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Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran bireylerin aerobik bakteriyel florasının mikrobiyal teşhis sistemi kullanılarak saptanması

Yıl 2012, Cilt: 2012 Sayı: 1, 26 - 30, 01.01.2012

Öz

-

Kaynakça

  • Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 1998; 62: 71-109.
  • Thylstrup A, Fejerkov O. Textbook of Clinical Cariology. 2ed.Copenhagen; Munsgaard:1994.p. 5-50.
  • Gabris K, Nagy G, Madlena M, Denes Z, Marton S, Keszthelyi G, Banoczy J. Associations between microbiological and salivary caries activity tests and caries experience in Hungarian adolescents. Caries Res 1999; 33:191-195.
  • Tukia-Kulmana H, Tenovuo J: Intra- and inter- individual variation in salivary flow rate, buffer effect, lactobacilli, and mutans streptococci among 11-to 12-year-old school children. Acta Odontol Scand 1993; 51: 31-37.
  • Beighton D, Manji F, Baelum V, Fejerskov O, Johndon Nw, Wilton JMA. Associations between salivary streptococcus sobrinus, lactobacilli, and caries experience in Kenyan adolescents. J Dent Res 1989; 68:1242-1246. streptococcus mutans,
  • Angulo M, Gabanas B, Camporeale N, Emilson CG. Dental microorganisms in Uruguayan preschool children. Acta Odontol Scand 1999; 57: 301-305.
  • Liena-Puy MC, Montanana-Llorens C, Forner- Navarro L. Cariogenic oral flora and its relation to dental caries. J Dent Child 2000; Jan-feb: 42-46.
  • Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott’s Diagnostic Microbiology.11 ed. St. Louis, Missouri, USA ; Mosby Inc :1998.p. 1068 .
  • Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology .9 ed. Baltimore, Maryland, USA; Lippincott Williams & Wilkins: 1994.p 787.
  • Odumeru JA, Steele M, Fruhner L, Larkin C, Jiang JD, Mann E, McNab WB. Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. J Clin Microbiol 1999; 37: 944-949.
  • Miller I, Berger T. Bacteria identification by gas chromatography of whole cell fatty acids. Hewlett-Packard Gas Chromatography Application Note 228-238. Hewlett-Packard Co. Alto, CA:1985.p 8. Sahin 12. F. Detection, identification and
  • characterization of strains of Xanthomonas
  • campestris pv. vesicatoria by traditional and
  • molecular methods, and resistance in Capsicum
  • species to Xanthomonas campestris pv. vesicatoria
  • pepper race 6. The Ohio State University, 182 p. (Ph.D. Thesis).
  • Sasser M. Identification of bacteria through fatty acid analysis. in Methods in Phytobacteriology. Z. Klement, K. Rudolph, and D. Sands, eds. Budapest ;Akademiai Kiado: 1990.p.199-204.
  • Sherlock Microbial Identification System (version 4.0), MIS Operating manual, MIDI, inc, Newyork, DE, USA,p 145 .
  • Yang P, Vauterin L, VanCanneyt M, Swings J, Kersters K. Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas. Syst Appl Microbiol 1993; 16: 47-71.
  • Stead DE. Identification of bacteria by computer-assisted fatty acid profiling. Acta Hortic 1988; 225: 39-46.
  • World Health Organizations. Oral Health Surveys- Basic Methods.4th edition. Geneva: WHO: 1997.
  • Gudkina J, Brinkmane A .Caries experience in relation to oral hygiene, salivary cariogenic microflora, buffer capacity and secretion rate in 6- year olds and 12 year olds in RigaStomatologija, Baltic Dental and Maxillofacial Journal 2008;10:76- 80.
  • Willcox MDP, Drucker DB, Green RM. In vivo dental plaque-forming ability and cariogenicity of the bacterium Streptococcus bovis in gnotobiotic rats. Arch Oral Biol 1990;35:163-166.
  • Crawford I, Russell C. Streptococci isolated from the bloodstream and gingival crevice of man. J. Med. Microbiol. 1986;16, 263-269.
  • De Soet JJ, Nyvad B, Kilian M. Strain-related acid production by oral srteptococci. Caries Res 2000; 34: 486-490.
  • Brailsford SR, Byrne RW, Adams S, Zoitopoulos L, Allison C, Beighton D. Investigation of the aciduric microflora of plaque. Caries Res 1999; 33: 290.
  • Rubinstein I, Pedersen GW. Bacillus species are present in chewing tobacco sold in the United States and evoke plasma exudation from the oral mucosa. Clin Diagn Lab Immunol 2002; 9: 1057- 1060.

Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system

Yıl 2012, Cilt: 2012 Sayı: 1, 26 - 30, 01.01.2012

Öz

Bu çalışmanın amacı, Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran hastaların tükürük aerobik bakteri florasını tanımlamaktır. Yöntem: DMFT indeksi 9-15 arasındaki 20 sağlıklı bireyin sitümüle edilmemiş tükürükleri toplandı.Toplam bakteri florası iki seçici olmayan medya, Nutrient Agar ve Sensitive Agar üzerindeki tükürük örneklerinden toplanmıştır. Izole bakteriyel organizmalar, fatty acid methyl ester (FAME) analizine dayanan Microbial Identification System (MIS; MIDI Inc., Newark, Del.) kullanılarak sınıflandırıldı . Sonuçlar. 75 bakteri suş izole edildi. Bunlardan, 67’sinin , 16 cins ve 34 türe ait olduğu saptanırken Kalan suşlar tanımlanamadı. En çok bulunan bakteri Streptococcus iken takip edenler Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helikobakter, Actinomadura, Kocuria, Pseudomonas ve Cellulomonas oldu. Bilgilerimize dayanarak bu çalışma, bakteri türlerinden Xanthobacter Psychrobacter-phenylpyruvicus’u ağız florasında tanımlayan ilk literatürdür. Agilis, Actinomadura yumaensis ve Sonuç: Bu Çalışma;tükürük aerobik bakterilerinin tespiti için MIS’den yararlanılan ilk çalışmadır. Bizim sonuçlarımız MIS’in tükürük bakterinin tespiti için verimli ve hızlı ve doğru bir yöntem olduğunu desteklemektedir; ancak MIS veritabanlarının özellikle ağız bakteri florası olanlarının geliştirilmesi gerekmektedir

Kaynakça

  • Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 1998; 62: 71-109.
  • Thylstrup A, Fejerkov O. Textbook of Clinical Cariology. 2ed.Copenhagen; Munsgaard:1994.p. 5-50.
  • Gabris K, Nagy G, Madlena M, Denes Z, Marton S, Keszthelyi G, Banoczy J. Associations between microbiological and salivary caries activity tests and caries experience in Hungarian adolescents. Caries Res 1999; 33:191-195.
  • Tukia-Kulmana H, Tenovuo J: Intra- and inter- individual variation in salivary flow rate, buffer effect, lactobacilli, and mutans streptococci among 11-to 12-year-old school children. Acta Odontol Scand 1993; 51: 31-37.
  • Beighton D, Manji F, Baelum V, Fejerskov O, Johndon Nw, Wilton JMA. Associations between salivary streptococcus sobrinus, lactobacilli, and caries experience in Kenyan adolescents. J Dent Res 1989; 68:1242-1246. streptococcus mutans,
  • Angulo M, Gabanas B, Camporeale N, Emilson CG. Dental microorganisms in Uruguayan preschool children. Acta Odontol Scand 1999; 57: 301-305.
  • Liena-Puy MC, Montanana-Llorens C, Forner- Navarro L. Cariogenic oral flora and its relation to dental caries. J Dent Child 2000; Jan-feb: 42-46.
  • Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott’s Diagnostic Microbiology.11 ed. St. Louis, Missouri, USA ; Mosby Inc :1998.p. 1068 .
  • Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology .9 ed. Baltimore, Maryland, USA; Lippincott Williams & Wilkins: 1994.p 787.
  • Odumeru JA, Steele M, Fruhner L, Larkin C, Jiang JD, Mann E, McNab WB. Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. J Clin Microbiol 1999; 37: 944-949.
  • Miller I, Berger T. Bacteria identification by gas chromatography of whole cell fatty acids. Hewlett-Packard Gas Chromatography Application Note 228-238. Hewlett-Packard Co. Alto, CA:1985.p 8. Sahin 12. F. Detection, identification and
  • characterization of strains of Xanthomonas
  • campestris pv. vesicatoria by traditional and
  • molecular methods, and resistance in Capsicum
  • species to Xanthomonas campestris pv. vesicatoria
  • pepper race 6. The Ohio State University, 182 p. (Ph.D. Thesis).
  • Sasser M. Identification of bacteria through fatty acid analysis. in Methods in Phytobacteriology. Z. Klement, K. Rudolph, and D. Sands, eds. Budapest ;Akademiai Kiado: 1990.p.199-204.
  • Sherlock Microbial Identification System (version 4.0), MIS Operating manual, MIDI, inc, Newyork, DE, USA,p 145 .
  • Yang P, Vauterin L, VanCanneyt M, Swings J, Kersters K. Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas. Syst Appl Microbiol 1993; 16: 47-71.
  • Stead DE. Identification of bacteria by computer-assisted fatty acid profiling. Acta Hortic 1988; 225: 39-46.
  • World Health Organizations. Oral Health Surveys- Basic Methods.4th edition. Geneva: WHO: 1997.
  • Gudkina J, Brinkmane A .Caries experience in relation to oral hygiene, salivary cariogenic microflora, buffer capacity and secretion rate in 6- year olds and 12 year olds in RigaStomatologija, Baltic Dental and Maxillofacial Journal 2008;10:76- 80.
  • Willcox MDP, Drucker DB, Green RM. In vivo dental plaque-forming ability and cariogenicity of the bacterium Streptococcus bovis in gnotobiotic rats. Arch Oral Biol 1990;35:163-166.
  • Crawford I, Russell C. Streptococci isolated from the bloodstream and gingival crevice of man. J. Med. Microbiol. 1986;16, 263-269.
  • De Soet JJ, Nyvad B, Kilian M. Strain-related acid production by oral srteptococci. Caries Res 2000; 34: 486-490.
  • Brailsford SR, Byrne RW, Adams S, Zoitopoulos L, Allison C, Beighton D. Investigation of the aciduric microflora of plaque. Caries Res 1999; 33: 290.
  • Rubinstein I, Pedersen GW. Bacillus species are present in chewing tobacco sold in the United States and evoke plasma exudation from the oral mucosa. Clin Diagn Lab Immunol 2002; 9: 1057- 1060.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Yrd. Doç. Dr. Nur Özdabak Bu kişi benim

Yrd. Doç. Dr. Serpil Karaoğlanoğlu Bu kişi benim

Yrd. Doç. Dr. Nilgün Akgül Bu kişi benim

Prof. Dr. Nilgün Seven Bu kişi benim

Yayımlanma Tarihi 1 Ocak 2012
Yayımlandığı Sayı Yıl 2012 Cilt: 2012 Sayı: 1

Kaynak Göster

APA Özdabak, Y. D. D. N., Karaoğlanoğlu, Y. D. D. S., Akgül, Y. D. D. N., Seven, P. D. N. (2012). Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 2012(1), 26-30.
AMA Özdabak YDDN, Karaoğlanoğlu YDDS, Akgül YDDN, Seven PDN. Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system. Ata Diş Hek Fak Derg. Ocak 2012;2012(1):26-30.
Chicago Özdabak, Yrd. Doç. Dr. Nur, Yrd. Doç. Dr. Serpil Karaoğlanoğlu, Yrd. Doç. Dr. Nilgün Akgül, ve Prof. Dr. Nilgün Seven. “Identification of Aerobic Bacterial Flora in Saliva of Subjects Who Apply to the Faculty of Dentistry in Atatürk University by Using Microbial Identification System”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2012, sy. 1 (Ocak 2012): 26-30.
EndNote Özdabak YDDN, Karaoğlanoğlu YDDS, Akgül YDDN, Seven PDN (01 Ocak 2012) Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2012 1 26–30.
IEEE Y. D. D. N. Özdabak, Y. D. D. S. Karaoğlanoğlu, Y. D. D. N. Akgül, ve P. D. N. Seven, “Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system”, Ata Diş Hek Fak Derg, c. 2012, sy. 1, ss. 26–30, 2012.
ISNAD Özdabak, Yrd. Doç. Dr. Nur vd. “Identification of Aerobic Bacterial Flora in Saliva of Subjects Who Apply to the Faculty of Dentistry in Atatürk University by Using Microbial Identification System”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2012/1 (Ocak 2012), 26-30.
JAMA Özdabak YDDN, Karaoğlanoğlu YDDS, Akgül YDDN, Seven PDN. Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system. Ata Diş Hek Fak Derg. 2012;2012:26–30.
MLA Özdabak, Yrd. Doç. Dr. Nur vd. “Identification of Aerobic Bacterial Flora in Saliva of Subjects Who Apply to the Faculty of Dentistry in Atatürk University by Using Microbial Identification System”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 2012, sy. 1, 2012, ss. 26-30.
Vancouver Özdabak YDDN, Karaoğlanoğlu YDDS, Akgül YDDN, Seven PDN. Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system. Ata Diş Hek Fak Derg. 2012;2012(1):26-30.

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