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CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS

Yıl 2022, Cilt: 32 Sayı: 1, 34 - 37, 15.02.2022
https://doi.org/10.17567/ataunidfd.1030475

Öz

Aim: The aim of this study was to observe the cytotoxic effect of Resin modified Glass Ionomer Cement (RMGIC) on human dental pulp stem cells (DPSCs) proliferation by using xCELLigence®, a device that measures real-time cell viability and evaluates cytotoxic effects, and to determine the CC50 value on these cells for 72 hours.
Materials and Methods: DPSCs obtained from the American Type Culture Collection were seeded on E-Plates. After 24 hours, three different dilutions (100%, 10% and 1%) of the elution obtained from RMGIC were added to three wells. DMEM solution was used as the control group. Real time cell index data were acquired by using xCELLigence® device for 72 hours. In order to compare cell index values, repeated-measures analysis of variance and linear regression analysis were used.
Results: In contrast to the 100% dilution of RMGIC which exhibited toxic effect on DPSCs, its 1% dilution showed proliferative effect. And 10% dilution was similar to the control group. While the coefficient of determination was above 80% in all groups, it was found to be lower only in the RMGIC 100%-dilution group by 0.7%. Also, the regression coefficient was found to be significantly different from zero in all equations except RMGIC 100%-dilution group (p<0.001). CC50 values of RMGIC at the 24th, 48th and 72nd hours were 5.07%, 5.07% and 5.08%, respectively.
Conclusion: In order to provide more reliable results, CC50 values determined in our study will guide the further studies to improve RMGICs by adding different molecules to its structure for reducing its cytotoxicity.
Keywords: Glass ionomer cements, dental pulp stem cell, mesenchymal stem cell, cytotoxicity, xCELLigence

Rezin ile Modifiye Edilmiş Cam İyonomer Simanların Diş Pulpası Kök Hücreleri Üzerindeki Sitotoksisitesi


Kaynakça

  • 1. Wilson AD and Kent BE. A new translucent cement for dentistry. The glass ionomer cement. Br Dent J. 1972; 132(4): 133-5.
  • 2. Mathis RS and Ferracane JL. Properties of a glass-ionomer/resin-composite hybrid material. Dent Mater. 1989; 5(5): 355-8.
  • 3. Sidhu SK and Schmalz G. The biocompatibility of glass-ionomer cement materials. A status report for the American Journal of Dentistry. Am J Dent. 2001; 14(6): 387-96.
  • 4. Ersahan S, Oktay EA, Sabuncuoglu FA, Karaoglanoglu S, Aydin N, and Suloglu AK. Evaluation of the cytotoxicity of contemporary glass-ionomer cements on mouse fibroblasts and human dental pulp cells. Eur Arch Paediatr Dent. 2020; 21(3): 321-8.
  • 5. Nicholson JW and Czarnecka B. The biocompatibility of resin-modified glass-ionomer cements for dentistry. Dent Mater. 2008; 24(12): 1702-8.
  • 6. Volk J, Engelmann J, Leyhausen G, and Geurtsen W. Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts. Dent Mater. 2006; 22(6): 499-505.
  • 7. Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, Wang RS, and Jeng JH. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials. 2005; 26(7): 745-53.
  • 8. Lee DH, Kim NR, Lim BS, Lee YK, and Yang HC. Effects of TEGDMA and HEMA on the expression of COX-2 and iNOS in cultured murine macrophage cells. Dent Mater. 2009; 25(2): 240-6.
  • 9. Mantellini MG, Botero T, Yaman P, Dennison JB, Hanks CT, and Nor JE. Adhesive resin and the hydrophilic monomer HEMA induce VEGF expression on dental pulp cells and macrophages. Dent Mater. 2006; 22(5): 434-40.
  • 10. Schweikl H, Schmalz G, and Rackebrandt K. The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res. 1998; 415(1-2): 119-30.
  • 11. Schweikl H, Schmalz G, and Spruss T. The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res. 2001; 80(7): 1615-20.
  • 12. Smith DC and Ruse ND. Acidity of glass ionomer cements during setting and its relation to pulp sensitivity. J Am Dent Assoc. 1986; 112(5): 654-7.
  • 13. Stanislawski L, Daniau X, Lauti A, and Goldberg M. Factors responsible for pulp cell cytotoxicity induced by resin-modified glass ionomer cements. J Biomed Mater Res. 1999; 48(3): 277-88.
  • 14. Kanjevac T, Milovanovic M, Volarevic V, Lukic ML, Arsenijevic N, Markovic D, Zdravkovic N, Tesic Z, and Lukic A. Cytotoxic effects of glass ionomer cements on human dental pulp stem cells correlate with fluoride release. Med Chem. 2012; 8(1): 40-5.
  • 15. Aranha AM, Giro EM, Souza PP, Hebling J, and de Souza Costa CA. Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line. Dent Mater. 2006; 22(9): 864-9.
  • 16. de Castilho AR, Duque C, Negrini Tde C, Sacono NT, de Paula AB, Sacramento PA, de Souza Costa CA, Spolidorio DM, and Puppin-Rontani RM. Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate. Arch Oral Biol. 2012; 57(2): 131-8.
  • 17. de Castilho AR, Duque C, Negrini Tde C, Sacono NT, de Paula AB, de Souza Costa CA, Spolidorio DM, and Puppin-Rontani RM. In vitro and in vivo investigation of the biological and mechanical behaviour of resin-modified glass-ionomer cement containing chlorhexidine. J Dent. 2013; 41(2): 155-63.
  • 18. Sengun A, Botsalı H, Yalcın M, Ozer F, Tasdemır S, and Hakkı S. Evaluation of cytotoxicity of glass ionomer cements by dentin barrier test. dental materials. 2009; 5(25): e39.
  • 19. Pritchett J, Naesens L, and Montoya J. Treating HHV-6 infections—the laboratory efficacy and clinical use of anti-HHV-6 agents, p 311–331. Human herpesviruses HHV-6A, HHV-6B & HHV-7, 3rd ed. Elsevier, Amsterdam, Netherlands. 2014.
  • 20. ISO 10993-12. Biological Evaluation of Medical Devices - Part 12: Sample Preparation and Reference Materials. International Organization for Standardization 2004.
  • 21. Lan WH, Lan WC, Wang TM, Lee YL, Tseng WY, Lin CP, Jeng JH, and Chang MC. Cytotoxicity of conventional and modified glass ionomer cements. Oper Dent. 2003; 28(3): 251-9.
  • 22. Sasanaluckit P, Albustany KR, Doherty PJ, and Williams DF. Biocompatibility of glass ionomer cements. Biomaterials. 1993; 14(12): 906-16.
  • 23. Schmalz G. The biocompatibility of non-amalgam dental filling materials. Eur J Oral Sci. 1998; 106(2 Pt 2): 696-706.
  • 24. Schmalz G, Thonemann B, Riedel M, and Elderton RJ. Biological and clinical investigations of a glass ionomer base material. Dent Mater. 1994; 10(5): 304-13.
  • 25. Bayindir ZY and Yildiz M. Rezin modifite cam-ionomer simanlar ve poliasit-modifiye kompozit rezinler (kompomer). Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2000(1).
  • 26. Golkar P, Omrani LR, Zohourinia S, Ahmadi E, and Asadian F. Cytotoxic Effect of Addition of Different Concentrations of Nanohydroxyapatite to Resin Modified and Conventional Glass Ionomer Cements on L929 Murine Fibroblasts. Frontiers in Dentistry. 2021.
  • 27. de Souza Costa CA, Hebling J, Garcia-Godoy F, and Hanks CT. In vitro cytotoxicity of five glass-ionomer cements. Biomaterials. 2003; 24(21): 3853-8.
  • 28. Ahmed HM, Omar NS, Luddin N, Saini R, and Saini D. Cytotoxicity evaluation of a new fast set highly viscous conventional glass ionomer cement with L929 fibroblast cell line. J Conserv Dent. 2011; 14(4): 406-8.
  • 29. Collado-Gonzalez M, Pecci-Lloret MR, Tomas-Catala CJ, Garcia-Bernal D, Onate-Sanchez RE, Llena C, Forner L, Rosa V, and Rodriguez-Lozano FJ. Thermo-setting glass ionomer cements promote variable biological responses of human dental pulp stem cells. Dent Mater. 2018; 34(6): 932-43.
  • 30. Botsali MS, Kusgoz A, Altintas SH, Ulker HE, Tanriver M, Kilic S, Basak F, and Ulker M. Residual HEMA and TEGDMA release and cytotoxicity evaluation of resin-modified glass ionomer cement and compomers cured with different light sources. ScientificWorldJournal. 2014; 2014: 218295.
  • 31. Ke N, Wang X, Xu X, and Abassi YA. The xCELLigence system for real-time and label-free monitoring of cell viability. Methods Mol Biol. 2011; 740: 33-43.
  • 32. Demirci T, Gürbüz T, and Şengül F. Dental rezin kompozitlerin sitotoksisitesi: Bir in vitro. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2014; 24(1): 10-5.
  • 33. Souza PP, Aranha AM, Hebling J, Giro EM, and Costa CA. In vitro cytotoxicity and in vivo biocompatibility of contemporary resin-modified glass-ionomer cements. Dent Mater. 2006; 22(9): 838-44.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Diş Hekimliği
Bölüm Araştırma Makalesi
Yazarlar

Şeyma Keskin Bu kişi benim 0000-0003-4861-678X

Fatih Şengül Bu kişi benim 0000-0001-6087-148X

Yayımlanma Tarihi 15 Şubat 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 32 Sayı: 1

Kaynak Göster

APA Keskin, Ş., & Şengül, F. (2022). CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 32(1), 34-37. https://doi.org/10.17567/ataunidfd.1030475
AMA Keskin Ş, Şengül F. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Ata Diş Hek Fak Derg. Şubat 2022;32(1):34-37. doi:10.17567/ataunidfd.1030475
Chicago Keskin, Şeyma, ve Fatih Şengül. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32, sy. 1 (Şubat 2022): 34-37. https://doi.org/10.17567/ataunidfd.1030475.
EndNote Keskin Ş, Şengül F (01 Şubat 2022) CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32 1 34–37.
IEEE Ş. Keskin ve F. Şengül, “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”, Ata Diş Hek Fak Derg, c. 32, sy. 1, ss. 34–37, 2022, doi: 10.17567/ataunidfd.1030475.
ISNAD Keskin, Şeyma - Şengül, Fatih. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32/1 (Şubat 2022), 34-37. https://doi.org/10.17567/ataunidfd.1030475.
JAMA Keskin Ş, Şengül F. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Ata Diş Hek Fak Derg. 2022;32:34–37.
MLA Keskin, Şeyma ve Fatih Şengül. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 32, sy. 1, 2022, ss. 34-37, doi:10.17567/ataunidfd.1030475.
Vancouver Keskin Ş, Şengül F. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Ata Diş Hek Fak Derg. 2022;32(1):34-7.

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