POLYMERASE CHAIN REACTION (PCR) AND ENDODONTIC MICROBIOLOGY

Yıl 2006, Cilt: 2006 Sayı: 2, 61 - 65, 01.02.2006

Dt. Özgür İlke Atasoy Ulusoy Prof. Dr. Güliz Görgül

Öz

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Anahtar Kelimeler

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POLİMERAZ ZİNCİR REAKSİYONU (PCR) VE ENDODONTİK MİKROBİYOLOJİ

Öz

Bu makale polimeraz zincir reaksiyonunun (Polymerase Chain Reaction-PCR) endodontide kullan m n , endodontik mikroorganizmalar n varl 6 n n ve prevalanslar n n belirlenmesinde sa6lad 6 avantajlargözden geçirmektedir. Kültür yöntemiyle üretilemeyen mikroorganizmalar n 16S rRNA bölgelerinin PCR kullan larak ço6alt lmas moleküler mikrobiyolojide ç 6 raçm t r. PCR kültür yöntemlerine göre daha hassas, özgül, h zl d r ve daha kesin sonuçlar sa6lar. Bu metodlar n yerinde kullan m , endodontik hastal klar nnedenlerinin belirlenmesine, anla lmas na ve tedavi stratejilerinin geli tirilmesine önemli katk lar sa6layacakt r

Anahtar Kelimeler

PCR, virulans, mikroorganizma, kök kanal

Kaynakça

• Sjögren U, Hanström L, Happonen RP, Sundqvist G. Extensive bone loss associated with periapical infection with Bacteroides gingivalis: a case report. Int Endod J 1990 Sep; 23(5): 254-262.
• Oliveira JCM, Siqueira JF, Alves G, Hirata RJ, Andrade AFB. Detection of Porpyromonas endodontalis in infected root canals by 16S rRNA gene-directed polymerase chain reaction. J Endod 2000; 26(12):729-732.
• Griffee MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW. The relationship of Bacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1980; 50: 457-461.
• Sundqvist G, Johansson E, Sjögren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod 1989; 15: 13-19.
• Yoshida M, Fukushima H, Yamamoto K, Ogawa T, Toda T, Sagawa H. Correlation between clinical symptoms and microorganisms isolated from root canals of teeth with periapical pathosis. J Endod 1987;13:24-28.
• Fouad AF, Barry J, Caimano M, Clawson M, Zhu Q, Carver R, Hazlett K, Radolf JD. PCR-based identification of bacteria associated with endodontic infections. J Clin Microbiol 2002; 40(9): 3223- 3231.
• Siqueira JF, Roças IN. PCR methodology as a valuable tool for identification of endodontic pathogens. J Dent 2003; 31(5): 333-339.
• Odell LJ, Baumgartner JG, Xia T, David LL. Survey for collagenase gene prtC in Porphyromonas gingivalis and Porphyromonas endodontalis isolated from endodontic infections. J Endod 1999; 25(8): 555-558.
• McPherson MJ, Moller SG. The Basics. New York: Cromwell Press ; 2000, 1-45.
• Siqueira JF, Roças IN. Simultaneous detection of Dialister pneumosintes and Filifactor alocis in endodontic infections by 16Sr DNA-directed multiplex PCR. J Endod 2004; 30(12): 851-854.
• Siqueira JF, Roças IN. Bacteroides forsytus in primary endodontic infections as detected by nested PCR. J Endod 2003; 29(6): 390-393.
• Xia T, Baumgartner C. Occurence of Actinomyces in infections of endodontic origin. J Endod 2003; 29(9): 549-552.
• Siqueira JF, Roças IN. Treponema socranskii in primary endodontic infections as detected by nested PCR. J Endod 2003; 29(4): 244-247.
• Siqueira JF, Roças IN. Dialister pneumosintes can be a suspected endodontic pathogen. 2002; 94: 494-498
• Siqueira JK, Roças IN. Pseudoramibacter alactolyticus in primary endodontic infections. J Endod 2003; 29(11): 735-738.
• Xia T, Baumgartner JC, David LL. Isolation and identification of Prevotella tannerae from endodontic infections. Oral Microbiol Immunol. 2000 Aug; 15(4): 273-275.
• Bogen G, Slots J. Black-pigmented anaerobic rods in closed periapical lesions. Int Endod J 1999; 32: 204-210.
• Siqueira JF, Roças IN. Polymerase chain reactionbased analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 97: 85-94.
• Sorsa T, Ingman T, Suomalainen K, et al. Identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. Infection and Immunity 1992 Nov; 60(11): 4491-4495.
• Yang LC, Tsai CH, Huang FM, et al. Induction of vascular endothelial growth factor expression in human pulp fibroblasts stimulated with blackpigmented Bacteroides. Int Endod J 2004; 37:588-592.
• Beikler T, Ehmke B, Wittstock M, Schmidt H, Karch H, Flemmig TF.Serum antibody reactivity against recombinant prtC of Porpyromonas gingivalis following periodontal theraphy. J Periodont Res 2003; 38: 276-281.
• Kawada M, Yoshida A, Suzuki N, et al. Prevalence of Porpyromonas gingivalis in relation to periodontal status assessed by real-time PCR. Oral Microbiol Immunol 2004; 19: 289-292.
• Siqueira JF. Jr, Roças IN, Alves FR, Santos KR. Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod. 2004 Sep; 30(9): 638-643.
• Yung IY, Choi B, Kum KY, et al. Identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Sep; 92(3): 329-334.
• Bate AL, Ma JKC, Pitt Ford TR. Detection of bacterial virulence genes associated with infective endocarditis in infected root canals. Int Endod J 2000; 33: 194-203.
• Norris JM, Love DN. The association of two recombinant proteinases of a feline strain of Porpyromonas gingivalis with periodontal disease in cats. Vet Microbiol 2000; 71: 69-80.
• Dupre I, Zanetti S, Schito AM, Fadda G, Sechi LA. Incidence of virulence determinants in clinical Enterococcus faecalis isolates collected in Sardinia (Italy). J Med Microbiol 2003; 52: 491-498.
• Creti R, Imperi M, Bertuccini L, et al. Survey for virulence determinants among Enterococcus faecalis isolated from different sources. J Med Microbiol 2004; 53: 13-20.
• Hong CY, Lin SK, Kok SH, et al. The role of lipopolysaccharide in infectious bone resorption of periapical lesion. J Oral Pathol Med 2004; 33: 162-169.
• Chang YC, Huang FM, Yang SF, et al. Induction of cyclooxygenase-2 mRNA and protein expression in human pulp cells stimulated with blackpigmented bacteroides. J Endod 2003; 29(4):240-243.
• Yang LC, Tsai CH, Huang FM, Liu CM, Lai CC, Chang YC. Induction of interleukin-6 gene expression by pro-inflammatory cytokines and black-pigmented Bacteroides in human pulp cell cultures. Int Endod J 2003; 36: 352-357.
• Yang LC, Huang FM, Lin CS, Liu CM, Lai CC, Chang YC. Induction of interleukin-8 gene expression by black-pigmented Bacteroides in human pulp fibroblasts and osteoblasts. Int Endod J 2003; 36: 774-779.
• Nakata K, Yamasaki M, Iwata T, Suzuki K, Nakane A, Nakamura H. Anaerobic bacterial extracts influence production of matrix metalloproteinases and their inhibitors by human dental pulp cells. J Endod 2000; 26(7): 410-413.
• Chang YC, Lai CC, Yang SF, Chan Y, Hsieh YS. Stimulation of matrix metalloproteinases by blackpigmented bacteroides in human pulp and periodontal ligament cell cultures. J Endod 2002; 28(2): 90-93.
• Pattamapun K, Tiranathanagul S, Kuwatanasuchat J, Pavasant P. Activation of MMP-2 by Porpyromonas gingivalis in human periodontal ligament cells. J Periodontal Res 2003; 38: 115-121.
• Heling I, Morag-Hezroni M, Marva E, Hochman N, Zakay-Rones Z, Morag A. Is herpes simplex virus associated with pulp/periapical inflammation? Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Mar; 91(3): 359-361.
• Glick M, Trope M, Bagasra O, Pliskin ME. Human immunodeficiency virus infection of fibroblasts of dental pulp in seropositive patients. Oral Surg Oral Med Oral Pathol. 1991 Jun; 71(6): 733-736.
• Elkins DA, Torabinejad M, Schmidt RE, Rossi JJ, Kettering JD. Polymerase chain reaction detection of human immunodeficiency virus DNA in human periradicular lesions. J Endod. 1994 Aug; 20(8):386-388.
• Sabeti M, Slots J. Herpesviral-bacterial coinfection in periapical pathosis. J Endod 2004; 30(2): 69-72.