DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)

Öz

The water samples collected from six stations of Stream Tersakan which include Havza; Suluova-Celtek, Kanlıdere, Bogazkoy, Karasu, Tersakan-Last and eight stations of River Yesilirmak which include Amasya Bridge, Yassıcal, Durucasu, Tasova, Kumkoy, Carsamba entrance, Carsamba Bridge, Hacıomer in Amasya and Samsun Provinces were investigated for detection of Cryptosporidium oocysts by Loop Mediated Isothermal Amplification (LAMP).

The water samples were regularly collected in every month from the 14 stations in investigated area between 2010 December to 2011 November for LAMP tests.Ten of 14 investigated stations were found contaminated with Cryptosporidium oocyst by LAMP. The pollution with Cryptosporidium oocysts were determined in stations which are Tersakan–Havza; Tersakan-Suluova, Celtek; Tersakan-Kanlıdere; Tersakan-Bogazkoy; Tersakan-Karasu Tersakan-Last, Yesilirmak-Amasya Bridge, Yesilirmak-Yassıcal, Yesilirmak-Durucasu, Yesilirmak-Tasova. The high contamination rate of Cryptosporidium oocysts were found in Tersakan–Suluova, Celtek and Tersakan-Bogazkoy stations in the all investigated stations. However, River Yesilirmak which is last in Samsun more clean than Stream Tersakan which is last in Amasya in terms of the Cryptosporidium oocysts contamination. The reason in excess of the rate of Cryptosporidium contamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water.

Anahtar Kelimeler

• Cryptosporidium,surface waters,Samsun,Amasya

YEŞİLIRMAK NEHRİ ve TERSAKAN ÇAYI'NDAN (SAMSUN-AMASYA) ALINAN YÜZEYSEL SU ÖRNEKLERİNDE Cryptosporidium parvum’un LAMP TEKNİĞİYLE ARAŞTIRILMASI

Öz

Amasya il merkezi ve ilçeleri Suluova, Taşova’dan, Samsun ilçeleri Havza ve Çarşamba’dan geçen Tersakan Çayı ve Yeşilırmak Nehri’nden alınan su örneklerinde Cryptosporidium türleri Loop Mediated Isothermal Amplification (LAMP) yöntemi kullanılarak araştırılmıştır. 2010 Aralık-2011 Kasım döneminde 14 istasyondan her ay düzenli su örnekleri alınarak bu örneklere LAMP  testi uygulanmıştır. On dört istasyonun 10’unda Cryptosporidium ookistlerine LAMP yöntemiyle rastlanılmıştır. Cryptosporidium ookistleriyle kontamineli istasyonlar: Tersakan–Havza; Tersakan-Suluova, Çeltek; Tersakan-Kanlıdere; Tersakan-Boğazköy; Tersakan-Karasu; Tersakan-son; Yeşilırmak-Amasya Köprüsü; Yeşilırmak-Yassıçal; Yeşilırmak-Durucasu; Yeşilırmak-Taşova’dır. Araştırılan tüm istasyonların içinde Tersakan–Suluova, Celtek ve Tersakan-Boğazköy istasyonları Cryptosporidium ookistleri açısından en kirli istasyonlar olarak belirlenmiştir. Bununla beraber Amasya ve Samsun’dan geçen Yeşilırmak Nehri’nin Tersakan Çayı’na oranla daha temiz olduğu saptanmıştır. Tersakan Çayı’ndaki Cryptosporidium kontaminasyonun fazlalığı, hayvan besiciliğinin Havza and Suluova ilçelerinde yerleşim yerlerine çok yakın mesafelerde yapılmasına; evsel ve tarımsal atık suların hiçbir işleme tabi tutulmadan direk olarak Tersakan Çayı’na deşarj edilmesine bağlanmaktadır.

Anahtar Kelimeler

• Cryptosporidium,yüzey suları,Samsun,Amasya

Kaynakça

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