The Removal of Dye and Dye Stuffs Using The Bacteria Originated from Textile Industry Wastewaters

Öz

The effects of pH, initial dye concentration and incubation period on bioremoval of Remazol Blue (reactive diazo dye) was investigated in this study, with the microorganisms were isolated from textile wastewater. The strain that removed Remazol Blue most efficiently in the preliminary experiments performed at 30 °C on samples containing Remazol Blue and pH ranging from 6 to 10. The strain that removed Remazol Blue at the highest rate was selected. The optimum pH value was found as pH 8 for the strain. At constant temperature (30 °C) and initial Remazol Blue concentrations changing between 50400 mg/lt (pH 8), the strain showed its maximum dye removal rate at 50 mg/lt Remazol Blue concentration as 87.4%. The highest azoreductase activity of the selected strain was determined as 50.7 U/ml after the incubation time for 72 hours at pH 8 and 30 °C. Prolonged incubation periods decreased the enzyme activity. Ultimately the most efficient isolate has been selected

Anahtar Kelimeler

• Microorganism, Azoreductase, Remazol Blue, Wastewater

Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:

Öz

Tekstil fabrikası atıksulardan izole edilen bakterilerle yapılan bu çalışmada, pH’ın, boya konsantrasyonunun ve inkübasyon süresinin bakterilerin reaktif bir diazo boyası olan Remazol Blue giderimine etkisi araştırılmıştır. Remazol Blue içeren besiyeri ortamında pH 6–10 aralığında 30 °C de yapılan denemelerde, altı farklı izolattan en yüksek kapasitede giderimi sağlayan bir tanesi seçilmiştir. Bu bakterinin pH 8’de en fazla boya giderimi yaptığı belirlenmiştir. Bakteri sabit sıcaklıkta (30 °C), 50-400 mg/lt arasında değişen başlangıç Remazol Blue konsantrasyonlarında (pH 8) en iyi boya giderim verimini, 50 mg/lt Remazol Blue konsantrasyonunda 87.4% oranında yapmıştır. Bakterinin, 100 mg/lt Remazol Blue içeren besiyerinde giderimde etkili olduğu bilinen azoredüktaz enzim aktivitesi araştırılmış ve denemeler sonunda en yüksek enzim aktivitesi, pH 8’ de, 30 °C’de ve 72 saat inkübasyon süresi sonrasında 50.7 U/ml olarak elde edilmiştir. İnkübasyon süresinin üretilen azoredüktaz enzimi miktarına etkisine bakıldığında ise, artan inkübasyon süresinin üretilen enzim miktarında düşüşe sebep olduğu belirlenmiştir. Sonuçta giderim etkinliği en yüksek izolat seçilmiştir

Anahtar Kelimeler

• Mikroorganizma, Azoredüktaz, Remazol Blue, Atıksu

Kaynakça

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