PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ

Serhat Karaca; Şirin Güner Onur; İlker Tinay; Süheyla Uyar Bozkurt; Ali – Orcid Number: Menteş

doi:10.17567/ataunidfd.783461

PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ

Öz

Amaç: Bu çalışmanın amacı prenatal dönemden itibaren değişik konsantrasyonlarda sistemik fluora (F) maruz kalan sıçanlarda; F’nin, böbrek dokuları üzerindeki toksik etkilerinin incelenmesi ve meydana gelen histopatolojik değişikliklerin araştırılmasıdır. Gereç ve yöntem: Bu çalışmada, daha önceden prenatal dönemden itibaren içme suyu ile 0, 30 ve 100 ppm F dozları verilen ve 1., 3. ile 5. aylarda sakrifiye edilen sıçanlara ait böbrek dokuları kullanılmıştır. Histolojik değerlendirme için hematoksilen ve eozin (H&E), immünohistokimyasal inceleme için ise TUNEL yöntemi ile boyama yapılarak, tubulus ve glomerulus bölgelerinde meydana gelen apoptozis değerlendirilmiştir. Bulgular: 100 ppm F gruplarında tespit edilen apoptotik hücre sayısı, kontrol grubu ve 30 ppm F grubununa kıyasla anlamlı derecede yüksek bulunmuştur. Ayrıca hem tubulus hem de glomerulus bölgelerinde, tüm sıçanlarda böbrek dokularının 5. ay apoptozis değerlerinin, 1. ve 3. ay değerlerinden anlamlı şekilde olduğu tespit edilmiştir. Böbrek dokularında yapılan histolojik incelemelerde ise içme suyundaki F miktarının artması ile tübüler dilatasyon, glomeruluslarda atrofi ve nekroz alanları, vasküler konjesyon ve hemorajik kanama odakları gözlemlenmiştir. Sonuç: Yüksek F konsantrasyonunda sıçan böbrek dokularında apoptoziste artış ve belirgin bir yıkım gözlenmiştir. Anahtar sözcükler: fluor, böbrek, TUNEL, apoptozis Investigation of apoptosis in renal tissues of rats exposed from prenatal period to systemic fluoride ABSTRACT Aim: The aim of this study was to evaluate fluoride (F) induced toxic effects and histopathological changes on renal tissues of rats exposed to different concentrations of fluoride pre and postnatally. Material and Methods: Kidneys of the rats that had received 0, 30 and 100 ppm F in drinking water starting from the prenatal period and sacrificed at first, third and fifth months were used in this study. Renal tissues were sectioned and stained with hematoxylin and eosin (H&E) for histological analysis and with TUNEL technique for immunohistochemical evaluations of apoptosis in glomerular and tubular sections. Results: In the 100 ppm F group, the number of glomerular and tubular apoptotic cells were significantly higher than control and 30 ppm F groups. In all groups, higher apoptosis was detected in the 5th month than the 1st and 3rd months in glomerular and tubular sections and the differences were statistically significant. The transverse section of kidney of the 5th month rat group showed tubular dilatations, glomerular atrophies and necrosis areas, vascular congestions and hemorrhagic regions. Conclusion: High F doses administered in drinking water caused severe apoptosis and a marked destruction in rat kidney tissues. Key Words: fluoride, kidney, TUNEL, apoptosis

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Diş Hekimliği

Bölüm

Araştırma Makalesi

Yazarlar

Serhat Karaca Bu kişi benim
0000-0003-1588-1480
Andorra

Şirin Güner Onur Bu kişi benim
0000-0002-6890-3500
Andorra

İlker Tinay Bu kişi benim
0000-0001-6768-9373
Andorra

Süheyla Uyar Bozkurt Bu kişi benim
0000-0002-5947-947X
Andorra

Ali – Orcid Number: Menteş Bu kişi benim
0000-0002-2778-6803
Andorra

Yayımlanma Tarihi

15 Ekim 2020

Gönderilme Tarihi

2 Mart 2020

Kabul Tarihi

21 Ağustos 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 30 Sayı: 4

DOI

https://doi.org/10.17567/ataunidfd.783461

IZ

https://izlik.org/JA53NH53XP

Kaynak Göster

RIS / Bibtex

APA

Karaca, S., Güner Onur, Ş., Tinay, İ., Uyar Bozkurt, S., & Menteş, A. – O. N. (2020). PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 30(4), 5711-578. https://doi.org/10.17567/ataunidfd.783461

AMA

1.Karaca S, Güner Onur Ş, Tinay İ, Uyar Bozkurt S, Menteş A – ON. PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ. Ata Diş Hek Fak Derg. 2020;30(4):5711-578. doi:10.17567/ataunidfd.783461

Chicago

Karaca, Serhat, Şirin Güner Onur, İlker Tinay, Süheyla Uyar Bozkurt, ve Ali – Orcid Number: Menteş. 2020. “PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 (4): 5711-578. https://doi.org/10.17567/ataunidfd.783461.

EndNote

Karaca S, Güner Onur Ş, Tinay İ, Uyar Bozkurt S, Menteş A – ON (01 Ekim 2020) PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 4 5711–578.

IEEE

[1]S. Karaca, Ş. Güner Onur, İ. Tinay, S. Uyar Bozkurt, ve A. – O. N. Menteş, “PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ”, Ata Diş Hek Fak Derg, c. 30, sy 4, ss. 5711–578, Eki. 2020, doi: 10.17567/ataunidfd.783461.

ISNAD

Karaca, Serhat - Güner Onur, Şirin - Tinay, İlker - Uyar Bozkurt, Süheyla - Menteş, Ali – Orcid Number: “PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30/4 (01 Ekim 2020): 5711-578. https://doi.org/10.17567/ataunidfd.783461.

JAMA

1.Karaca S, Güner Onur Ş, Tinay İ, Uyar Bozkurt S, Menteş A – ON. PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ. Ata Diş Hek Fak Derg. 2020;30:5711–578.

MLA

Karaca, Serhat, vd. “PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 30, sy 4, Ekim 2020, ss. 5711-578, doi:10.17567/ataunidfd.783461.

Vancouver

1.Serhat Karaca, Şirin Güner Onur, İlker Tinay, Süheyla Uyar Bozkurt, Ali – Orcid Number: Menteş. PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ. Ata Diş Hek Fak Derg. 01 Ekim 2020;30(4):5711-578. doi:10.17567/ataunidfd.783461