MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY

Cilt: 2 Sayı: 1 21 Mart 2012
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MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY

Öz

In order to investigate and find out the bacterial community of hot spring waters in Eskişehir,

Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 ºC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteria was examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 ºC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.

Anahtar Kelimeler

Hot spring; Eskişehir; BIOLOG; VITEK; Ribotype.

Kaynakça

  1. Anonymous (2009). Thermal Springs. (http://turkeytravelresource.com/view/253 /thermal-springs--).
  2. Baker, G.C., Gaffar, S., Cowan, D.A. and Suharto, A.R. (2001). Bacterial commu- nity analysis of Indonesian hot springs. FEMS Microbiology Letters 200, 103- 109.
  3. Belkova, N.L., Tazaki, K., Zakharova, J.R. and Parfenova, V.V. (2007). Activity of bacte- ria in water of hot springs from Southern and Central Kamchatskaya geothermal provinces, Kamchatka Peninsula, Russia. Microbiological Research. 162, 99-107.
  4. Bsop I.D.11 (2007). Identification of Moraxella Species and Morphologically Similar Or- ganisms. Issue No: 2, Issue Date: 14.08.07, Issue by: Standards Unit, Evalu- tions and Standards Laboratory 1-13.
  5. Boone, D.R., Castenholz, R.W., Bergey, D.H., Garrity, G.M. and Staley, J.T. (2001). Bergey's Manual of Systematic Bacteriol- ogy, Second Edition, Springer, New York.
  6. Cordevant, C., Tang, J.S., Cleland, D. and Lange, M. (2003). Characterization of members of the Legionellaceae family by automated ribotyping. Journal of Clinical Microbiology 41(1), 34-43.
  7. El-Banna, N.M. (2007). Antifungal activity of Comamonas acidovorans isolated from water pond in south Jordan. African Jour- nal of Biotechnology 6(19), 2216-2219.
  8. Enright, M.C., Carter, P.E., MacLean, I.A. and McKenzie, H. (1994). Phylogenetic rela- tionships between some members of the genera Moraxella and Kingella based on partial 16S ribosomal DNA sequence analysis. International Journal of Systematic Bacteriology 44(3), 387-391.
  9. Helsel, L.O., Hollis, D.G., Steigerwalt, A.G. and Levett, P.N. (2006). Reclassification of Roseomonas fauriae Rihs et al.1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Interna- tional Journal of Systematic and Evolu- tionary Microbiology 56, 2753-2755.
  10. Hiraishi, A., Umezawa, T. Yamamoto, H., Kato, K. and Maki, Y. (1999). Changes in qui- none profiles of hot spring microbial mats with a thermal gradient. Applied and En- vironmental Microbiology 65(1), 198- 205.

Kaynak Göster

APA
Yılmaz Sarıözlü, N., Demirel, R., & Kıvanç, M. (2012). MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 2(1), 31-39. https://izlik.org/JA79AA84PD
AMA
1.Yılmaz Sarıözlü N, Demirel R, Kıvanç M. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2012;2(1):31-39. https://izlik.org/JA79AA84PD
Chicago
Yılmaz Sarıözlü, Nalan, Rasime Demirel, ve Merih Kıvanç. 2012. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2 (1): 31-39. https://izlik.org/JA79AA84PD.
EndNote
Yılmaz Sarıözlü N, Demirel R, Kıvanç M (01 Mart 2012) MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2 1 31–39.
IEEE
[1]N. Yılmaz Sarıözlü, R. Demirel, ve M. Kıvanç, “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 2, sy 1, ss. 31–39, Mar. 2012, [çevrimiçi]. Erişim adresi: https://izlik.org/JA79AA84PD
ISNAD
Yılmaz Sarıözlü, Nalan - Demirel, Rasime - Kıvanç, Merih. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2/1 (01 Mart 2012): 31-39. https://izlik.org/JA79AA84PD.
JAMA
1.Yılmaz Sarıözlü N, Demirel R, Kıvanç M. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2012;2:31–39.
MLA
Yılmaz Sarıözlü, Nalan, vd. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 2, sy 1, Mart 2012, ss. 31-39, https://izlik.org/JA79AA84PD.
Vancouver
1.Nalan Yılmaz Sarıözlü, Rasime Demirel, Merih Kıvanç. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology [Internet]. 01 Mart 2012;2(1):31-9. Erişim adresi: https://izlik.org/JA79AA84PD