ATIK SUDAN İZOLE EDİLEN FENOL DEGRADE EDİCİ BAKTERİLERİN ENZİMATİK VE ANTİBİYOTİK DUYARLILIK AKTİVİTELERİ

Öz

Çalışma kapsamında iki farklı atık su arıtma tesisinin çeşitli ünitelerinden örnek alınmış ve bu örneklerin pH ve element içerikleri belirlenmiştir. Özellikleri belirlenen örneklerden fenollü besiyerine inokülasyon yapılmış ve 19 izolat elde edilmiştir. Bu izole edilen 19 izolattan morfolojik olarak farklı olduğu düşünülen 9 izolatın tanımlaması yapılmıştır. İzolatlar arasında yoğun olarak Pseudomonas türleri belirlenmiştir. Tanımlanan bu 9 izolatın atık sulardaki arıtım potansiyellerini değerlendirebilmek için antibiyotik duyarlılık testi gerçekleştirilmiştir. Ayrıca izolatların amilaz ve proteaz enzim üretim aktiviteleri değerlendirilmiştir. İzolatların ofloksasin, azitromisin ve streptomisine duyarlı oldukları belirlenmiştir. Enzim üretim yetenekleri değerlendirildiğinde ise yalnızca 2 izolat zayıf proteaz aktivitesi gösterirken izolatların çoğu amilaz aktivitesi göstermiştir.

Anahtar Kelimeler

• Atık su,antibiyotik,amilaz

ENZYMATIC AND ANTIBIOTIC SUSCEPTIBILITY ACTIVITIES OF PHENOL DEGRADING BACTERIA ISOLATED FROM WASTE WATER

Öz

In the scope of the study, samples were taken from various units of two different wastewater treatment plants and pH and element contents of these samples were determined. Samples were inoculated on phenolic media and totally 19 isolates were obtained. Of these 19 isolates, 9 of them thought to be morphologically different were identified. Pseudomonas species have been identified dominantly among the isolates. An antibiotic susceptibility test was performed to evaluate the treatment potential of these 9 isolates in the wastewater. In addition, amylase and protease enzyme production activities of isolates were evaluated. It has been determined that isolates are susceptible to ofloxacin, azithromycin and streptomycin. When enzyme production capabilities were evaluated, only 2 isolates showed poor protease activity, while most isolates showed amylase activity.

Anahtar Kelimeler

• Wastewater,antibiotics,amylase

Kaynakça

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