-8983-9675

Gizem Çolakoğlu Bu kişi benim
0000-0002-9471-9993

Suleyman Pelit Bu kişi benim
0000-0002-0028-4264

Esra Koç Bu kişi benim
0000-0003-0435-3607

Birsay Gümrü Tarçın
0000-0002-7734-4755

Yayımlanma Tarihi

16 Temmuz 2019

Gönderilme Tarihi

10 Ocak 2019

Kabul Tarihi

17 Mayıs 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 29 Sayı: 3

DOI

https://doi.org/10.17567/ataunidfd.566861

IZ

https://izlik.org/JA99BD35CF

Kaynak Göster

RIS / Bibtex

APA

Meşeli, S. E., Erköse Genç, G., Çolakoğlu, G., Pelit, S., Koç, E., & Gümrü Tarçın, B. (2019). THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 29(3), 387-393. https://doi.org/10.17567/ataunidfd.566861

AMA

1.Meşeli SE, Erköse Genç G, Çolakoğlu G, Pelit S, Koç E, Gümrü Tarçın B. THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Ata Diş Hek Fak Derg. 2019;29(3):387-393. doi:10.17567/ataunidfd.566861

Chicago

Meşeli, Suleyman Emre, Gonca Erköse Genç, Gizem Çolakoğlu, Suleyman Pelit, Esra Koç, ve Birsay Gümrü Tarçın. 2019. “THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29 (3): 387-93. https://doi.org/10.17567/ataunidfd.566861.

EndNote

Meşeli SE, Erköse Genç G, Çolakoğlu G, Pelit S, Koç E, Gümrü Tarçın B (01 Temmuz 2019) THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29 3 387–393.

IEEE

[1]S. E. Meşeli, G. Erköse Genç, G. Çolakoğlu, S. Pelit, E. Koç, ve B. Gümrü Tarçın, “THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS”, Ata Diş Hek Fak Derg, c. 29, sy 3, ss. 387–393, Tem. 2019, doi: 10.17567/ataunidfd.566861.

ISNAD

Meşeli, Suleyman Emre - Erköse Genç, Gonca - Çolakoğlu, Gizem - Pelit, Suleyman - Koç, Esra - Gümrü Tarçın, Birsay. “THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29/3 (01 Temmuz 2019): 387-393. https://doi.org/10.17567/ataunidfd.566861.

JAMA

1.Meşeli SE, Erköse Genç G, Çolakoğlu G, Pelit S, Koç E, Gümrü Tarçın B. THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Ata Diş Hek Fak Derg. 2019;29:387–393.

MLA

Meşeli, Suleyman Emre, vd. “THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 29, sy 3, Temmuz 2019, ss. 387-93, doi:10.17567/ataunidfd.566861.

Vancouver

1.Suleyman Emre Meşeli, Gonca Erköse Genç, Gizem Çolakoğlu, Suleyman Pelit, Esra Koç, Birsay Gümrü Tarçın. THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Ata Diş Hek Fak Derg. 01 Temmuz 2019;29(3):387-93. doi:10.17567/ataunidfd.566861