Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması

Cihan Darcan; Özge Kaygusuz; Ezgi Aydın

doi:10.18036/aubtdc.372141

TR EN

Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması

Öz

Escherichia coli’de alternatif sigma faktörü olarak adlandırılan rpoS (σ³⁸) asit şoku, açlık stresi, ozmotik stres, oksidatif stres, DNA hasarı ve durağan faza geçiş dahil olmak üzere farklı streslere karşı hücresel cevapta görevli olan genlerin ekspresyonunu kontrol etmektedir. Bu çalışmada Escherichia coli W3110’da rpoS geninin metal ve pH stresi altındaki rolü araştırılmıştır. E. coli BW25113 suşunda mutant olan rpoS geni, P1kc fajı ile yabani tip E. coli W3110’a aktarılmıştır. Yabani tip E. coli W3110 ve rpoS mutant E. coli W3110 suşunda yaşam deneyleri pH 5.5, 7.0 ve 8.0 fosfat tamponunda olmak üzere 3 farklı pH’da plak sayım metodu ile yapılmıştır. 6 farklı metal varlığında (Zn, Ni, Co, Cd, Ag ve Cu) metal stresindeki rolü minimal inhibisyon konsantrasyonu ve petri damlatma yöntemi ile belirlenmiştir. Çalışılan tüm pH değerlerinde rpoS mutant suşlarda duyarlılık gözlenmiştir. Cd metaline karşı rpoS mutantının oldukça önemli bir direnç gösterdiği belirlenirken, diğer metallerde herhangi bir rolü olmadığı tespit edilmiştir. rpoS geninin rolü, genin tamamlanması yapılarak doğrulaması sağlanmıştır. Bu sonuçlar RpoS alternatif sigma faktörünün Cd metali ile ilişkili genlerin kontrolünde ve pH stresi ile ilişkili genlerde rolü olduğunu göstermektedir

Anahtar Kelimeler

Escherichia coli,rpoS,Metal Stresi,Kadmiyum

METALLERE VE pH STRESİNE KARŞI Escherichia coli’de RpoS’NİN ROLÜNÜN ARAŞTIRILMASI

Öz

RpoS (σ38), which acts as an alternative sigma factor in Escherichia coli, controls the expression of genes responsible for cellular responses to different stresses including acidity, starvation, osmotic, oxidative stress, DNA damage, and stationary phase transition. In this study, the role of the rpoS gene in Escherichia coli W3110 under metals and pH stress was researched. The rpoS gene, which is a mutant in the E. coli BW25113 strain, was transferred by transduction with the P1kc phage to wild-type E. coli W3110. Life experiments in wild-type E. coli W3110 and rpoS mutant E. coli W3110 strains were carried out by plaque counting method at 3 different pH, in pH 5.5, 7.0 and 8.0 phosphate buffer. The role of rpoS in the presence of 6 different metals (Zn, Ni, Co, Cd, Ag and Cu) was also determined by the method of dropping petri. Sensitivity at rpoS mutant strains was observed at all pH values studied. It has been determined that the rpoS mutant E. coli against Cd metal shows a very significant resistance, but it has been found that there is no role in other metals. Verification of the role of the rpoS gene has been achieved by genetic complementation. These results show that the RpoS alternative sigma factor plays a role in the control of genes related to the Cd metal and in the genes associated with pH stress.

Anahtar Kelimeler

Escherichia coli,rpoS,Metal Stress,cadmium

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Cihan Darcan ^*
Türkiye

Özge Kaygusuz Bu kişi benim
BİLECİK ŞEYH EDEBALİ ÜNİVERSİTESİ, BİYOTEKNOLOJİ UYGULAMA VE ARAŞTIRMA MERKEZİ
Türkiye

Ezgi Aydın Bu kişi benim
BİLECİK ŞEYH EDEBALİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Türkiye

Yayımlanma Tarihi

17 Ağustos 2018

Gönderilme Tarihi

28 Aralık 2017

Kabul Tarihi

11 Nisan 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 7 Sayı: 2

DOI

https://doi.org/10.18036/aubtdc.372141

IZ

https://izlik.org/JA92EN28ZU

APA

Darcan, C., Kaygusuz, Ö., & Aydın, E. (2018). Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 7(2), 105-121. https://doi.org/10.18036/aubtdc.372141

AMA

1.Darcan C, Kaygusuz Ö, Aydın E. Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2018;7(2):105-121. doi:10.18036/aubtdc.372141

Chicago

Darcan, Cihan, Özge Kaygusuz, ve Ezgi Aydın. 2018. “Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 7 (2): 105-21. https://doi.org/10.18036/aubtdc.372141.

EndNote

Darcan C, Kaygusuz Ö, Aydın E (01 Ağustos 2018) Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 7 2 105–121.

IEEE

[1]C. Darcan, Ö. Kaygusuz, ve E. Aydın, “Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 7, sy 2, ss. 105–121, Ağu. 2018, doi: 10.18036/aubtdc.372141.

ISNAD

Darcan, Cihan - Kaygusuz, Özge - Aydın, Ezgi. “Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 7/2 (01 Ağustos 2018): 105-121. https://doi.org/10.18036/aubtdc.372141.

JAMA

1.Darcan C, Kaygusuz Ö, Aydın E. Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2018;7:105–121.

MLA

Darcan, Cihan, vd. “Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 7, sy 2, Ağustos 2018, ss. 105-21, doi:10.18036/aubtdc.372141.

Vancouver

1.Cihan Darcan, Özge Kaygusuz, Ezgi Aydın. Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 01 Ağustos 2018;7(2):105-21. doi:10.18036/aubtdc.372141

Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması

Öz

Anahtar Kelimeler

METALLERE VE pH STRESİNE KARŞI Escherichia coli’de RpoS’NİN ROLÜNÜN ARAŞTIRILMASI

Öz

Anahtar Kelimeler

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