DEVELOPMENT OF THE INTEGUMENT IN TRIFOLIUM PRATENSE L. (ELÇİ ÇAYIRÜÇGÜLÜ-FABACEAE)
Öz
This study aims to reveal the histological and cytological changes undergone by integument cells in natural tetraploid Trifolium pratense L. (Elçi Çayırüçgülü) in the stages of proembryo, zygote, mature
embryo sac and throughout ovule development.
Female organs of flowers buds and flowers sampled from those at different developmental stages were embedded both in paraffin and epon 812. The structure of integument cells was examined in the stage of mature embryo sac and throughout ovule development by a light and a transmission electron microscopy.
The ovules in natural tetraploid Trifolium pratense L. (Elçi Çayırüçgülü) have two integuments, namely inner and outer integuments. The primordia of both integuments start to appear from the medial
part, chalaza. While the megaspore mother cell is in earlier stage of Prophase I, integuments start to develop. In the stage of mature embryo sac, the inner integument is two-cells thick and it fully covers the nucellus. The outer integument becomes thicker and thicker, and one of the cells grown faster covers the junction of inner integuments and joins with the other outer integument.
It was concluded that necessary nutrients were sufficiently stored and transmitted in integument cells during the stages of ovule, embryo sac, zygote and proembryo development in natural tetraploid Trifolium pratense L. (Elçi Çayırüçgülü).
embryo sac and throughout ovule development.
Female organs of flowers buds and flowers sampled from those at different developmental stages were embedded both in paraffin and epon 812. The structure of integument cells was examined in the stage of mature embryo sac and throughout ovule development by a light and a transmission electron microscopy.
The ovules in natural tetraploid Trifolium pratense L. (Elçi Çayırüçgülü) have two integuments, namely inner and outer integuments. The primordia of both integuments start to appear from the medial
part, chalaza. While the megaspore mother cell is in earlier stage of Prophase I, integuments start to develop. In the stage of mature embryo sac, the inner integument is two-cells thick and it fully covers the nucellus. The outer integument becomes thicker and thicker, and one of the cells grown faster covers the junction of inner integuments and joins with the other outer integument.
It was concluded that necessary nutrients were sufficiently stored and transmitted in integument cells during the stages of ovule, embryo sac, zygote and proembryo development in natural tetraploid Trifolium pratense L. (Elçi Çayırüçgülü).
Anahtar Kelimeler
Kaynakça
- Algan, G. (1981). Bitkisel Dokular için Mikro- teknik. Fırat Üniversitesi Yayınları, Bot.No: 1.
- Algan, G. ve Bakar, H.N. (1990). Doğal tetraploid Çayırüçgülü (Trifolium prat- ense L.)’nde embriyo kesesi gelişmesi ve yumurta teşekkülünün incelenmesi. Turk- ish Journal of Botany 15, 57-70.
- Algan, G. ve Bakar Büyükkartal, H.N. (2000). Ultrastructure of seed coat development in the natural tetraploid Trifolium prat- ense L. Journal of Agronomy and Crop Science 184, 205-213.
- Bakar, H.N. ve Algan, G. (1997). Electron microscopic development of the endothelium in Trifolium development. Doğa Turkish Journal of Botany 21(3), 137-144. of the pratense L. during
- Borisjuk, L., Weber, H., Panitz, R., Manteuffel, R. and Wobus, U. (1995). Embryogenesis of Vicia faba L.: Histodifferentiation in relation to starch and storage protein syn- thesis. Journal of Plant Physiology 147, 203–218.
- Bouman, F. (1974). Developmental studies of the ovule, integüments and seed in some angiosperms. Thesis Univ. Amsterdam.
- Chamberlin, M.A., Horner, H.T. and Palmer, R.G. (1994). Early endosperm, embryo, and ovule development in Glycine max (L.) Merr. International Journal of Plant Science 155, 421–436.
- Colombo, L., Battaglia, R. and Kater, M.M. (2008). Arabidopsis ovule development and its evolutionary conservation. Trends Plant Science 13, 444–450.
- Elçi, Ş. (1982). The utilization of genetic re- source in fodder crop breeding, Eucarpia Fodder Crop Section 13-16 September, Aberystwyth UK.
- Galati, B.G., Rosenfeldt, S. and Tourn, M. (2006). Embryological studies in Lotus glaber (Fabaceae). Annals of Botany Fen- nici 43, 97-106.