GLUTATHIONE-S-TRANSFERASE GENE POLYMORPHISM AS A RISK FACTOR IN CHILDREN WITH BRONCHIAL ASTHMA

Öz

Aim: This study aimed to investigate whether glutathione-S-transferase gene polymorphism is a risk factor in children with bronchial asthma and whether it could serve as a marker for diagnosis. Materials and Methods: Our study was conducted between May 2004 and May 2005 on patients diagnosed with asthma who presented with respiratory symptoms at the Pediatrics Clinic. The patients were staged according to the GINA criteria and were compared based on age, gender, age of symptom onset, passive smoking, hospital admissions, and atopy. All patients underwent hemogram, sedimentation, PPD, chest X-ray, sinus X-ray, nasal eosinophilia, IgG, A, M, E, skin, sweat, and pulmonary function tests. For the analysis of GSTM1 and GSTT1 genes, the multiplex Polymerase Chain Reaction (PCR) method was used; for the study of Cytochrome P4501A1 gene Msp1 polymorphism, the PCR-Restriction Fragment Length Polymorphism (RFLP) method was employed. Results: Our study was conducted with 105 patients aged 1-15 years and a control group of 30 individuals. There were 38 (36.2%) patients with intermittent asthma, 28 (26.7%) with mild persistent asthma, 28 (26.7%) with moderate persistent asthma, and 11 (10.5%) with severe persistent asthma. The average age was 7.36±2.9, with a gender distribution of 51 females and 54 males. The average age of symptom onset was 3.95±3.1 years. A significant relationship was found between disease severity and the prevalence of atopy (p<0.05). No significant difference was found in gene polymorphism between the asthma patients and the control group (p>0.05). When comparing the mild and severe asthma groups for glutathione-S-transferase gene polymorphism, a statistically significant difference was found in GSTM1 carrier status (p<0.05). Conclusion: Although it is still difficult to fully explain the exact role of GST genes in the pathogenesis of bronchial asthma, the results obtained suggest that there is a relationship between the severity of asthma and the presence of GSTM1 gene deletion.

Anahtar Kelimeler

• Asthma
• Children
• Glutathione transferase

Etik Beyan

There is no conflict of interest.

BRONŞİYAL ASTIMLI ÇOCUKLARDA RİSK FAKTÖRÜ OLARAK GLUTATYON-S-TRANSFERAZ GEN POLİMORFİZMİ

Öz

Amaç: Bu çalışmada bronşiyal astımlı çocuklarda glutatyon-s-transferaz gen polirnorfizminin bir risk faktörü olup olmadığını ve tanı için bir marker olup olamayacağını araştırdık. Gereç ve Yöntemler: Çalışmamız Mayıs 2004-Mayıs 2005 tarihleri arasında Çocuk Sağlığı ve Hastalıkları Polikliniği'ne solunum semptomları ile gelen astım tanısı konulmuş hastalarda gerçekleştirildi. Hastalar GINA kriterlerine göre evrelendirilerek kendi aralarında yaş, cinsiyet, semptomların başlangıç yaşı, pasif sigara içiciliği, hastane yatışı ve atopi yönünden karşılaştırıldı. Bütün hastalara hemogram, sedimentasyon, ppd, akciğer grafisi, sinüs grafisi, nazal eosinofili, Ig G, A, M, E, deri testleri, ter testleri, solunum fonksiyon testleri yapıldı. GST-mü-1 ve GST-tehta1 genlerinin analizi için multipleks Polymerase Chain Reaction(PCR) yöntemi; Sitokrom P 4501A1 geni Mspl polimorfiziminin analizi için PCR-Restriction Fragment Length Polymorphism (RFLP) yöntemi kullanıldı. Bulgular: Çalışmamız yaşları 1-15 yaş arasında değişen 105 hasta ve 30 kontrol grubu ile gerçekleştirildi. İntermittan 38 (%36,2), hafif persistan 28 (%26,7), orta persistan 28 (%26,7), ağır persistan 11 (%10,5) astımlı hasta vardı. Yaş ortalaması 7,36±2,9; cinsiyet dağılımı kız/erkek: 51/54; semptomların başlangıç yaşı ortalama 3,95±3,1 yıldı. Hastalığın şiddeti ile atopi görülme oranı arasında anlamlı bir ilişki saptandı (p<0,05). Astım hastaları ile kontrol gurubu arasında gen polimorfizmi açısından anlamlı fark tespit edilmedi (p>0,05). Hafif ve ağır astım grupları kendi aralarında glutatyon-s-transferaz gen polimorfizmi açısından karşılaştırıldıklarında GSTM 1 taşıyıcılığı açısından istatistiksel olarak anlamlı bir farklılık mevcuttu (p<0,05). Sonuç: GST genlerinin bronşiyal astım patogenezindeki tam rolünü açıklamak henüz çok zor olsa da elde edilen sonuçlar astımın şiddetiyle GSTM1 gen delesyonu varlığı arasında bir ilişki olduğunu göstermektedir.

Anahtar Kelimeler

• Astım
• Çocuklar
• Glutatyon transferaz

Etik Beyan

Çıkar çatısması yoktur.

Kaynakça

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