IN VITRO EVALUATION OF THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALERS WITH DIFFERENT CHEMICAL COMPOSITIONS

Yıl 2009, Cilt: 2009 Sayı: 2, 90 - 97, 01.02.2009

Dt. Ali Keleş Prof. Dr. Mustafa Köseoğlu Yrd. Doç.dr. K. Meltem Çolak Topçu Dt. Ö. Faruk Bayrak

Öz

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Anahtar Kelimeler

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FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

Öz

Bu çalışmamızın amacı, farklı sertleşme sürelerine sahip değişik içerikli kanal patlarının, 3T3 fare fibroblastları üzerinde oluşturdukları sitotoksisitenin in vitro olarak incelenmesi ve karşılaştırılmasıdır. AH Plus, AH 26, Endomethasone N, RSA ve Sealapex üreticilerinin talimatları doğrultusunda steril şartlarda karıştırılarak önceden hazırlanan 2 mm çapında ve 10 mm uzunluğundaki standart steril cam kalıplara yerleştirildi. Test örnekleri, taze, 3. gün ve 7. günde değerlendirilmek üzere 3 gruba ayrıldı. Farklı sertleşme zamanına sahip örnekler, 1 günlük, 4 günlük ve 7 günlük ekstraksiyon periyotları için 10 ml’lik kültür ortamda dilüe edildiler. Her dilüsyon periyodundan sonra ekstre alındı, 0.22 µm lik filtrede steril edildi ve 1:1, 1:2, 1:4, 1:8 oranlarında seyreltilerek, 1 günlük inkübasyon süresince 3T3 fare fibroblastlarına uygulandı. Yaşayabilen hücre oranları MTS (tetrazolium salt) analizi yapılarak değerlendirildi. Araştırmamızı oluşturan çeşitli değişkenler, oluşturdukları sitotoksisiteye göre, hem kendi aralarında hem de kendi içlerinde istatistiksel olarak değerlendirildi. Araştırmamızda, her kanal patının, her sertleşme ve ekstraksiyon süresinin ve her seyreltme oranının kendi aralarında istatistiksel olarak önemli farklılıklar gösterdiği bulundu (p AH 26 > Endomethasone N > AH Plus > RSA olarak sıralandı. Ayrıca çalışmada sertleşme zamanı ve seyreltilme oranı artıkça, sitotoksisite azalırken, ekstraksiyon süresi uzadıkça sitotoksisitenin arttığı görüldü

Anahtar Kelimeler

Biyouyumluluk, Kök kanal patları, Sitotoksisite

Kaynakça

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