NİSTATİN VE TİTANYUM DİOKSİT İLAVESİNİN YUMUŞAK ASTAR MATERYALİNDEKİ CANDIDA KOLONİZASYONU ÜZERİNE ETKİSİNİN KARŞILAŞTIRILMASI

Yıl 2018, Cilt: 28 Sayı: 1, 48 - 54, 28.03.2018

Funda Bayındır Esra Kul , Ayşe Esin Aktaş Ayşe :baştopçu

https://doi.org/10.17567/ataunidfd.410379

Öz

Amaç: Tam protez
kullanan hastalarda, yumuşak astar materyalleri, mukozayı irrite eden lezyon
oluşumları ile sonuçlanan, Candida
albicans’ ların adezyonunu
COMPARISON OF THE EFFECT OF NYSTATIN AND TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL






ABSTRACT
ve kolonizasyonunu destekleyebilir. Bu
çalışmanın amacı, nistatin ve titanium dioksit [TiO₂]
antifungal ajanlarının, yumuşak astar maddelerindeki
Candida albicans kolonizasyonunu
önlemedeki etkisini değerlendirmektir.

Gereç ve Yöntem: Ufi Gel P
ve Mollosil yumuşak astar materyallerinden 5mm çapında ve 2mm kalınlığında, 5%, 10%, and 15% konsantrasyonda
nistatin ve titanyum dioksit içeren diskler
hazırlandı. Kontrol grubu için ise nistatin ve TiO₂
içermeyen, sadece yumuşak astar maddesinden oluşan diskler hazırlandı. C.
albicans’ lar Sabouraud
Dekstroz Agar (SDA) kaplı besiyerine inoküle edildi. Hazırlanan diskler
besiyerlerine yerleştirildi. 37° C’de inkübe
edildi.

Çalışmanın
ikinci basamağı için yeniden hazırlanan 10% w/v nistatin içeren diksler  zamanla antifungal etkinin değerlendirilmesi
için farklı süreler (1, 7, 14, and 16 gün) boyunca su içinde bekletildiler.
Daha sonra sudan çıkarılıp, C. albicans
inoküle edilmiş SDA plağına yerleştirildiler. Her gruba nistatin veya TiO₂
içermeyen bir kontrol diski yerleştirildi.

Bulgular: Uygulanan varyans analizi sonucu
kontrol disklerinde istatistiksel olarak anlamlı bir C. albicans inhibisyonu yoktu (p
<0.001). İn vitro olarak nistatin’in toz formunun yumuşak astarlara
eklenmesi, C. albicans kolonizasyonunu
önledi. Disklerin suda bekleme süresi, inhibisyon derecesini etkiledi. Fakat,
yumuşak astarlara TiO₂
ilavesi, C. albicans kolonizasyonunu
önlemedi.

Sonuç: Bu
çalışmanın sonucuna göre, in vitro olarak, nistatinin toz formunun yumuşak
astarlara ilave edilmesi, C. albicans kolonizasyonunu önledi fakat aynı miktardaki TiO₂
tozu eklenmesi
etki göstermedi.

Anahtar Kelimeler: Nistatin,
titanyum dioksit, Candida albicans

COMPARISON OF THE EFFECT OF NYSTATIN AND
TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL

ABSTRACT

Aim: Soft liners can support the adhesion and colonization
of Candida albicans, which can then
irritate the mucosa of denture wearers, resulting in the formation of lesions.The
goal of this study was to evaluate the efficacy of fungicidal agents, nystatin
and titanium dioxide [TiO₂], in preventing the colonization by Candida albicans on soft lining
materials.

Material and methods: Disks of Ufi Gel P and Mollosil cold-curing, soft
relining materials with a diameter of 5 mm and thickness of 2 mm were prepared
with nystatin and TiO₂ powders in a concentration of 5%, 10%, and
15% (w/v). For the control group, only discs not containing nystatin and TiO₂
were prepared, which consisted only of soft lining material. C. albicans was inoculated on Sabouraud
dextrose agar (SDA)-coated plates. The disks were placed on these plates and
incubated aerobically at 37° C.

The Ufi Gel P and Mollosil disks, including 10% w/v
nystatin, (re-prepared for the second step of the study) were immersed in water
for separate intervals (1, 7, 14, and 16 days) to determine the fungicidal action
over time. The disks were then removed and placed on SDA plates inoculated with
C. albicans. In each group, one
control disk without nystatin or TiO₂ was included.

Results: A univariate analysis of variance test revealed that the activity of C. albicans was not significantly
inhibited on the control disks (p <
0.001). The addition of nystatin in powder form to the soft liners prevented
the colonization of C. albicans in vitro. The immersion time of the
disks plunged in water appeared to affect the degree of inhibition. However, the addition of TiO₂ did
not prevent colonization by C. albicans
of the soft liners.

Conclusion: This study showed that the addition of nystatin in powder form to soft
liners prevented the colonization of C.
albicans in vitro but that the
addition of the same amount of TiO₂ powder did not.

Keywords: Nystatin,
titanium dioxide, Candida albicans

Anahtar Kelimeler

Nistatin, titanyum dioksit

Kaynakça

• 1. Nikawa H, Iwanaga H, Kameda M, Hamada T. In vitro evaluation of Candida albicans adherence to soft denture-lining materials. J Prosthet Dent 1992;68:804-8.
• 2. Razek MK, Mohamed ZM. Influence of tissue-conditioning materials on the oral bacteriologic status of complete denture wearers. J Prosthet Dent 1980;44:137-42.
• 3. Arendorf TM Walker DM. Denture stomatitis: a review. J Oral Rehabil 1987;14:217-27.
• 4. Vural C, Ozdemir G, Kurtulmus H, Kumbuloglu O, Ozcan M. Comparative effects of two different artificial body fluids on Candida albicans adhesion to soft lining materials. Dent Mater J 2010;29:206-12.
• 5. Budtz-Jørgensen E, Theilade E, Theilade J. Quantitive relationship between yeasts and bacteria in denture-induced stomatitis. Scand J Dent Res 1983;91:134-42.
• 6. Truhlar MR, Shay K, Sohnle P. Use of a new assay technique for quantification of antifungal activity of nystatin incorporated in denture liners. J Prosthet Dent 1994;71:517-24.
• 7. Budtz-Jorgensen E, Bertram U. Denture stomatitis. I. The etiology in relation to trauma and infection. Acta Odontol Scand 1970;28:71-92.
• 8. Alcantara CS, Macedo AF, Gurgel BC, Jorge JH, Neppelenbroek KH, Urban VM. Peel bond strength of resilient liner modified by the addition of antimicrobial agents to denture base acrylic resin. J Appl Oral Sci 2012;20:607-12.
• 9. Banting DW, Greenhorn PA, McMinn JG. Effectiveness of a topical antifungal regimen for the treatment of oral candidiasis in older, chronically ill, institutionalized, adults. J Can Dent Assoc 1995;61:199-200, 203-5.
• 10. Arendorf TM, Walker DM. Oral candidal populations in health and disease. Br Dent J 1979;147:267-72.
• 11. Torres SR, Peixoto CB, Caldas DM, Silva EB, Akiti T, Nucci M, et al. Relationship between salivary flow rates and Candida counts in subjects with xerostomia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:149-54.
• 12. Radnai M, Whiley R, Friel T, Wright PS. Effect of antifungal gels incorporated into a tissue conditioning material on the growth of Candida albicans. Gerodontology 2010;27:292-6.
• 13. Williamson RT. Clinical application of a soft denture liner: a case report. Quintessence Int 1995;26:413-8.
• 14. Pavan S, dos Santos PH, Filho JN, Spolidorio DM. Colonisation of soft lining materials by micro-organisms. Gerodontology 2010;27:211-6.
• 15. Braden M, Wright PS, Parker S. Soft lining materials--a review. Eur J Prosthodont Restor Dent 1995;3:163-74.
• 16. Qudah S, Harrison A, Huggett R. Soft lining materials in prosthetic dentistry: a review. Int J Prosthodont 1990;3:477-83.
• 17. Murata H, Taguchi N, Hamada T, Kawamura M, McCabe JF. Dynamic viscoelasticity of soft liners and masticatory function. J Dent Res 2002;81:123-8.
• 18. Murata H, Hong G, Li YA, Hamada T. Compatibility of tissue conditioners and dental stones: effect on surface roughness. J Prosthet Dent 2005;93:274-81. 19. Chow CK, Matear DW, Lawrence HP. Efficacy of antifungal agents in tissue conditioners in treating candidiasis. Gerodontology 1999;16:110-8.
• 20. Geerts GA, Stuhlinger ME, Basson NJ. Effect of an antifungal denture liner on the saliva yeast count in patients with denture stomatitis: a pilot study. J Oral Rehabil 2008;35:664-9.
• 21. Douglas WH, Walker DM. Nystatin in denture liners – an alternative treatment of denture stomatitis. Br Dent J1973;135:55-9.
• 22. Skupien JA, Valentini F, Boscato N, Pereira-Cenci T. Prevention and treatment of Candida colonization on denture liners: a systematic review. J Prosthet Dent 2013;110:356-62.
• 23. Song R, Jiao X, Lin L. Improvement of mechanical and antimicrobial properties of denture base resin by nano-titanium dioxide and nanosilicon dioxide particles. Pigment and Resin Technology 2011;40:393-8.
• 24. Uchimaru M, Sakai T, Moroi R, Shiota S, Shibata Y, Deguchi M, Sakai H, Yamashita Y, Terada Y. Antimicrobial and antifungal effects of tissue conditioners containing a photocatalyst. Dent Mater J 2011;30:691-9.
• 25. Urban VM, de Souza RF, Arrais CA, Borsato KT, Vaz LG. Effect of the association of nystatin with a tissue conditioner on its ultimate tensile strength. J Prosthodont 2006;15:295-9.
• 26. Patel MP, Cruchley AT, Coleman DC, Swai H, Braden M, Williams DM. A polymeric system for the intra-oral delivery of an anti-fungal agent. Biomaterials 2001;22:2319-24.
• 27. Falah-Tafti A, Jafari AA, Lotfi-Kamran MH, Fallahzadeh H, Hayan RS. A comparison of the efficacy of nystatin and fluconazole incorporated into tissue conditioner on the in vitro attachment and colonization of Candida albicans. Dent Res J 2010;7:18-22.
• 28. Quinn DM. The effectiveness, in vitro, of miconazole and ketoconazole combined with tissue conditioners in inhibiting the growth of Candida albicans. J Oral Rehabil 1985;12:177-82.
• 29. Bal BT, Yavuzyılmaz H. Yumuşak astar maddeleri. Atatürk Üniv Diş Hek Fak Derg 2006;Suppl 1:53-60.
• 30. Gruber RG, Lucatarto EM, Molnar EJ. Fungus growth on tissue conditioners and soft denture liners. Rev Dent Liban 1968;18:36-43.
• 31. Carter GM, Kerr MA, Shepherd MG. The rational management of oral candidosis associated with dentures. N Z Dent J 1986;82:81-4.
• 32. Schneid TR. An in vitro analysis of a sustained release system for the treatment of denture stomatitis. Spec Care Dentist 1992;12:245-50.
• 33. Montagner H, Montagner F, Braun KO, Peres PE, Gomes BP. In vitro antifungal action of different substances over microwaved-cured acrylic resins. J Appl Oral Sci 2009;17:432-5.
• 34. Aldana L, Marker VA, Kolstad R, Iacopino AM. Effects of Candida treatment regimens on the physical properties of denture resins. Int J Prosthodont 1994;7:473-8.
• 35. Martínez-Beneyto Y L-JP, Velandrino-Nicolás A, Jornet-García V. Use of antifungal agents for oral candidiasis: results of a national survey. Int J Dent Hyg 2010;8:47-52.
• 36. Sivakumar I, Arunachalam KS, Sajjan S, Ramaraju AV, Rao B, Kamaraj B. Incorporation of antimicrobial macromolecules in acrylic denture base resins: a research composition and update. J Prosthodont 2014;23:284-90.
• 37. Sawada T, Sawada T, Kumasaka T, Hamada N, Shibata T, Nonami T, Kimoto K. Self-cleaning effects of acrylic resin containing fluoridated apatite-coated titanium dioxide. Gerodontology 2014;31:68-75.
• 38. Akiba N, Hayakawa I, Keh ES, Watanabe A. Antifungal effects of a tissue conditioner coating agent with TiO2 photocatalyst. J Med Dent Sci 2005;52:223-7.