Araştırma Makalesi
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DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)

Yıl 2017, Cilt: 6 Sayı: 1, 31 - 37, 16.02.2017
https://doi.org/10.18036/aubtdc.269434

Öz

The water samples collected from six stations of Stream Tersakan which include Havza; Suluova-Celtek, Kanlıdere, Bogazkoy, Karasu, Tersakan-Last and eight stations of River Yesilirmak which include Amasya Bridge, Yassıcal, Durucasu, Tasova, Kumkoy, Carsamba entrance, Carsamba Bridge, Hacıomer in Amasya and Samsun Provinces were investigated for detection of Cryptosporidium oocysts by Loop Mediated Isothermal Amplification (LAMP).

The water samples were regularly collected in every month from the 14 stations in investigated area between 2010 December to 2011 November for LAMP tests.Ten of 14 investigated stations were found contaminated with Cryptosporidium oocyst by LAMP. The pollution with Cryptosporidium oocysts were determined in stations which are Tersakan–Havza; Tersakan-Suluova, Celtek; Tersakan-Kanlıdere; Tersakan-Bogazkoy; Tersakan-Karasu Tersakan-Last, Yesilirmak-Amasya Bridge, Yesilirmak-Yassıcal, Yesilirmak-Durucasu, Yesilirmak-Tasova. The high contamination rate of Cryptosporidium oocysts were found in Tersakan–Suluova, Celtek and Tersakan-Bogazkoy stations in the all investigated stations. However, River Yesilirmak which is last in Samsun more clean than Stream Tersakan which is last in Amasya in terms of the Cryptosporidium oocysts contamination. The reason in excess of the rate of Cryptosporidium contamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water.

Kaynakça

  • [1]. Fayer R, Morgan U, Upton SJ. Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 2000; 30:1305-1322.
  • [2]. Chen X, Keithly JS, Paya CV, Russo L, Nicholos F. Kriptosporidiyoz, The New Eng. J Med 2002; 346(22): 1723-1731).
  • [3]. Smith HV, Cacciò SM, Cook N, Nichols RAB, Tait A. Cryptosporidium and Giardia as foodborne zoonoses. Vet Parasitol 2007; 149: 29–40.
  • [4]. Redlinger T, Corella-Barud V, Graham J, Galindo A, Avitia R, Cardenas V. Hyperendemic Cryptosporidium and Giardia in housholds lacking municipal sewer and water on the United States-Mexico Border. Am J Med Trop Hyg 2002; 66 (6): 794-798.
  • [5]. Karanis P, Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria. Environ Res 2006; 102: 260–71.
  • [6]. Karanis P, Kimura A. Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. Lett Appl Microbiol 2002; 34: 444–449.
  • [7]. Kourenti C, Heckeroth A, Tenter A, Karanis P. Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ microb 2003; 69: 102–106.
  • [8]. Koloren Z, Karanis P, Sotiriadou I, Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR, and LAMP in water supplies of Ordu, Middle Black Sea. Ann Trop Med Parasitol 2011; 105 (8): 607–615.
  • [9]. Plutzer J, Karanis P, Domokos K, Torokne A, Marialigeti K. Detection and characterisation of Giardia and Cryptosporidium in Hungarian raw, surface and sewage water samples by IFT, PCR and sequence analysis of the SSUrRNA and GDH genes. Int J Hyg Environ Health 2008; 211: 524–533.
  • [10]. Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, Inoue N. Development and preliminary evaluation of loop-mediated isothermal amplification (LAMP) for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl Environ microb 2007; 73: 5660–5662.
  • [11. Bakheit MA, Palomino L, Thekisoe OMM, Mate PA, Ongerth J, Karanis P. Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Vet Parasitol 2008; 158: 11–22.
  • [12]. Notomi T, Okayama H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop- mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28: 63.
  • [13]. Karanis P, Ongerth JE. LAMP - an emerging technology fort he detection of water and food borne protozoan parazites. Trends Parasitol 2009; 25:498-499.
  • [14. Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Investigations on Giardia and Cryptosporidium in drinking water supplies of Rostov region (Southern Russia) and Sofi a (Bulgaria). Environ Res. 2006; 102:475-81.
  • [15]. Alhassan A, Thekisoe OM, Yokoyama N, Inoue N, Motloang MY, Mbati PA, et al. Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis. Vet Parasitol 2007; 143: 155-60.

YEŞİLIRMAK NEHRİ ve TERSAKAN ÇAYI'NDAN (SAMSUN-AMASYA) ALINAN YÜZEYSEL SU ÖRNEKLERİNDE Cryptosporidium parvum’un LAMP TEKNİĞİYLE ARAŞTIRILMASI

Yıl 2017, Cilt: 6 Sayı: 1, 31 - 37, 16.02.2017
https://doi.org/10.18036/aubtdc.269434

Öz

Amasya il merkezi ve ilçeleri Suluova, Taşova’dan, Samsun ilçeleri Havza
ve Çarşamba’dan geçen Tersakan Çayı ve Yeşilırmak Nehri’nden alınan su
örneklerinde Cryptosporidium türleri
Loop Mediated Isothermal
Amplification (LAMP) yöntemi kullanılarak araştırılmıştır.
2010 Aralık-2011 Kasım döneminde 14 istasyondan her
ay düzenli su örnekleri alınarak bu örneklere LAMP  testi uygulanmıştır. On dört istasyonun
10’unda
Cryptosporidium ookistlerine LAMP yöntemiyle rastlanılmıştır. Cryptosporidium ookistleriyle
kontamineli istasyonlar: Tersakan–Havza; Tersakan-Suluova, Çeltek;
Tersakan-Kanlıdere; Tersakan-Boğazköy; Tersakan-Karasu; Tersakan-son;
Yeşilırmak-Amasya Köprüsü; Yeşilırmak-Yassıçal; Yeşilırmak-Durucasu;
Yeşilırmak-Taşova’dır. Araştırılan tüm istasyonların içinde Tersakan–Suluova,
Celtek ve Tersakan-Boğazköy istasyonları Cryptosporidium
ookistleri açısından en kirli istasyonlar olarak belirlenmiştir. Bununla
beraber
Amasya ve Samsun’dan geçen Yeşilırmak Nehri’nin
Tersakan Çayı’na oranla daha temiz olduğu saptanmıştır
. Tersakan Çayı’ndaki
Cryptosporidium
kontaminasyonun fazlalığı, hayvan besiciliğinin Havza and Suluova
ilçelerinde
yerleşim yerlerine çok
yakın mesafelerde yapılmasına; evsel ve tarımsal atık suların hiçbir işleme
tabi tutulmadan direk olarak Tersakan Çayı’na deşarj edilmesine bağlanmaktadır.

Kaynakça

  • [1]. Fayer R, Morgan U, Upton SJ. Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 2000; 30:1305-1322.
  • [2]. Chen X, Keithly JS, Paya CV, Russo L, Nicholos F. Kriptosporidiyoz, The New Eng. J Med 2002; 346(22): 1723-1731).
  • [3]. Smith HV, Cacciò SM, Cook N, Nichols RAB, Tait A. Cryptosporidium and Giardia as foodborne zoonoses. Vet Parasitol 2007; 149: 29–40.
  • [4]. Redlinger T, Corella-Barud V, Graham J, Galindo A, Avitia R, Cardenas V. Hyperendemic Cryptosporidium and Giardia in housholds lacking municipal sewer and water on the United States-Mexico Border. Am J Med Trop Hyg 2002; 66 (6): 794-798.
  • [5]. Karanis P, Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria. Environ Res 2006; 102: 260–71.
  • [6]. Karanis P, Kimura A. Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. Lett Appl Microbiol 2002; 34: 444–449.
  • [7]. Kourenti C, Heckeroth A, Tenter A, Karanis P. Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ microb 2003; 69: 102–106.
  • [8]. Koloren Z, Karanis P, Sotiriadou I, Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR, and LAMP in water supplies of Ordu, Middle Black Sea. Ann Trop Med Parasitol 2011; 105 (8): 607–615.
  • [9]. Plutzer J, Karanis P, Domokos K, Torokne A, Marialigeti K. Detection and characterisation of Giardia and Cryptosporidium in Hungarian raw, surface and sewage water samples by IFT, PCR and sequence analysis of the SSUrRNA and GDH genes. Int J Hyg Environ Health 2008; 211: 524–533.
  • [10]. Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, Inoue N. Development and preliminary evaluation of loop-mediated isothermal amplification (LAMP) for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl Environ microb 2007; 73: 5660–5662.
  • [11. Bakheit MA, Palomino L, Thekisoe OMM, Mate PA, Ongerth J, Karanis P. Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Vet Parasitol 2008; 158: 11–22.
  • [12]. Notomi T, Okayama H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop- mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28: 63.
  • [13]. Karanis P, Ongerth JE. LAMP - an emerging technology fort he detection of water and food borne protozoan parazites. Trends Parasitol 2009; 25:498-499.
  • [14. Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Investigations on Giardia and Cryptosporidium in drinking water supplies of Rostov region (Southern Russia) and Sofi a (Bulgaria). Environ Res. 2006; 102:475-81.
  • [15]. Alhassan A, Thekisoe OM, Yokoyama N, Inoue N, Motloang MY, Mbati PA, et al. Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis. Vet Parasitol 2007; 143: 155-60.
Toplam 15 adet kaynakça vardır.

Ayrıntılar

Bölüm Araştırma Makalesi
Yazarlar

Zeynep Kolören

Burak K. Delioğlu Bu kişi benim

Beyhan Taş

Yayımlanma Tarihi 16 Şubat 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 6 Sayı: 1

Kaynak Göster

APA Kolören, Z., Delioğlu, B. K., & Taş, B. (2017). DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA). Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 6(1), 31-37. https://doi.org/10.18036/aubtdc.269434
AMA Kolören Z, Delioğlu BK, Taş B. DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA). Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. Şubat 2017;6(1):31-37. doi:10.18036/aubtdc.269434
Chicago Kolören, Zeynep, Burak K. Delioğlu, ve Beyhan Taş. “DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 6, sy. 1 (Şubat 2017): 31-37. https://doi.org/10.18036/aubtdc.269434.
EndNote Kolören Z, Delioğlu BK, Taş B (01 Şubat 2017) DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA). Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 6 1 31–37.
IEEE Z. Kolören, B. K. Delioğlu, ve B. Taş, “DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 6, sy. 1, ss. 31–37, 2017, doi: 10.18036/aubtdc.269434.
ISNAD Kolören, Zeynep vd. “DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 6/1 (Şubat 2017), 31-37. https://doi.org/10.18036/aubtdc.269434.
JAMA Kolören Z, Delioğlu BK, Taş B. DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA). Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2017;6:31–37.
MLA Kolören, Zeynep vd. “DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 6, sy. 1, 2017, ss. 31-37, doi:10.18036/aubtdc.269434.
Vancouver Kolören Z, Delioğlu BK, Taş B. DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA). Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2017;6(1):31-7.