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The Removal of Dye and Dye Stuffs Using The Bacteria Originated from Textile Industry Wastewaters

Yıl 2009, Cilt 1, Sayı 2, 75 - 82, 01.10.2009
https://doi.org/10.1501/Csaum_0000000016

Öz

The effects of pH, initial dye concentration and incubation period on bioremoval of Remazol Blue (reactive diazo dye) was investigated in this study, with the microorganisms were isolated from textile wastewater. The strain that removed Remazol Blue most efficiently in the preliminary experiments performed at 30 °C on samples containing Remazol Blue and pH ranging from 6 to 10. The strain that removed Remazol Blue at the highest rate was selected. The optimum pH value was found as pH 8 for the strain. At constant temperature (30 °C) and initial Remazol Blue concentrations changing between 50400 mg/lt (pH 8), the strain showed its maximum dye removal rate at 50 mg/lt Remazol Blue concentration as 87.4%. The highest azoreductase activity of the selected strain was determined as 50.7 U/ml after the incubation time for 72 hours at pH 8 and 30 °C. Prolonged incubation periods decreased the enzyme activity. Ultimately the most efficient isolate has been selected

Kaynakça

  • Aksu Z (2005) Application of biosorption for the removal of organic pollutants: a review. Process Biochemistry, 40: 997-1026.
  • Aksu Z and Dönmez G (2005) Combined effects of molasses sucrose and reactive dye on the growth and dye bioaccumulation properties of Candida tropicalis. Process Biochemistry, 40:1437- 1444.
  • Chang, J.S., Chou, C., Lın, Y. C., Lın, P.J., Ho, J.Y. and Hu, T.L. 2001. Kinetic characteristics of bacterial azo-dye decolorizatıon by Pseudomonas luteola. Wat. Res. 35: 2841-2850.
  • Chen, B. Y. 2002. Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics. Process Biochemistry, 38:437-446.
  • Chen, H., Wang, R.F. and Cerniglia, C.E. 2004. Molecular cloning, overexpression, purification,
  • and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expression and Purification 34:302–310.
  • Çetin, D. and Dönmez, G. 2006. Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions. Enzyme and Microbial Technology, 38: 926-930.
  • Ertuğrul, S., Bakır, M. and Dönmez, G. 2008. Treatment of dye-rich wastewater by an immobilized thermophilic cyanobacterial strain: Phormidium sp. Ecological Engineering, 32:244-248.
  • Ertuğrul, S., San, N.O. and Dönmez, G. 2009. Treatment of dye (Remazol Blue) and heavy metals using yeast cells with the purpose of managing polluted textile wastewaters. Ecological Engineering, 35:128-134.
  • Khehra, M.S., Saini, H.S., Sharma, D.K., Chadha, B.S., and Chimni, S.S. 2005. Decolorization of various azo dyes by bacterial consortium. Dyes and pigments. 67:55-61.
  • Lowry,O.H., Rosgrough,N.J., Farr,A.L., Randall,R.J. 1951. Protein measurement with the folin phenol reagent. J.Biol.Chem.193:265-275.
  • Lucas, M. S., Amaral, C., Sampaio, A., Peres, J.A. and Dias, A. A. 2006. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme and Microbial Technology, 39:51-55.
  • Martins, M.A.M., Cardoso, M.H., Queiroz, M.J., Ramalho, M.T. and. Campus, A.M.O. 1999. Biodegradation of azo dyes by the yeast Candida zeylanoides in batch aerated cultures. Chemosphere, 38:2455-2460.
  • Moutaouakkil, A., Zeroual, Y., Dzayri, F.Z., Talbi, M., Lee, K.,and Blaghena, M. 2003. Purification and partial characterization of azoreductase from Enterobacter agglomerans. Archives of Biochemistry and Biophysics, 413:139–146.
  • Nachiyar, C.V. and Rajakumar, G.S.2005. Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa. Enzyme and Microbial Technology. 36:503– 509.
  • Pandey, A., Singh, P., and Iyengar L. 2007. Bacterial decolorization and degradation of azo dyes. International Biodeterioration & Biodegradation, 59:73-84.
  • Pricelius, S., Held, C., Sollner, S., Deler, S., Murkovic, M., Ullrich, R., Hofrichter, M., Paulo, A.C., Macheroux, P., Guebitz, G.M. 2007. Enzymatic reduction and oxidation of fibre-bound azo- dyes. Enzyme and Microbial Technology 40:1732–1738.
  • Sadettin, S. and Dönmez, G. 2006. Bioaccumulation of reactive dyes by thermophilic cyanobacteria. Process Biochemistry, 41: 836-841.
  • Seesuriyachana, P., Takenakab, S., Kuntiyaa, A., Klayraungc, S., Murakamib, S. and Aokib, K. 2007. Metabolism of azo dyes by Lactobacillus casei TISTR 1500 and effects of various factors on decolorization. Water Research. 41: 985 – 992
  • Silveira, E., Marques, P.P., Silva, S.S., Lima-Filho, J.L., Porto, A.L.F., Tambourgi, E.B. 2009. Selection of Pseudomonas for industrial textile dyes decolourization. International Biodeterioration & Biodegradation, 63:230-235.
  • Wang, H., Su, J.Q., Zheng, X.W., Tian, Y., Xiong, X.J., and Zheng, T.L. 2009. Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp. CK3. International Biodeterioration & Biodegradation, in press (doi: 10.1016/j.ibiod.2008.11.006)
  • Won, S. W., Yun, H. J. and Yun, Y.S. 2009. Effect of pH on the binding mechanisms in biosorption of Reactive Orange 16 by Corynebacterium glutamicum. Journal of Colloid and Interface Science, 331:83-89.

Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:

Yıl 2009, Cilt 1, Sayı 2, 75 - 82, 01.10.2009
https://doi.org/10.1501/Csaum_0000000016

Öz

Tekstil fabrikası atıksulardan izole edilen bakterilerle yapılan bu çalışmada, pH’ın, boya konsantrasyonunun ve inkübasyon süresinin bakterilerin reaktif bir diazo boyası olan Remazol Blue giderimine etkisi araştırılmıştır. Remazol Blue içeren besiyeri ortamında pH 6–10 aralığında 30 °C de yapılan denemelerde, altı farklı izolattan en yüksek kapasitede giderimi sağlayan bir tanesi seçilmiştir. Bu bakterinin pH 8’de en fazla boya giderimi yaptığı belirlenmiştir. Bakteri sabit sıcaklıkta (30 °C), 50-400 mg/lt arasında değişen başlangıç Remazol Blue konsantrasyonlarında (pH 8) en iyi boya giderim verimini, 50 mg/lt Remazol Blue konsantrasyonunda 87.4% oranında yapmıştır. Bakterinin, 100 mg/lt Remazol Blue içeren besiyerinde giderimde etkili olduğu bilinen azoredüktaz enzim aktivitesi araştırılmış ve denemeler sonunda en yüksek enzim aktivitesi, pH 8’ de, 30 °C’de ve 72 saat inkübasyon süresi sonrasında 50.7 U/ml olarak elde edilmiştir. İnkübasyon süresinin üretilen azoredüktaz enzimi miktarına etkisine bakıldığında ise, artan inkübasyon süresinin üretilen enzim miktarında düşüşe sebep olduğu belirlenmiştir. Sonuçta giderim etkinliği en yüksek izolat seçilmiştir

Kaynakça

  • Aksu Z (2005) Application of biosorption for the removal of organic pollutants: a review. Process Biochemistry, 40: 997-1026.
  • Aksu Z and Dönmez G (2005) Combined effects of molasses sucrose and reactive dye on the growth and dye bioaccumulation properties of Candida tropicalis. Process Biochemistry, 40:1437- 1444.
  • Chang, J.S., Chou, C., Lın, Y. C., Lın, P.J., Ho, J.Y. and Hu, T.L. 2001. Kinetic characteristics of bacterial azo-dye decolorizatıon by Pseudomonas luteola. Wat. Res. 35: 2841-2850.
  • Chen, B. Y. 2002. Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics. Process Biochemistry, 38:437-446.
  • Chen, H., Wang, R.F. and Cerniglia, C.E. 2004. Molecular cloning, overexpression, purification,
  • and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expression and Purification 34:302–310.
  • Çetin, D. and Dönmez, G. 2006. Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions. Enzyme and Microbial Technology, 38: 926-930.
  • Ertuğrul, S., Bakır, M. and Dönmez, G. 2008. Treatment of dye-rich wastewater by an immobilized thermophilic cyanobacterial strain: Phormidium sp. Ecological Engineering, 32:244-248.
  • Ertuğrul, S., San, N.O. and Dönmez, G. 2009. Treatment of dye (Remazol Blue) and heavy metals using yeast cells with the purpose of managing polluted textile wastewaters. Ecological Engineering, 35:128-134.
  • Khehra, M.S., Saini, H.S., Sharma, D.K., Chadha, B.S., and Chimni, S.S. 2005. Decolorization of various azo dyes by bacterial consortium. Dyes and pigments. 67:55-61.
  • Lowry,O.H., Rosgrough,N.J., Farr,A.L., Randall,R.J. 1951. Protein measurement with the folin phenol reagent. J.Biol.Chem.193:265-275.
  • Lucas, M. S., Amaral, C., Sampaio, A., Peres, J.A. and Dias, A. A. 2006. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme and Microbial Technology, 39:51-55.
  • Martins, M.A.M., Cardoso, M.H., Queiroz, M.J., Ramalho, M.T. and. Campus, A.M.O. 1999. Biodegradation of azo dyes by the yeast Candida zeylanoides in batch aerated cultures. Chemosphere, 38:2455-2460.
  • Moutaouakkil, A., Zeroual, Y., Dzayri, F.Z., Talbi, M., Lee, K.,and Blaghena, M. 2003. Purification and partial characterization of azoreductase from Enterobacter agglomerans. Archives of Biochemistry and Biophysics, 413:139–146.
  • Nachiyar, C.V. and Rajakumar, G.S.2005. Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa. Enzyme and Microbial Technology. 36:503– 509.
  • Pandey, A., Singh, P., and Iyengar L. 2007. Bacterial decolorization and degradation of azo dyes. International Biodeterioration & Biodegradation, 59:73-84.
  • Pricelius, S., Held, C., Sollner, S., Deler, S., Murkovic, M., Ullrich, R., Hofrichter, M., Paulo, A.C., Macheroux, P., Guebitz, G.M. 2007. Enzymatic reduction and oxidation of fibre-bound azo- dyes. Enzyme and Microbial Technology 40:1732–1738.
  • Sadettin, S. and Dönmez, G. 2006. Bioaccumulation of reactive dyes by thermophilic cyanobacteria. Process Biochemistry, 41: 836-841.
  • Seesuriyachana, P., Takenakab, S., Kuntiyaa, A., Klayraungc, S., Murakamib, S. and Aokib, K. 2007. Metabolism of azo dyes by Lactobacillus casei TISTR 1500 and effects of various factors on decolorization. Water Research. 41: 985 – 992
  • Silveira, E., Marques, P.P., Silva, S.S., Lima-Filho, J.L., Porto, A.L.F., Tambourgi, E.B. 2009. Selection of Pseudomonas for industrial textile dyes decolourization. International Biodeterioration & Biodegradation, 63:230-235.
  • Wang, H., Su, J.Q., Zheng, X.W., Tian, Y., Xiong, X.J., and Zheng, T.L. 2009. Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp. CK3. International Biodeterioration & Biodegradation, in press (doi: 10.1016/j.ibiod.2008.11.006)
  • Won, S. W., Yun, H. J. and Yun, Y.S. 2009. Effect of pH on the binding mechanisms in biosorption of Reactive Orange 16 by Corynebacterium glutamicum. Journal of Colloid and Interface Science, 331:83-89.

Ayrıntılar

Diğer ID JA34TY45SS
Bölüm Makale
Yazarlar

Sevgi ERTUĞRUL Bu kişi benim


Gönül DÖNMEZ Bu kişi benim

Yayımlanma Tarihi 1 Ekim 2009
Yayınlandığı Sayı Yıl 2009, Cilt 1, Sayı 2

Kaynak Göster

Bibtex @ { aucevrebilim477388, journal = {Ankara Üniversitesi Çevrebilimleri Dergisi}, eissn = {2146-1562}, address = {csaum@ankara.edu.tr}, publisher = {Ankara Üniversitesi}, year = {2009}, volume = {1}, number = {2}, pages = {75 - 82}, doi = {10.1501/Csaum\_0000000016}, title = {Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:}, key = {cite}, author = {Ertuğrul, Sevgi and Dönmez, Gönül} }
APA Ertuğrul, S. & Dönmez, G. (2009). Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı: . Ankara Üniversitesi Çevrebilimleri Dergisi , 1 (2) , 75-82 . DOI: 10.1501/Csaum_0000000016
MLA Ertuğrul, S. , Dönmez, G. "Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:" . Ankara Üniversitesi Çevrebilimleri Dergisi 1 (2009 ): 75-82 <https://dergipark.org.tr/tr/pub/aucevrebilim/issue/40126/477388>
Chicago Ertuğrul, S. , Dönmez, G. "Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:". Ankara Üniversitesi Çevrebilimleri Dergisi 1 (2009 ): 75-82
RIS TY - JOUR T1 - Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı: AU - SevgiErtuğrul, GönülDönmez Y1 - 2009 PY - 2009 N1 - doi: 10.1501/Csaum_0000000016 DO - 10.1501/Csaum_0000000016 T2 - Ankara Üniversitesi Çevrebilimleri Dergisi JF - Journal JO - JOR SP - 75 EP - 82 VL - 1 IS - 2 SN - -2146-1562 M3 - doi: 10.1501/Csaum_0000000016 UR - https://doi.org/10.1501/Csaum_0000000016 Y2 - 2022 ER -
EndNote %0 Ankara Üniversitesi Çevrebilimleri Dergisi Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı: %A Sevgi Ertuğrul , Gönül Dönmez %T Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı: %D 2009 %J Ankara Üniversitesi Çevrebilimleri Dergisi %P -2146-1562 %V 1 %N 2 %R doi: 10.1501/Csaum_0000000016 %U 10.1501/Csaum_0000000016
ISNAD Ertuğrul, Sevgi , Dönmez, Gönül . "Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:". Ankara Üniversitesi Çevrebilimleri Dergisi 1 / 2 (Ekim 2009): 75-82 . https://doi.org/10.1501/Csaum_0000000016
AMA Ertuğrul S. , Dönmez G. Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:. Ankara Üniversitesi Çevrebilimleri Dergisi. 2009; 1(2): 75-82.
Vancouver Ertuğrul S. , Dönmez G. Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:. Ankara Üniversitesi Çevrebilimleri Dergisi. 2009; 1(2): 75-82.
IEEE S. Ertuğrul ve G. Dönmez , "Tekstil Atıksuyu Kaynaklı Bakterilerin Boya ve Boyar Madde Arıtımında Kullanımı:", Ankara Üniversitesi Çevrebilimleri Dergisi, c. 1, sayı. 2, ss. 75-82, Eki. 2009, doi:10.1501/Csaum_0000000016