IMMUNOHISTOCHEMICAL EXAMINATION OF NUCLEAR FACTOR KAPA B ACTIVATION IN INDIVIDUALS WITH AGGRESSIVE PERIODONTITIS: STEREOLOGIC STUDY

Yıl 2010, Cilt: 2010 Sayı: 2, 84 - 91, 01.02.2010

Yrd. Doç. Dr. Taner Arabacı Doç. Dr. Yasin Çiçek Uzm. Mevlüt Albayrak Arş. Gör. Osman Nuri Keleş

Öz

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Anahtar Kelimeler

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AGRESİF PERİODONTİTİSLİ BİREYLERDE NÜKLEER FAKTÖR KAPPA B AKTİVASYONUN İMMUNOHİSTOKİMYASAL OLARAK İNCELENMESİ: STEREOLOJİK ÇALIŞMA

Öz

Bu çalışmanın amacı; agresif periodontitisli bireylerin diş eti dokularında Nükleer Faktör kappa B (NFкB) proteinlerinden p50 ve p65 alt ünitelerinin çekirdek ve sitoplazmik ekspresyonunun ve NF-кB inhibitörü olan İnhibitör kappa B’nin (IкB) sitoplazmik ekspresyonunun immunohistokimyasal ve stereolojik olarak araştırılmasıdır. Çalışma popülasyonunu, Atatürk Üniversitesi Diş Hekimliği Fakültesine 2006-2007 yılları arasında değişik nedenlerle müracaat eden 30 birey (15’i agresif periodontitisli ve 15’i periodontal olarak sağlıklı) oluşturdu. Tüm bireylerden lokal anestezi altında dişlerin bukkal dişeti bölgesinden biyopsi ile 1–2 mm diş eti dokusu alındı. Alınan dişeti dokuları hemen % 10’luk nötral tamponlu formalin içerisine konuldu ve sonra immunohistokimyasal boyamaya gönderildi. Elde edilen kesitler elektron mikroskobu altında incelendi ve stereolojik yöntem kullanılarak boyanan hücre sayısal değerleri karşılaştırmalarında Tek Yönlü Varyans Analizi ve Çoklu karşılaştırmalı en küçük önemli fark (LSD) testi (p=0.05) kullanıldı

Anahtar Kelimeler

Agresif periodontitis, nükleer faktör-kappa B, stereoloji

Kaynakça

• American Academy of Periodontology: Parameter on aggressive periodontitis. J Periodontol., 2000; 71 (Suppl.): 867-869.
• Barrington EP, Nevins M. Diagnosing periodontal diseases. J Am Dent Assoc 1990; 121: 460-464.
• Tonetti MS, Mombelli A. Early-onset periodontitis. Ann Periodontol 1999; 4: 39-52.
• American Academy of Periodontology. The new classification conditions. Ann Periodontol 1999; 4: 1-108. disease and
• Darveau RP, Tanner A, Page RC. The microbial challenge in periodontitis. Periodontol 2000 1997; 14: 12-32.
• Daniel MA, Van Dyke TE. Alterations in phagocyte function and periodontal infection. J Periodontol 1996; 67: 1070-1075.
• Yoshie H, Kobayashi T, Tai H, Galicia JC. The role of genetic polymorphisms in periodontitis. Periodontol 2000 2007; 43: 102-132.
• Li Q, Verma IM. NF-kB regulation in the immun system. Nature Reviews 2002; 2: 725- 735.
• Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S. Rel/NF-kB/IkB family: intimate tales of association and dissociation. Genes Dev 1995; 9: 2723-2735.
• Triphati P, Aggarwal A. NF-kB transcription factor: a key player in the generation of immune response. Current Science 2006; 90: 519-531.
• Sen R, Baltimore D. Inducibility of immunoglobulin enhancer-binding posttranslational mechanism. Cell 1986;47: 921- 928. NF-κB by a
• Baeuerle PA, Henkel T. Function and activation of NF-k/B in the immune system. Annu Rev Immunol 1994; 12: 141-179.
• Grimm S, Baeuerle PA. The inducible transcription factor NF-kB: structure-function relationship of its protein subunits. Biochem J 1999; 290: 297-308.
• Yıldız OG, Soyuer S, Soyuer I, Uçar K, Gundoğ M, Kaplan B, Özkan M. Gastrektomi sonrası adjuvan kemoradyoterapi uygulanan mide karsinomlu olgularda NF-kB’nin prognostik önemi. Türk Onkoloji Dergisi 2007; 22: 69-73.
• Imler JL, Hoffmann JA. Toll and Toll-like proteins: an ancient family of receptors family signaling infection. Rev. Immunogenet. 2000; 2: 294-304.
• Ghosh S, May MJ, Koop EB. NF-kB and Rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol 1998; 16: 225-260.
• Barnes PJ, Karin M. NF-kB. A pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 1997; 336: 1066-1071.
• Celancy RM, Amin AR, Abromson SB. The role of nitric oxide in inflammation and immunity. Arthritis&Rheumatism 1998; 41:1141-1151.
• Informational paper. Modulation of host response in periodontal therapy. J Periodontol 2002; 73:460- 470.
• Ataoğlu H, Alptekin NO, Haliloğlu S, Gürsel M, Ataoğlu T, Serpek B, Durmuş E. Interleukin 1β, tumor necrosis factor-α levels and neutrophil elastase activity in peri-implant crevicular fluid. Clin Oral Impl Res 2002; 13: 470-476.
• Erdemir EO, Duran I, Haliloğlu S. Effects of smoking on clinical parameters and the gingival crevicular fluid levels of IL-6 and TNF-α in patients with chronic periodontitis. J Clin Periodontol 2004; 31: 99-104.
• Pahl HL. Activators and target genes of Rel/NF-kB transcription factors. Oncogene 1999; 18: 6853- 6866.
• Milward MR, Chapple ILC, Wright HJ, Millard JL, Matthews JB, Cooper PR. Differantial activation of NF-kB and gene expression in oral epithelial cells by Experimental immunology 2007; 148: 307-324.
• Pihlstrom BL, Michalowicz BS, Jhonson NW. Periodontal diseases. Lancet 2005; 366: 1809- 1820. Clinical and
• Sillness J, Löe H. Periodontal disease in pregnancy II. Correlation of between oral hygiene and periodontal condition. Acta Odontol Scand. 1964; 22: 121-135.
• Löe H, Sillness J. Periodontal disease in pregnancy I. Prevalance and severity. Acta Odontol Scand. 1963; 21: 531-551.
• West MJ. The new stereological tools: Disector, Fractionator, intercepts and their use in pathological research and diagnosis. APMIS, 1988; 96: 857–881.
• International Workshop for a Classification of Periodontal Diseases and Conditions. Consensus report Periodontol., 4: 53, 1999.
• periodontitis. Ann.
• Zadeh HH, Nichols FC, Miyasaki KT. The role of the Actinobacillus Porphyromonas Periodontol. 2000 1999; 20: 239-288. to and
• actinomycetemcomitans gingivalis in periodontitis.
• Ambili R, Santhi WS, Janam P, Nandakumar K, Pillai RM. Expression of activated transcription factor Nuclear Factor-kB in periodontally diseased tissues. J Periodontol 2005; 76: 1148-1153.
• Newman MG, Takei HH, Carranza FA. Carranza’s clinical periodontology. Philedelphia London New York St Louis Sydney Toronto: W.B Saunders Company, 2002: 398-402.
• Armitage GC. Development of a classification system for periodontal disease and conditions. Ann Periodontol 1999; 4: 1-6.
• Flemmig TF. Periodontitis. Ann Periodontol 1999; 4: 32-37.
• Chen HY, Cox SW, Eley BM, Mäntylä P, Rönkä H, Sorsa T. Matrix metalloproteinase- 8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients. J Clin Periodontol 2000; 27: 366–369.
• Vis M, Havaardsholm EA, Haugeberg G, Uhlig T, Voskuyl AE, van de Stadt RJ, Dijkmans BAC, Woolf AD, Kvien TK, Lems WF. Evaluation of bone mineral density, bone metabolism, osteoprotegerin and receptor activator of the NFkB ligand serum levels during treatment with infliximab in patients with rheumatoid arthritis. Ann Rheum Dis 2006; 65: 1495-1499.
• Steeve KT, Marc P, Sandrine T, Dominique H, Yannick interrelations in bone resorption pathophysiology. Cytokine & Growth Factor Reviews 2004; 15: 49- 60. TNF-alpha/IL-1:
• Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CWO, Izumi Y, Taubman MA. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease. The American Journal of Pathology 2006; 169: 987-998.
• Ferrer I, Marti E, Lopez E, Tortosa A. NF-kB immunoreactivity is observed in association with βA4 diffuse plaques in patients with Alzheimer’s disease. Neuropathology and Applied Neurobiology 1998; 24: 271-277.
• Aggarwal BB. Nuclear factor-kB: The enemy within. Cancer Cell 2004; 6: 203-208.
• BelibasakisG.N., Johansson A., Wang Y, Chen C, Lagergard T, Kalfas S, Lerner U.H. Cytokine responses of human gingival fibroblasts to Actinobacillus actinomycetemcomitans cytolethal distending toxin. Cytokine 2005; 30: 56-63.
• Epinat JC, Gilmore TD. Diverse agents act at multiple levels to inhibit the Rel/NF-кB signal transduction pathway. Oncogene 1999; 18: 6896- 6909.
• Yin MJ, Yamamoto Y, Gaynor RB. The anti- inflammatory agents aspirin and salicylate inhibit the activity of IкB kinase-β. Nature 1998; 396: 77- 80.
• Makarov S. NF-кB as a therapeutic target in chronic inflammation: Recent advances. Mol Med Today 2000; 6: 441-448.
• Hirasawa M, Takada K, Makimura M, Otake S. Improvement of periodontal status by green tea catechin using a local delivery system: A clinical pilot study. J Periodont Res 2002; 37: 433-438.
• May MJ, D’Acquisto F, Madge LA, Glockner J, Pober JS, Ghosh S. Selective inhibition of NF-kappa B activation by a peptide that blocks the interaction of NEMO with the I kappa B kinase complex. Science 2000; 289: 1550-1554.