AN INVESTIGATION OF MUTAGENIC ACTIVITIES OF SOME 9- SUBSTITUEDPHENANTHRENE DERIVATIVES WITH AMES / SALMONELLA / MICROSOME TEST
Öz
In this study, three different 9-Substituted phenantrene derivatives that were synthesized to be
used as basic matter for drugs were tested for their mutagenic potency in strains TA 98 and TA 100 of
Salmonella typhimurium by using Ames test. Therefore, both strains were tested in the absence or
presence of S9 metabolic activation, for five different doses of each test substances in two paralel independent
experiments. In the absence of metabolic activation, while only one of the compounds were
causing frame-shift mutations, two of them were determined to induce base pair substitution mutations.
In the presence of metabolic activation, while two of the compounds were mutagenic for TA 98,
base pair substitution mutations were not detected for any of the substances. Each of the compounds
tested were found to be mutagenic for at least one strain in the presence or absence of S9.
used as basic matter for drugs were tested for their mutagenic potency in strains TA 98 and TA 100 of
Salmonella typhimurium by using Ames test. Therefore, both strains were tested in the absence or
presence of S9 metabolic activation, for five different doses of each test substances in two paralel independent
experiments. In the absence of metabolic activation, while only one of the compounds were
causing frame-shift mutations, two of them were determined to induce base pair substitution mutations.
In the presence of metabolic activation, while two of the compounds were mutagenic for TA 98,
base pair substitution mutations were not detected for any of the substances. Each of the compounds
tested were found to be mutagenic for at least one strain in the presence or absence of S9.
Anahtar Kelimeler
Kaynakça
- Abenesi, T., Polani, S.L. ve Perticne, P. (1998). DNA strand methylation and sister chromatid exchanges in mammalian cells in vitro. Mutation Research 429, 239-248.
- Ames, B.N. ve McCann, J. (1981). Validation of the Salmonella test: A reply to Rinkus and Legator. Cancer Research 41, 4191.
- Anders, M.W. ve Dekont, W. (1994). Conjugation-dependent carcinogenicity and toxicity of foreign compounds. Advance in Pharmacology 27, 511-519.
- Arınç, E. ve Şen, A. (1994). Effect of in vivo Benzo(a)pyrene treatment on liver microsomal mixed-function oxidase activities of Gilthead seabream (Spararus aurata). Comparative Biochemistry and Physiology 107, 405-414.
- Bostrom, E., Engen, S. ve Eide, I. (1998). Mutagenicity testing of organic extracts of diesel exhaust particles after spiling with polycyclic aromotic hydrocarbons. Archives of Toxicology 72, 645-649.
- Cariello, N.F. ve Piegorsch, W.W. (1996). The Ames test: The two-fold rule revisited, Mutation Research 369, 23-31.
- Chary, M.V., Keerthysri, N.C., Vupallapati, S.V.N., Lingaiah, N. ve Kantevari, S. (2008). Tetrabutylammonium bromide (TBAB) in isopropanol: An efficient, novel, neutral and recyclable catalytic system for the synthesis of 2,4,5- trisubstituted imidazoles Catalysis Communications 9, 2013-2017.
- DeMeo, M., Vanella, P., Bernardini, E., Lagett, M., Maldonado, L., Jentzer, O., Crozet, M.P. ve Dumenill, G. (1992). Evaluation of the mutagenic and genotoxic activities of 48 nitroimidazoles and related imidazole derivatives by the Ames test and the SOS chromotest. Environmental and Molecular Mutagenesis 19(2), 167-181.
- Debnath, A.K., Compadre, R.L., Debnath, G., Shusterman, A.J. ve Hansch, G. (1991). Structure-activity relationship of mutagenic aromatic and heteroaromatic nitro compounds correlation with molecular orbital energies and hydrophobicity. Journal of Medicinal Chemistry 34, 786- 797.
- Edenharder, R., Ortseifen, M., Koch, M. ve Wesp, H.F. (2000). Soil mutagens are airborne mutagens: Variation of mutagenic activities induced in Salmonella typhimurium TA 98 and TA 100 by organic extracts of agricultural and forest soils in depence on location and season. Mutation Research 472, 23-36.