EFFECTS OF THALLIUM ON THE LARVAL DEVELOPMENT OF LUCILIA SERICATA MEIGEN 1826 AND PMI ESTIMATION

Öz

Determination of larval growth rate of and forensic analysis of the age of Calliphoridae larvae on
a corpse are useful evidence in legal investigations for the estimation of exact death time and time duration
after death; post mortem interval. However many factors, such as temperature, tissue type and
contamination of drugs and toxins, effect larval development of blow fly larvae and consequently the
estimation of post mortem interval. The present study examined the larval growth rate of a forensically
important blow fly species, Lucilia sericata Meigen 1826 in different concentrations (0,12; 0,25; 0,50;
1 and 2 μg/g) of toxic heavy metal Thallium under controlled laboratory conditions. Body length and
weight, death ratio of larvae and pupa between experimental and control groups were compared. Results
demonstrated that the development rate of larvae between uncontaminated and contaminated diets varies significantly. In short, they molted later, reached maximum length more slowly and sometimes produced significantly smaller pupae in contaminated food source. These results emphasized that the importance of determining the contamination rate of toxins in tissue for the forensic entomologist, while using development rates from standard curves based on larvae fed non-contaminated mediums.

Anahtar Kelimeler

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TALYUMUN LUCILIA SERICATA MEIGEN 1826’NIN LARVAL GELİŞIMİ VE PMI TAHMİNİ ÜZERİNE ETKİLERİ

Öz

Calliphoridae larvalarının adli analizlerle yaşlarının ve larval büyüme oranlarının belirlenmesi adli incelemelerde minimum ölüm zamanının ve ölüm sonrasında geçen sürenin ölüm sonrası zamanın belirlenmesinde kullanışlı delillerdir. Ancak sıcaklık, larvaların beslendiği doku tipi ve dokuların ilaç veya toksinlerle kontamine olması gibi birçok faktör yapışkan sinek larvalarının gelişimini dolayısıyla ölüm sonrası zamanın tahminini etkilemektedir. Bu çalışmada adli açıdan son derece önemli olan yapışkan sinek türü olan Lucilia sericata Meigen 1826’nın toksik ağır metal olan Talyumun farklı konsantrasyonlarındaki (0,12; 0,25; 0,50; 1 and 2 µg/g) gelişimi kontrol altındaki laboratuvar koşullarında incelenmiştir. Larvaların boy, ağırlık değişimi ile larval ve pupal ölüm oranları kontrol grubu ile karşılaştırılmıştır. Sonuçlar doğrultusunda, kontrol grubu ile deney gruplarının gelişimleri arasında önemli ölçüde farkların olduğu belirlenmiştir. Kısaca, kontrol grubuna oranla, daha geç deri değiştirdikleri, maksimum uzunluğa daha geç ulaştıkları ve dozaja bağlı olarak oldukça küçük pupa oluşturdukları tespit edilmiştir. Bu sonuçlar, adli çalışmalarda, standart besi ortamında yetiştirilen larval örneklerin gelişim süreleri kullanıldığında, adli araştırmacının dokulardaki kontaminasyonu belirlemesinin önemini vurgulamaktadır.

Anahtar Kelimeler

• Ölüm sonrası zamanın tahmini, Adli entomoloji, Entomotoxicology, Larval gelişim oranı, Lucilia sericata, Yapışkan sinek

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