UTILIZATION OF ANTHER CULTURE FOR SCREENING OF DOUBLED HAPLOID LINES IN SOME TURKISH BREAD WHEAT HYBRIDS

Öz

In the current research, it is aimed to investigate the doubled haploid (DH) productivity of 30 F2 derived wheat crosses constructed after hybridization of two Turkish bread wheat donors with different bread wheat genotypes, Sonmez01 and Es26. Especially, donor parents carrying out high yield performance and disease resistance selected from new developed bread wheat genotypes. Overall plant material sowed to the Transitional Zone Agricultural Research Institute field on 2010-2011 season, and an average of 40 spikes at suitable development stage harvested from each group. Under anther culture conditions, MN6 used as initial culture media and modified 190-II choosed as regeneration media. Callus obtained from two thirds of F2 hybrids and they generated 16 albino plantlets. Extended ratios of callus production ranged between 3.25% and 50.5%. However green plantlet regeneration percentage calculated as 6.25% only in one hybrid, seven hybrid wheat samples generated only 0.25% green plantlet. According to overall results, five new DH wheat formed from different F2 hybrids derived from Sonmez01, while there were any dihaploid plants observed in F2 hybrids derived from Es26. In the sum, genome and phenome based evaluation of these F2 derived five doubled haploid bread wheat hybrids can help to describe an extra population source for next generation breeding platforms that they designed to extract new resistant crops against to environmental stresses. Also, modified anther culture method used in this study might be employed for screening of other bread wheat cultivars, lines and registered genotypes in the future DH wheat population construction

Anahtar Kelimeler

• anther; albino; bread wheat; callus; doubled haploid; hybrid

BAZI TÜRK EKMEKLİK BUĞDAY MELEZLERİNDE KATLANMIŞ HAPLOİD HATLARIN TARANMASI İÇİN ANTER KÜLTÜRÜNÜN KULLANIMI

Öz

Mevcut çalışmada, iki Türk ekmeklik buğday çeşidinin (Sönmez01 ve Es26) farklı ekmeklik buğdaylarla melezlenmesi sonrası oluşturulmuş 30 F kökenli buğday çaprazının katlanmış haploid (DH) verimliliğini araştırmak amaçlanmıştır. Özellikle verici ebeveynler yüksek verimli ve hastalıklara dirençli yeni geliştirilmiş ekmeklik buğday genotiplerinden seçilmiştir. Tüm bitki materyali 2010-2011 sezonunda Geçit Kuşağı Tarımsal Araştırma Enstitüsü arazisine ekilmiş ve her gruptan gelişimin uygun evresinde ortalama 40 başak hasat edilmiştir. Anter kültürü koşulları altında, MN6 başlangıç besiyeri olarak kullanılmış, 190-II rejenerasyon besiyeri olarak seçilmiştir. F2 melezlerinin üçte ikisinden kallus elde edilmiş ve bunlar 16 albino bitkicik üretmişlerdir. Kallus üretiminin genişletilmiş oranları %3.25 ile %50.5 arasında değişmiştir. Yedi melez buğday örneği sadece %0.25 yeşil bitkicik üretmiş, ancak sadece bir melezde yeşil bitkicik rejenerasyon yüzdesi %6.25 olarak hesaplanmıştır. Es26’dan kökenlenmiş F2 melezlerinde hiç katlanmış haploid bitki gözlenmemişken, tüm sonuçlara göre, beş yeni katlanmış haploid (DH) buğday Sönmez01’den kökenlenmiş farklı F2 melezlerinden oluşmuştur. Özetle, F kökenli bu beş katlanmış haploid ekmeklik buğday melezinin genom ve fenom temelli değerlendirilmesi, çevresel streslere karşı dayanıklı yeni bitki seçmede yeni nesil ıslah platformları için dizayn edilmiş ilave bir populasyon kaynağı tanımlamaya yardımcı olabilir. Ayrıca, bu çalışmada kullanılmış modifiye anter kültürü metodu, gelecekte diğer ekmeklik buğday çeşitleri, hatları ve tescilli genotipler için katlanmış haploid (DH) ekmeklik buğday populasyonu oluşturulmasında kullanılabilir.

Anahtar Kelimeler

• Anther kültürü, Albino, Ekmeklik buğday, Kallus, Katlanmış haploid, Hibrid

Kaynakça

1. Altay, F. (2012). Yield Stability of some Turkish Winter Wheat (Triticum aestivum L.) Genotypes in The Western Transitional Zone of Turkey. Turkish Journal of Field Crops 17, 129-134.
2. Barloy, D., Denis, L. and Beckert, L. (1989). Comparison of Aptitute for Anther Culture in some Androgenetic Doubled Haploid Maize Lines. Maydica 34, 303-308.
3. Baser, I., Korkut, K.Z. and Bilgin, O. (1999). Possibilities of Obtaining Haploid Plants in Winter Bread Wheat Varities and Populations using With Anther Culture. III. November, Adana 29-34. Congress, 15-18 ISRN Agronomy
4. Can, E., Hatipoglu, R., Celiktas, N. and Genc, I. (2002). Effects of Medium and 2,4-D Concentration on Anther Culture of some Spring Wheat (Triticum aestivum L.) Genotypes. Biotechnological Equipment 16, 47-50. &
5. Chauhan, H. and Khurana, P. (2011). Use of Doubled Development of Stable Drought Tolerant Bread Wheat (Triticum aestivum L.) Transgenics. Plant Biotechnology Journal 9, 408-417, Technology for
6. Cistué, L., Romgosa, I., Batlle, F. and Echávarri, B. (2009). Improvement in The Production of Doubled Haploids in Durum Wheat (Triticum turgidum L.) Through Isolated Microspore Culture. Plant Cell Reports 28, 727-735.
7. Clapham, D. (1973). Haploid Hordeum Plants from Anthers in Vitro. Z. Pflanzenzucht. 69, 142-155.
8. De Witte, K. and Keulemans, J. (1994). Restrictions of The Efficiency of Haploid Plant Production in Apple Cultivar Idared, Through Euphytica, 77, 141-146 in Situ.

9. Dogramaci-Altuntepe, M., Peterson, T.S. and Jauhar, P.P. (2001). Anther Culture- Derived Regenerants of Durum Wheat and their Journal of Heredity 92, 56-64. Characterization.
10. Ekiz, H. and Konzak, C.F. (1997). Effects of Light Regimes on Anther Culture Response in Bread Wheat. Plant Cell Tissue and Organ Culture 50, 7-12.
11. FAO, (2004). Statistical Database, www.fao.org (Accessing date. October 1, 2013).
12. Ferrie, A.M.R. and Caswell, K.L. (2011). Isolated Microspore Culture Techniques and Recent Progress for Haploid and Doubled Haploid Plant Production. Plant Cell, Tissue and Organ Culture, 104, 301- 309
13. Grauda, D., Lepse, N., Strazdina, V., Kokina, I., Lapina, L., Mikelsone, A., Lubinskis, L. and Rashal, I. (2010). Obtaining of Doubled Haploid Lines by Anther Culture Method for The Latvian Wheat Breeding. Agronomy Research 8, 545-552.
14. Halıloglu, K. (2006). Efficient Regeneration System Segments. Biologia Plantarum 50, 326- 330. Leaf Base
15. Han, H. and Hongyuan, Y. (1986). Haploids of Higher in Vitro. Beijing, China Academic.
16. Hatipoglu, R., Genc, I. and Yagbasanlar, T. (1994). Research on Anther Culture in Bread Wheat (Triticum aestivum L.) Breeding. Field Crop Congress, 25-29 April, Izmir. 108-111.
17. Hatipoglu, R., Genc, I., Yagbasanlar, T., Ozkan, H., Celiktas, N. and Toklu, F. (1998). Production of Doubled Haploid Wheat Plants (Triticum aestivum L.) By Anther Culture. Agricoltura Mediterranea 128, 192-197.
18. Henry, Y. and De Buyser, J. (1985). Effect of The 1B/1R Translocation on Anther Culture Ability in Wheat (Triticum aestium L. ). Plant Cell Reports 4, 307-310.
19. Hofinger, B.J., Huynh, O.A., Jankowicz-Cieslak, J., Müller, A., Otto, I., Kumlehn, J. and Till, B.J. (2013). Validation of Doubled Haploid Plants By Enzymatic Mismatch Cleavage. Plant Methods 9, 43 doi:10.1186/1746-4811-9-43.
20. Hu, D., Tang, Y., Yuan, Z. and Wang, J. (1983). The Induction of Pollen Sporophyte of Winter Wheat and The Development of The New Variety Jinhua no 1. Scientia Agricultura Sinica 1, 29-35.
21. Hu, Y., Bao, R.R. and Xue, X.Y. (1988). The New Starine “764” of Spring Wheat By Pollen Haploid Technique from Anther Culture. Genetic Manipulation in Crops Newsletter 4, 70-85.
22. Islam, S.M.S. (2010). The Effect of Colchicine Pretreatment on Isolated Microspore Culture of Wheat (Triticum aestivum L.). Australian Journal of Crop Science 4, 660- 665.
23. Korkut, K.Z., Baser, I., Turhan, H. and Bilgin, O. (2001). Yerli ve Yabancı Kökenli Ekmeklik Buğday Çeşit ve Hatlarında Haploid ve Di-Haploid Genotiplerin Elde Edilme Olanakları. Trakya University, Scientifc Research Project Fund, TUAF- 232, Turkey.
24. Lantos, C., Weyen, J., Orsini, J.M., Gnad, H., Schlieter, B., Lein, V., Kontowski, S., Jacobi, A., MihÁly, R., Broughton, S. and Pauk, J. (2013). Efficient Application of in Vitro Anther Culture for Different European aestivum L.) Breeding Programmes. Plant Breeding 132, 149-154. (Triticum
25. Laurie, D.A. and Bennett, M.D. (1988). The Production of Haploid Wheat Plants from Wheat × Maize Crosses. Theoretical Applied Genetics 76, 393-397.
26. Mehmood, K., Arshad, M., Muhammad-Ali, G. and Razzaq, A. (2013). Tissue Culture Responses of some Wheat (Triticum aestivum L.) Cultivars Grown in Pakistan. Pakistan Journal of Botany 45, 545-549.
27. Mohammed, M.I. (2009). Genotype X Environment Interaction in Bread Wheat in Northern Sudan using AMMI Analysis. American-Eurasian Agricultural and Environmental Sciences 6, 427-433. Journal of
28. Murovec, J. and Bohanec B. (2012). Haploids and Doubled Haploids in Plant Breeding, Plant Abdurakhmonov (Ed.), ISBN: 978-953- 307-932-5, InTech. Dr. Ibrokhim
29. Pauk, J., Kertesz, Z., Beke, B., Bona, L., Csösz, M. and Matus, J. (1995). New Winter Wheat Variety:GK Delibab Developed Via Combining Conventional Breeding and in Vitro Androgenesis. Cereal Research Communications 23, 251-256.
30. Redden, R. (2013). New Approaches for Crop Genetic Adaptation to The Abiotic Stresses Predicted with Climate Change. Agronomy 3, 419-432.
31. Salantur, A., Yazar, S., Donmez, E. and Akar, T. Determination (2011). Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Journal of Field Crops Central Research Institute 20, 15-21. of Plant
32. Sorrells, M.E., Gustafson, J.P., Somers, D., Chao, S., Benscher, D., Guedira-Brown, G., Huttner, E., Kilian, A., McGuire, P.E., Ross, K., Tanaka, J., Wenzl, P., Williams, K. Reconstruction of The Synthetic W7984 × Opata M85 Wheat Reference Population. Genome 54, 1-8. C.O. (2011).
33. Sunderland, N. and Dunwell, J.M. (1977). Anther and Pollen Culture. In: Plant Tissue and Cell Culture (Ed. Street, H.E.) Blackwell, Oxford. TUIK, (2012). http://tuikapp.tuik.gov.tr/bitkiselapp/bitkis el.zul, (Accessing date December 15, 2013).
34. Turhan, H. and Baser, I. (2004). Callus Induction from Mature Embryo of Winter Wheat (Triticum aestivum L.). Asian Journal of Plant Sciences 3, 17-19.
35. Tuvesson, S., Ljungberg, A., Johansson, N., Karlsson, K.E., Suijs, L.W. and Josset, J.P. (2000). Large-scale Production of Wheat and Triticale Double Haploids Through The use of A Single-Anther Culture Method. Plant Breeding, 119, 455-459.
36. Yorgancilar, O., Yorgancilar, A., Bilir, O. and Yumurtaci, A. (2013). Impacts of F2 Derived Winter Bread Wheat Progenies on Callus Production and Regeneration Frequencies Through Anther Culture. Research in Plant Biology 3, 10-17.
37. Zhuang, J.J. and Jia, X. (1983). Increasing Differentiation Frequencies in Wheat Pollen Callus. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement, Science Press Peking.