ALTERNATİF BİR İRİGASYON SOLUSYONU OLARAK MELATONİNİN SMEAR TABAKASI UZAKLAŞTIRMA ETKİNLİĞİNİN İNCELENMESİ: SEM ÇALIŞMASI

Yıl 2019, Cilt: 29 Sayı: 4, 576 - 581, 15.10.2019

Emrah Karataşlıoğlu Samet Tosun

https://doi.org/10.17567/ataunidfd.603731

Öz

Amaç: Bu çalışmanın
amacı, melatoninin kök kanal irigasyon solüsyonu olarak tek başına, % 5.25
sodyum hipoklorit ve % 17 etilendiamintetraasetikasit ile
birlikte kullanımının smear tabakasını kaldırma etkisini incelemektir.

Gereç ve Yöntem: Toplam 75 adet
yeni çekilmiş, tek köklü, insan maksiller santral kesici dişi bu çalışma için
seçildi. Dişler kuronlarından keserek ayrıldıktan sonra kalan kökler irigasyon
protokollerine göre (n = 15) beş gruba ayrıldı: Grup 1: % 5.25 sodyum
hipoklorit; Grup 2: % 0.02 melatonin Grup 3: % 5.25 sodyum hipoklorit + % 17
etilendiamintetraasetikasit; Grup 3: % 5.25 sodyum hipoklorit + % 0.02 melatonin
Grup 5: % 0.02 melatonin + % 17 etilendiamintetraasetikasit. Kökler uzun
eksenleri boyunca iki yarım parçaya ayrıldı. Bu parçalar üzerinde smear
tabakasının varlığı; şayet var ise miktarı taramalı elektron mikroskobu
kullanılarak incelendi ve skorlandı. Elde edilen veriler SPSS programında
Kruskal-Wallis testi kullanılarak istatistiksel olarak analiz edildi (α =0.05).

Bulgular: Melatonin, sodyum hipoklorit ile
kıyaslandığında kök kanalının koronal ve orta üçlü bölgesinde smear kaldırma
kapasitesi anlamlı derecede yüksek bulundu (p<0.05). Sodyum hipoklorit + etilendiamintetraasetikasit
kullanımı ile melatonin + etilendiamintetraasetikasit kullanımı koronal üçlüde
smear kaldırma etkisi açısından anlamlı bir fark göstermedi (p>0.05). Apikal
bölgede grupların smear kaldırma etkinlikleri değerlendirildiğinde sodyum
hipoklorit + etilendiamintetraasetikasit en iyi smear kaldırma kapasitesine
sahip bulundu. (p<0.05)

Sonuç: Bu çalışmanın sınırları dahilinde, 0.2
mg / mL melatonin 1 dakika boyunca irigant olarak kullanımı smear tabakasının
kaldırılmasında anlamlı derecede etkili bulunmuştur. Melatoninin konsantrasyonunun,
uygulama süresinin veya miktarının smear tabakasını kaldırma kapasitesini nasıl
etkileyeceğini belirlemek için daha fazla araştırmaya ihtiyaç vardır.

Anahtar Kelimeler: Melatonin,
Smear, Taramalı Elektron Mikroskobu.

 

INVESTIGATION OF SMEAR LAYER REMOVAL EFFECTIVENESS OF
MELATONIN AS AN ALTERNATIVE IRRIGATION SOLUTION: SEM STUDY

Abstract

Aim: The
aim of this study was to investigate the smear layer removal effectiveness of
melatonin which was used alone, together with 5.25% sodium hypochlorite and
with 17% ethylenediaminetetraaceticacid as a root canal irrigation solution.

Materials and Methods: A
total of 75 freshly-extracted, single-rooted, human maxillary central incisors
were selected for this study. Following decoronation of teeth, remaining root
parts were subdivided into five groups according to
irrigation protocols
groups (n = 15): Group
1: 5.25 % sodium hypochlorite; Group 2: 0.02 % melatonin; Group 3: 5.25
% sodium hypochlorite + 17 % ethylenediaminetetraaceticacid; Grup 4: 5.25 % sodium
hypochlorite + 0.02 % melatonin; Grup 5: 0.02 % melatonin + 17 % ethylenediaminetetraaceticacid. All the roots were
longitudinally split into two halves. Presence of smear layer, if present the
amount of it was examined and scored on these half parts by using scanning
electron microscopy. The data were analyzed statistically by using
Kruskal-Wallis test in SPSS program (α = 0.05).

Results: When melatonin
was compared to sodium hypochlorite, smear removal capacity was significantly
higher in coronal and middle one–third region of the root canal (p <0.05). No
significant difference was observed between the use of sodium hypochlorite + ethylenediaminetetraaceticacid and melatonin + ethylenediaminetetraaceticacid in
terms of smear removal capacity on coronal one–third region (p> 0.05). When
smear removal effect of the groups were evaluated, sodium hypochlorite + ethylenediaminetetraaceticacid
was found to have the best smear removal capacity in the apical one-third
region (P <0.05).

Conclusions: Within the
limits of this study, the use of 0.2 mg / mL melatonin as an irigant for 1
minutes application was found to be significantly effective in removing the
smear layer. Further research is needed to determine how the concentration,
amount or duration of melatonin will affect the capacity to remove the smear
layer.

Key word: melatonin,
smear, scanning electron microscopy.

Anahtar Kelimeler

Melatonin, Smear

Kaynakça

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