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MICROARRAY TECHNOLOGY AND USE IN DENTISTRY

Yıl 2013, Cilt 23, 55 - 62, 01.07.2013

Öz

In recent years, the advances in the computer technologies and molecular biology techniques opened the door to use these techiques together. Microarray technology is based on using new methods which provide the detection of many genes at the same time within one sample. Although the microarray technologies are used particularly in medical science, they are also, used in biological, microbiological and genetical sciences. There are many types of microarray technologies which are used for different purposes. Currently, using microarray techniques bloomed in dental research area. This technique seemed to be very advantageous, as many genes can be detected at the same time unit in the studies which are related with genetic susceptibility. It is considered that, microarray technology is a guide method in the diagnosis of diseases and development of new treatment procedures. The purpose of this review is to make known the microarray method and give information how to use this bioinformatic methods in dentistry.

Kaynakça

  • Nares S. The genetic relationship to periodontal disease. Periodontol 2000 2003;32:36-49.
  • Landau DA, Slack FJ. MicroRNAs in mutagenesis, genomic instability, and DNA repair. Semin Oncol 2011;38:743-51.
  • Tefferi A, Bolander ME, Ansell SM, Wieben ED, Spelsberg TC. Primer on medical genomics. Part III: Microarray experiments and data analysis. Mayo Clin Proc 2002;77:927-40.
  • Çetiner DE, M. Periodontal Teşhiste Hastalığa Ait Potansiyel Markerlar. Atatürk Üniv. Diş. Hek. Fak. Dergisi 2000;10:66-72
  • Bier FF, von Nickisch-Rosenegk M, EhrentreichForster E, Reiss E, Henkel J, Strehlow R, et al. DNA microarrays. Adv Biochem Eng Biotechnol 2008;109:433-53.
  • Liu H, Bebu I, Li X. Microarray probes and probe sets. Front Biosci (Elite Ed) 2010;2:325-38.
  • Schena M, Shalon D, Davis RW, Brown PO. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995;270:467-70.
  • Fodor SP, Read JL, Pirrung MC, Stryer L, Lu AT, Solas D. Light-directed, spatially addressable parallel chemical synthesis. Science 1991;251:767
  • Offenbacher S, Heasman PA, Collins JG. Modulation of host PGE2 secretion as a determinant of periodontal disease expression. J Periodontol 1993;64(5 Suppl):432-44.
  • Iida K, Nishimura I. Gene expression profiling by DNA microarray technology. Crit Rev Oral Biol Med 2002;13:35-50.
  • Yula ED, Ö. Nanotıp, mikrodizilimler ve klinik mikrobiyolojide kullanımları. Dicle Tıp Dergisi 2010;
  • Miller MB, Tang YW. Basic concepts of microarrays and potential applications in clinical microbiology. Clin Microbiol Rev 2009;22:611-33.
  • Friend SH, Stoughton RB. The magic of microarrays. Sci Am 2002;286:44-9, 53.
  • Howbrook DN, van der Valk AM, O'Shaughnessy MC, Sarker DK, Baker SC, Lloyd AW. Developments in microarray technologies. Drug Discov Today 2003;8:642-51.
  • Coppee JY. Do DNA microarrays have their future behind them? Microbes Infect 2008;10:1067-71.
  • Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, et al. Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 1998;9:3273-97.
  • Kim SH, Kim YS, Lee SY, Kim KH, Lee YM, Kim WK, et al. Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow. J Periodontal Implant Sci 2011;41:192200.
  • Mori G, Brunetti G, Oranger A, Carbone C, Ballini A, Lo Muzio L, et al. Dental pulp stem cells: osteogenic differentiation and gene expression. Ann N Y Acad Sci 2011;1237:47-52.
  • Parkar MH, Tonetti M. Gene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: analysis using cDNA arrays. J Periodontol 2004;75:1539-46.
  • Luan X, Ito Y, Holliday S, Walker C, Daniel J, Galang TM, et al. Extracellular matrix-mediated tissue remodeling following axial movement of teeth. J Histochem Cytochem 2007;55:127-40.
  • Jenkins SG, Schuetz AN. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc 2012;87:290-308.
  • Slavkin HC. Human genome, oral infection/ systemic diseases & the future of clinical dentistry. Ann Dent 2001; 5: 12-21.
  • Kuo WP, Whipple ME, Sonis ST, Ohno-Machado L, Jenssen TK. Gene expression profiling by DNA microarrays and its application to dental research. Oral Oncol 2002; 38: 650-6.
  • Radhakrishnan R, Solomon M, Satyamoorthy K, Martin LE, Lingen MW. Tissue microarray - a highthroughput molecular analysis in head and neck cancer. J Oral Pathol Med 2008;37:166-76.
  • Alevizos I, Mahadevappa M, Zhang X, Ohyama H, Kohno Y, Posner M, et al. Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis. Oncogene 2001;20:6196-204.
  • Konkimalla VB, Suhas VL, Chandra NR, Gebhart E, Efferth T. Diagnosis and therapy of oral squamous cell carcinoma. Expert Rev Anticancer Ther 2007;7:317-29.
  • Kuo WP, Whipple ME, Jenssen TK, Todd R, Epstein JB, Ohno-Machado L, et al. Microarrays and clinical dentistry. J Am Dent Assoc 2003;134:456-62.
  • Kuo WP, Jenssen TK, Park PJ, Lingen MW, Hasina R, Ohno-Machado L. Gene expression levels in different stages of progression in oral squamous cell carcinoma. Proc AMIA Symp 2002:415-9.
  • Duarte PM, da Rocha M, Sampaio E, Mestnik MJ, Feres M, Figueiredo LC, et al. Serum levels of cytokines in subjects with generalized chronic and aggressive periodontitis before and after nonsurgical periodontal therapy: a pilot study. J Periodontol 2010;81:1056-63.
  • Eley BM, Cox SW. Advances in periodontal diagnosis. 9. Potential markers of cell death and tissue degradation. Br Dent J 1998;184:427-30.
  • Tüter G. Periodontal Patogenezin Değerlendirilmesinde Kullanılan Yöntemler. Gazi Üniv. Diş. Hek. Fak. Dergisi 2001;18:147-53.
  • Sakai A, Ohshima M, Sugano N, Otsuka K, Ito K. Profiling the cytokines in gingival crevicular fluid using a cytokine antibody array. J Periodontol 2006;77:856-64.
  • Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, et al. Initial sequencing and analysis of the human genome. Nature 2001;409:860-921.
  • Frye JG, Jesse T, Long F, Rondeau G, Porwollik S, McClelland M, et al. DNA microarray detection of antimicrobial resistance genes in diverse bacteria. Int J Antimicrob Agents 2006;27(2):138-51.
  • Duran-Pinedo AE, Paster B, Teles R, Frias-Lopez J. Correlation network analysis applied to complex biofilm communities. PLoS One 2011;6:e28438.
  • Papapanou PN, Behle JH, Kebschull M, Celenti R, Wolf DL, Handfield M, et al. Subgingival bacterial colonization profiles correlate with gingival tissue gene expression. BMC Microbiol 2009;9:221.
  • Davanian H, Bage T, Lindberg J, Lundeberg J, H QC, Sallberg Chen M, et al. Signaling pathways involved in the regulation of TNFalpha-induced tolllike receptor 2 expression in human gingival fibroblasts. Cytokine 2012.
  • Vardar-Sengul S, Arora S, Baylas H, Mercola D. Expression profile of human gingival fibroblasts induced by interleukin-1beta reveals central role of nuclear factor-kappa B in stabilizing human gingival fibroblasts during inflammation. J Periodontol 2009;80:833-49.
  • Orban JE, Stallard RE. Gingival crevicular fluid: a reliable predictor of gingival health? J Periodontol 1969;40:231-5.
  • Gorska R, Gregorek H, Kowalski J, LaskusPerendyk A, Syczewska M, Madalinski K. Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol 2003;30:1046-52.
  • Chandler HL, Colitz CM. Molecular biology for the clinician: understanding current methods. J Am Anim Hosp Assoc 2006;42:326-35.
  • Rimini R, Schwenk JM, Sundberg M, Sjoberg R, Klevebring D, Gry M, et al. Validation of serum protein profiles by a dual antibody array approach. J Proteomics 2009;73:252-66.
  • Varnum SM, Woodbury RL, Zangar RC. A protein microarray ELISA for screening biological fluids. Methods Mol Biol 2004;264:161-72.
  • Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 2009;80:1421-32.
  • Preza D, Olsen I, Willumsen T, Boches SK, Cotton SL, Grinde B, et al. Microarray analysis of the microflora of root caries in elderly. Eur J Clin Microbiol Infect Dis 2009;28:509-17.
  • Asikainen S, Dogan B, Turgut Z, Paster BJ, Bodur A, Oscarsson J. Specified species in gingival crevicular fluid predict bacterial diversity. PLoS One 2010;5:e13589.
  • Rody WJ, Jr., Akhlaghi H, Akyalcin S, Wiltshire WA, Wijegunasinghe M, Filho GN. Impact of orthodontic retainers on periodontal health status assessed by biomarkers in gingival crevicular fluid. Angle Orthod 2011;81:1083-9.
  • Ramseier CA, Kinney JS, Herr AE, Braun T, Sugai JV, Shelburne CA, et al. Identification of pathogen and host-response markers correlated with periodontal disease. J Periodontol 2009;80:436-46.
  • Wang PL, Ohura K, Fujii T, Oido-Mori M, Kowashi Y, Kikuchi M, et al. DNA microarray analysis of human gingival fibroblasts from healthy and inflammatory gingival tissues. Biochem Biophys Res Commun 2003;305:970-3.
  • Wright JT, Hart TC. The genome projects: implications for dental practice and education. J Dent Educ 2002;66:659-71.
  • Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83.
  • Kashi Y, King D, Soller M. Simple sequence repeats as a source of quantitative genetic variation. Trends Genet 1997;13:74-8.
  • Bryant PA, Venter D, Robins-Browne R, Curtis N. Chips with everything: DNA microarrays in infectious diseases. Lancet Infect Dis 2004;4:100
  • Schena MD, R.W. Genes, Genomes, and Chips. Eds; Schena M. DNA Microarrays A Practical Approach. New York: Oxford University Press; 200 1-16. Karaca MA, A.N. Array gen expresyon teknolojisi ve bitkisel üretimde kullanımı. Alatarım 2004;3. Yazışma Adresi Dt. Özge ŞİMŞEK
  • Primadent Ağız, Diş Sağlığı ve İmplantoloji Kliniği Mahmut Yesari Sokak 3/A Çankaya/ ANKARA Tel: 0312-438 44 38 Fax: 0312-441 66 80 e-mail: ozgerol_83@hotmail.com

MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI

Yıl 2013, Cilt 23, 55 - 62, 01.07.2013

Öz

Son yıllarda bilgisayar teknolojileri ile moleküler biyoloji tekniklerindeki ilerlemeler her iki tekniğin birlikte kullanılmasına olanak tanımaktadır. Moleküler biyolojik yöntemlerdeki gelişmeler ile son yıllarda mikroarray teknolojisi ön plana çıkmıştır. Mikroarray teknolojisi, geleneksel yöntemlerin aksine aynı anda pek çok genin araştırılmasına olanak sağlayan yeni analitiksel metodlar sunmaktadır. Bu teknik özellikle tıp alanı olmakla birlikte; biyoloji, mikrobiyoloji, genetik gibi pek çok alanda da kullanılmaktadır. Farklı amaçlara uygun pek çok mikroarray tipi mevcuttur. Diş hekimliğinde mikroarray tekniğinin kullanımı son yıllarda yeni gelişmekte olan bir sistemdir. Bu yöntem ile aynı anda pek çok genin incelenebilmesinden dolayı genetik yatkınlıkla ilgili birçok çalışma için bu tekniğin avantajlı olacağı düşünülmektedir. Hastalıkların teşhisi ve yeni tedavi stratejilerinin geliştirilmesinde mikroarray teknolojilerinin yol gösterici olacağı düşünülmektedir. Bu derlemenin amacı mikroarray yöntemini tanıtmak ve bu yeni gelişmekte olan biyoinformatiksel yöntemin diş hekimliği alanında kullanımı hakkında bilgi vermektir.

Kaynakça

  • Nares S. The genetic relationship to periodontal disease. Periodontol 2000 2003;32:36-49.
  • Landau DA, Slack FJ. MicroRNAs in mutagenesis, genomic instability, and DNA repair. Semin Oncol 2011;38:743-51.
  • Tefferi A, Bolander ME, Ansell SM, Wieben ED, Spelsberg TC. Primer on medical genomics. Part III: Microarray experiments and data analysis. Mayo Clin Proc 2002;77:927-40.
  • Çetiner DE, M. Periodontal Teşhiste Hastalığa Ait Potansiyel Markerlar. Atatürk Üniv. Diş. Hek. Fak. Dergisi 2000;10:66-72
  • Bier FF, von Nickisch-Rosenegk M, EhrentreichForster E, Reiss E, Henkel J, Strehlow R, et al. DNA microarrays. Adv Biochem Eng Biotechnol 2008;109:433-53.
  • Liu H, Bebu I, Li X. Microarray probes and probe sets. Front Biosci (Elite Ed) 2010;2:325-38.
  • Schena M, Shalon D, Davis RW, Brown PO. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995;270:467-70.
  • Fodor SP, Read JL, Pirrung MC, Stryer L, Lu AT, Solas D. Light-directed, spatially addressable parallel chemical synthesis. Science 1991;251:767
  • Offenbacher S, Heasman PA, Collins JG. Modulation of host PGE2 secretion as a determinant of periodontal disease expression. J Periodontol 1993;64(5 Suppl):432-44.
  • Iida K, Nishimura I. Gene expression profiling by DNA microarray technology. Crit Rev Oral Biol Med 2002;13:35-50.
  • Yula ED, Ö. Nanotıp, mikrodizilimler ve klinik mikrobiyolojide kullanımları. Dicle Tıp Dergisi 2010;
  • Miller MB, Tang YW. Basic concepts of microarrays and potential applications in clinical microbiology. Clin Microbiol Rev 2009;22:611-33.
  • Friend SH, Stoughton RB. The magic of microarrays. Sci Am 2002;286:44-9, 53.
  • Howbrook DN, van der Valk AM, O'Shaughnessy MC, Sarker DK, Baker SC, Lloyd AW. Developments in microarray technologies. Drug Discov Today 2003;8:642-51.
  • Coppee JY. Do DNA microarrays have their future behind them? Microbes Infect 2008;10:1067-71.
  • Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, et al. Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 1998;9:3273-97.
  • Kim SH, Kim YS, Lee SY, Kim KH, Lee YM, Kim WK, et al. Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow. J Periodontal Implant Sci 2011;41:192200.
  • Mori G, Brunetti G, Oranger A, Carbone C, Ballini A, Lo Muzio L, et al. Dental pulp stem cells: osteogenic differentiation and gene expression. Ann N Y Acad Sci 2011;1237:47-52.
  • Parkar MH, Tonetti M. Gene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: analysis using cDNA arrays. J Periodontol 2004;75:1539-46.
  • Luan X, Ito Y, Holliday S, Walker C, Daniel J, Galang TM, et al. Extracellular matrix-mediated tissue remodeling following axial movement of teeth. J Histochem Cytochem 2007;55:127-40.
  • Jenkins SG, Schuetz AN. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc 2012;87:290-308.
  • Slavkin HC. Human genome, oral infection/ systemic diseases & the future of clinical dentistry. Ann Dent 2001; 5: 12-21.
  • Kuo WP, Whipple ME, Sonis ST, Ohno-Machado L, Jenssen TK. Gene expression profiling by DNA microarrays and its application to dental research. Oral Oncol 2002; 38: 650-6.
  • Radhakrishnan R, Solomon M, Satyamoorthy K, Martin LE, Lingen MW. Tissue microarray - a highthroughput molecular analysis in head and neck cancer. J Oral Pathol Med 2008;37:166-76.
  • Alevizos I, Mahadevappa M, Zhang X, Ohyama H, Kohno Y, Posner M, et al. Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis. Oncogene 2001;20:6196-204.
  • Konkimalla VB, Suhas VL, Chandra NR, Gebhart E, Efferth T. Diagnosis and therapy of oral squamous cell carcinoma. Expert Rev Anticancer Ther 2007;7:317-29.
  • Kuo WP, Whipple ME, Jenssen TK, Todd R, Epstein JB, Ohno-Machado L, et al. Microarrays and clinical dentistry. J Am Dent Assoc 2003;134:456-62.
  • Kuo WP, Jenssen TK, Park PJ, Lingen MW, Hasina R, Ohno-Machado L. Gene expression levels in different stages of progression in oral squamous cell carcinoma. Proc AMIA Symp 2002:415-9.
  • Duarte PM, da Rocha M, Sampaio E, Mestnik MJ, Feres M, Figueiredo LC, et al. Serum levels of cytokines in subjects with generalized chronic and aggressive periodontitis before and after nonsurgical periodontal therapy: a pilot study. J Periodontol 2010;81:1056-63.
  • Eley BM, Cox SW. Advances in periodontal diagnosis. 9. Potential markers of cell death and tissue degradation. Br Dent J 1998;184:427-30.
  • Tüter G. Periodontal Patogenezin Değerlendirilmesinde Kullanılan Yöntemler. Gazi Üniv. Diş. Hek. Fak. Dergisi 2001;18:147-53.
  • Sakai A, Ohshima M, Sugano N, Otsuka K, Ito K. Profiling the cytokines in gingival crevicular fluid using a cytokine antibody array. J Periodontol 2006;77:856-64.
  • Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, et al. Initial sequencing and analysis of the human genome. Nature 2001;409:860-921.
  • Frye JG, Jesse T, Long F, Rondeau G, Porwollik S, McClelland M, et al. DNA microarray detection of antimicrobial resistance genes in diverse bacteria. Int J Antimicrob Agents 2006;27(2):138-51.
  • Duran-Pinedo AE, Paster B, Teles R, Frias-Lopez J. Correlation network analysis applied to complex biofilm communities. PLoS One 2011;6:e28438.
  • Papapanou PN, Behle JH, Kebschull M, Celenti R, Wolf DL, Handfield M, et al. Subgingival bacterial colonization profiles correlate with gingival tissue gene expression. BMC Microbiol 2009;9:221.
  • Davanian H, Bage T, Lindberg J, Lundeberg J, H QC, Sallberg Chen M, et al. Signaling pathways involved in the regulation of TNFalpha-induced tolllike receptor 2 expression in human gingival fibroblasts. Cytokine 2012.
  • Vardar-Sengul S, Arora S, Baylas H, Mercola D. Expression profile of human gingival fibroblasts induced by interleukin-1beta reveals central role of nuclear factor-kappa B in stabilizing human gingival fibroblasts during inflammation. J Periodontol 2009;80:833-49.
  • Orban JE, Stallard RE. Gingival crevicular fluid: a reliable predictor of gingival health? J Periodontol 1969;40:231-5.
  • Gorska R, Gregorek H, Kowalski J, LaskusPerendyk A, Syczewska M, Madalinski K. Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol 2003;30:1046-52.
  • Chandler HL, Colitz CM. Molecular biology for the clinician: understanding current methods. J Am Anim Hosp Assoc 2006;42:326-35.
  • Rimini R, Schwenk JM, Sundberg M, Sjoberg R, Klevebring D, Gry M, et al. Validation of serum protein profiles by a dual antibody array approach. J Proteomics 2009;73:252-66.
  • Varnum SM, Woodbury RL, Zangar RC. A protein microarray ELISA for screening biological fluids. Methods Mol Biol 2004;264:161-72.
  • Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 2009;80:1421-32.
  • Preza D, Olsen I, Willumsen T, Boches SK, Cotton SL, Grinde B, et al. Microarray analysis of the microflora of root caries in elderly. Eur J Clin Microbiol Infect Dis 2009;28:509-17.
  • Asikainen S, Dogan B, Turgut Z, Paster BJ, Bodur A, Oscarsson J. Specified species in gingival crevicular fluid predict bacterial diversity. PLoS One 2010;5:e13589.
  • Rody WJ, Jr., Akhlaghi H, Akyalcin S, Wiltshire WA, Wijegunasinghe M, Filho GN. Impact of orthodontic retainers on periodontal health status assessed by biomarkers in gingival crevicular fluid. Angle Orthod 2011;81:1083-9.
  • Ramseier CA, Kinney JS, Herr AE, Braun T, Sugai JV, Shelburne CA, et al. Identification of pathogen and host-response markers correlated with periodontal disease. J Periodontol 2009;80:436-46.
  • Wang PL, Ohura K, Fujii T, Oido-Mori M, Kowashi Y, Kikuchi M, et al. DNA microarray analysis of human gingival fibroblasts from healthy and inflammatory gingival tissues. Biochem Biophys Res Commun 2003;305:970-3.
  • Wright JT, Hart TC. The genome projects: implications for dental practice and education. J Dent Educ 2002;66:659-71.
  • Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83.
  • Kashi Y, King D, Soller M. Simple sequence repeats as a source of quantitative genetic variation. Trends Genet 1997;13:74-8.
  • Bryant PA, Venter D, Robins-Browne R, Curtis N. Chips with everything: DNA microarrays in infectious diseases. Lancet Infect Dis 2004;4:100
  • Schena MD, R.W. Genes, Genomes, and Chips. Eds; Schena M. DNA Microarrays A Practical Approach. New York: Oxford University Press; 200 1-16. Karaca MA, A.N. Array gen expresyon teknolojisi ve bitkisel üretimde kullanımı. Alatarım 2004;3. Yazışma Adresi Dt. Özge ŞİMŞEK
  • Primadent Ağız, Diş Sağlığı ve İmplantoloji Kliniği Mahmut Yesari Sokak 3/A Çankaya/ ANKARA Tel: 0312-438 44 38 Fax: 0312-441 66 80 e-mail: ozgerol_83@hotmail.com

Ayrıntılar

Birincil Dil Türkçe
Konular Diş Hekimliği
Bölüm Makaleler
Yazarlar

Dt. Özge ŞİMŞEK Bu kişi benim

Yayımlanma Tarihi 1 Temmuz 2013
Yayınlandığı Sayı Yıl 2013, Cilt 23, Sayı

Kaynak Göster

Bibtex @araştırma makalesi { ataunidfd31453, journal = {Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi}, issn = {1300-9044}, eissn = {2667-5161}, address = {}, publisher = {Atatürk Üniversitesi}, year = {2013}, volume = {23}, pages = {55 - 62}, title = {MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI}, key = {cite}, author = {Şimşek, Dt. Özge} }
APA Şimşek, D. Ö. (2013). MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI . Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi , Supplement 7 , 55-62 . Retrieved from https://dergipark.org.tr/tr/pub/ataunidfd/issue/2465/31453
MLA Şimşek, D. Ö. "MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI" . Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 23 (2013 ): 55-62 <https://dergipark.org.tr/tr/pub/ataunidfd/issue/2465/31453>
Chicago Şimşek, D. Ö. "MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 23 (2013 ): 55-62
RIS TY - JOUR T1 - MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI AU - Dt. ÖzgeŞimşek Y1 - 2013 PY - 2013 N1 - DO - T2 - Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi JF - Journal JO - JOR SP - 55 EP - 62 VL - 23 IS - SN - 1300-9044-2667-5161 M3 - UR - Y2 - 2022 ER -
EndNote %0 Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI %A Dt. Özge Şimşek %T MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI %D 2013 %J Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi %P 1300-9044-2667-5161 %V 23 %N %R %U
ISNAD Şimşek, Dt. Özge . "MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 23 / (Temmuz 2013): 55-62 .
AMA Şimşek D. Ö. MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI. Ata Diş Hek Fak Derg. 2013; 23: 55-62.
Vancouver Şimşek D. Ö. MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2013; 23: 55-62.
IEEE D. Ö. Şimşek , "MİKROARRAY TEKNOLOJİSİ VE DİŞ HEKİMLİĞİ’NDE KULLANIMI", Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 23, ss. 55-62, Tem. 2013

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