ÇÜRÜK DİRENCİNİN ARTTIRILMASINDA LAZER VE KAZEİN FOSFOPEPTİD AMORF KALSİYUM FOSFAT’IN BİRLİKTE KULLANIMIN ETKİSİNİN DEĞERLENDİRİLMESİ

Yıl 2018, Cilt: 28 Sayı: 1, 23 - 32, 28.03.2018

Şule Bayrak Elif İpek Yılmaz

https://doi.org/10.17567/ataunidfd.410361

Öz

Amaç: Bu
çalışmanın amacı, erbiyumla takviyelenmiş yitriyum alüminyum garnet lazerin
kazein fosfopeptid amorf kalsiyum fosfat ile birlikte kullanımının daimi
dişlerin çürük oluşumuna karşı direncinin arttırılmasındaki etkilerinin
değerlendirilmesidir.

Gereç ve Yöntem: 30 adet daimi diş
kullanılarak elde edilen mine örnekleri rastgele 5 gruba ayrıldı; negatif
kontrol, asitlenmiş fosfat florit (pozitif kontrol), kazein fosfopeptid amorf kalsiyum fosfat, lazer, lazer+kazein fosfopeptid phosphopeptide amorf kalsiyum fosfat.
Gruplara göre yüzey tedavileri uygulandıktan sonra örnekler pH siklusuna tabi
tutuldu. Polarize ışık mikroskopisi ile çürük lezyon derinliği, tarayıcı
elektron mikroskopisi ile yüzeyde oluşan yapısal değişiklikler değerlendirildi.
Elde edilen verilerin istatistiksel
değerlendirilmesinde tek yönlü varyans analizi ve Tukey Çoklu Karşılaştırma testi kullanıldı.

Bulgular: Tüm
gruplar arasında en yüksek ortalama çürük lezyon derinliği negatif kontrol grubunda
(51,12±10,79), en düşük ortalama çürük lezyon derinliği ise asitlenmiş fosfat
florit (pozitif kontrol) grubunda (24,12±11,73) gözlendi. Lazerin tek başına ve
CPP-ACP ile birlikte uygulanması karşılaştırıldığında, gruplar arasında
istatistiksel olarak anlamlı bir farklılık saptanmadı
(p>0,05). Taramalı elektron mikroskobu incelemesinde ise tüm
gruplarda uygulanan yüzey hazırlama işlemlerine göre morfolojik değişimlerin
olduğu gözlendi.

Sonuç: İn vitro
olarak yapılan bu çalışma sonucunda, daimi dişlerin çürük direncinde erbiyumla
takviyelenmiş yitriyum alüminyum garnet lazerin, kazein fosfopeptid amorf kalsiyum fosfat ile birlikte kullanımın
tek başına kullanıma göre ilave bir katkı sağlamadığı belirlendi.

Anahtar
Kelimeler: Diş çürüğüne direnç, diş minesi, kazein fosfopeptid amorf kalsiyum
fosfat, lazer

EVALUATION OF COMBINED EFFECT OF LASER AND CASEIN
PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE APPLICATION ON INCREASING OF CARIES
RESISTANCE

Aim: The aim of this study was to
evaluate the effects of erbium-doped yttrium aluminum garnet laser combined
with casein
phosphopeptide-amorphous calcium phosphate treatments
on caries resistance of permanent teeth

Material and Methods: The enamel samples were obtained from 30 permanent teeth and randomly
divided into five groups; negative control, acidulated phosphate fluoride
(positive control), casein phosphorpeptide-amorphous calcium phosphate, laser,
laser+ casein phosphopeptide-amorphous calcium phosphate. The surface
treatments were applied; the samples were then submitted to pH cycle. Lesion
depth was measured by polarized light microscopy, and the morphological changes
on the sample surfaces were analyzed by scanning electron microscopy. The statistical analyses of obtained data were performed
using one-way analysis of variance and Tukey Multiple Comparison test.

Results: Mean lesion depth was
greatest in the nega- tive control group
(51.12±10.79), and lowest in the acidulated phosphate fluoride group fluoride (positive control)
(24.12±11.73). When compared the laser group and laser combined with casein phosphopeptide-amorphous calcium phosphate, no
statistically sig- nificant differences in mean lesion depth values were found
between groups (p>0.05). Scanning electron microscopy examination also
revealed obvious morphological changes in all groups.

Conclusion: As a result of this in-vitro study determined that
the combined application of aluminum erbium- doped yttrium garnet laser with
casein phosphorpeptide-amorphous calcium phosphate no showed an added
beneficial effect compared with erbium-doped yttrium aluminum garnet laser
alone on caries resistance of permanent teeth.

Keywords: Dental caries resistance,
dental enamel, casein
phosphopeptide-amorphous calcium phosphate, laser.

Anahtar Kelimeler

Diş çürüğüne direnç, diş minesi, kazein fosfopeptid amorf kalsiyum fosfat

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